Journal of Medicinal Plants

en The Effects of Taraxacum officinale L. and Berberis vulgaris L. Root Extracts on Carbon Tetrachloride Induced Liver Toxicity in Rats The Effects of Taraxacum officinale L. and Berberis vulgaris L. Root Extracts on Carbon Tetrachloride Induced Liver Toxicity in Rats فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research Background: Taraxacum officinale and Berberis vulgaris have long been used as herbal remedies for treatment of a variety of complaints including liver dysfunction and gallbladder disease. However scientifically reliable data are needed to verify their minimum effective doses. Objective: In present study, the effects of Taraxacum officinale L. and Berberis vulgaris L. root extracts at the different doses 10, 20 and 30 times higher than average dose (THD) used in traditional systems of medicines were tested against carbon tetrachloride (CCl4) induced liver toxicity in rats. Methods: The root extracts of T. officinale at doses of 250, 500 and 750 mg/kg/day and B. vulgaris at doses of 300, 600 and 900 mg/kg/day, relative to 10, 20 and 30 THD average doses used in traditional systems of medicines were prepared by dissolving dry extracts in 5% dimethyl sulfoxide in distilled water. Eighty male Wistar rats, 5 months old, were divided in 8 groups of 10 rats each. Liver intoxication was induced in 7 groups by intraperitoneal injection of 1 ml/kg of 1:1 CCl4 in olive oil for two successive days. One group was kept as control and six different doses of medicinal plants extracts were administered to six groups simultaneously with CCl4 administration. After three days the serum levels of ALT, AST and ALP, liver tissue glutathione level and catalase activities as well as liver tissue microvesicular steatosis and pericentral coagulation necrosis were determined. Results: In control group the blood levels of ALT, AST, ALP and liver tissue injury were increased whereas the serum GSH level and catalase activity decreased significantly after 3 days of beginning of carbon tetrachloride liver toxicity as compared to normal group. In T. officinale treated group at the dose of 750 mg/kg/day, the serum ALT and ALP levels and in B. vulgaris at the dose of 900 mg/kg/day, the serum ALP levels reduced significantly as compared to control group. The liver micro vesicular steatosis was inhibited significantly in both groups at the doses of 30 THD as compared to control group. <div dir="ltr" style="text-align: justify;">Conclusion: In the present study administration of T. officinale and B. vulgaris root extracts at with 30 THD ameliorated CCl4 induced liver damage.</div> Background: Taraxacum officinale and Berberis vulgaris have long been used as herbal remedies for treatment of a variety of complaints including liver dysfunction and gallbladder disease. However scientifically reliable data are needed to verify their minimum effective doses. Objective: In present study, the effects of Taraxacum officinale L. and Berberis vulgaris L. root extracts at the different doses 10, 20 and 30 times higher than average dose (THD) used in traditional systems of medicines were tested against carbon tetrachloride (CCl4) induced liver toxicity in rats. Methods: The root extracts of T. officinale at doses of 250, 500 and 750 mg/kg/day and B. vulgaris at doses of 300, 600 and 900 mg/kg/day, relative to 10, 20 and 30 THD average doses used in traditional systems of medicines were prepared by dissolving dry extracts in 5% dimethyl sulfoxide in distilled water. Eighty male Wistar rats, 5 months old, were divided in 8 groups of 10 rats each. Liver intoxication was induced in 7 groups by intraperitoneal injection of 1 ml/kg of 1:1 CCl4 in olive oil for two successive days. One group was kept as control and six different doses of medicinal plants extracts were administered to six groups simultaneously with CCl4 administration. After three days the serum levels of ALT, AST and ALP, liver tissue glutathione level and catalase activities as well as liver tissue microvesicular steatosis and pericentral coagulation necrosis were determined. Results: In control group the blood levels of ALT, AST, ALP and liver tissue injury were increased whereas the serum GSH level and catalase activity decreased significantly after 3 days of beginning of carbon tetrachloride liver toxicity as compared to normal group. In T. officinale treated group at the dose of 750 mg/kg/day, the serum ALT and ALP levels and in B. vulgaris at the dose of 900 mg/kg/day, the serum ALP levels reduced significantly as compared to control group. The liver micro vesicular steatosis was inhibited significantly in both groups at the doses of 30 THD as compared to control group. <div style="text-align: justify;">Conclusion: In the present study administration of T. officinale and B. vulgaris root extracts at with 30 THD ameliorated CCl4 induced liver damage.</div> <,i>,Taraxacum officinale<,/i>,, <,i>,Berberis vulgaris<,/i>,, Medicinal plants, Liver toxicity, Carbon tetrachloride <,i>,Taraxacum officinale<,/i>,, <,i>,Berberis vulgaris<,/i>,, Medicinal plants, Liver toxicity, Carbon tetrachloride 45 52 http://jmp.ir/browse.php?a_code=A-10-90-6&slc_lang=en&sid=1 H Fallah Huseini حسن فلاح حسینی huseini_fallah@yahoo.com 100319475328460038102 100319475328460038102 Yes Department of Pharmacology and Applied Medicine, Institute of Medicinal Plants ACECR, Karaj Department of Pharmacology and Applied Medicine, Institute of Medicinal Plants ACECR, Karaj A Zareei Mahmoudabady زارعی محمدآبادی 100319475328460038103 100319475328460038103 No Department of Biochemistry, Baqyiatallah University of Medical Sciences, Tehran Department of Biochemistry, Baqyiatallah University of Medical Sciences, Tehran SA Ziai سیدعلی ضیایی 100319475328460038104 100319475328460038104 No Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University, Tehran Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University, Tehran M Mehrazma مهرآزما 100319475328460038105 100319475328460038105 No Department of Pathology Iran University of Medical Sciences, Tehran Department of Pathology Iran University of Medical Sciences, Tehran SM Alavian سیدموید علویان 100319475328460038106 100319475328460038106 No Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran S Kianbakht سعید کیان بخت 100319475328460038107 100319475328460038107 No Department of Pharmacology and Applied Medicine, Institute of Medicinal Plants ACECR, Karaj Department of Pharmacology and Applied Medicine, Institute of Medicinal Plants ACECR, Karaj M Mehdizadeh مهدی زاده 100319475328460038108 100319475328460038108 No Department of Anatomical Sciences, Cellular and Molecular Research Center, Iran University of Medical Sciences Tehran Department of Anatomical Sciences, Cellular and Molecular Research Center, Iran University of Medical Sciences Tehran