en Identification and Quantitative Determination of 4-Hydroxyisoleucine in <i>Trigonella foenum-graecum</i> L. from Iran فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <p dir="ltr" style="text-align: justify;">&nbsp;<strong> Background:</strong> The seeds of <i>Trigonella foenum-graecum </i>L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine.</p> <p dir="ltr" style="text-align: justify;">&nbsp; <strong>Objective:</strong> In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated.</p> <p dir="ltr" style="text-align: justify;">&nbsp; <strong>Methods:</strong> Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique.</p> <p dir="ltr" style="text-align: justify;">&nbsp;<strong> Results:</strong> The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> According to the effects of 4-hydroxyisoleucine such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders.</div> <p style="text-align: justify;">&nbsp;<strong> Background:</strong> The seeds of <i>Trigonella foenum-graecum </i>L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine.</p> <p style="text-align: justify;">&nbsp; <strong>Objective:</strong> In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated.</p> <p style="text-align: justify;">&nbsp; <strong>Methods:</strong> Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique.</p> <p style="text-align: justify;">&nbsp;<strong> Results:</strong> The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran.</p> <div style="text-align: justify;"><strong>Conclusion:</strong> According to the effects of 4-hydroxyisoleucine such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders.</div> <,i>,Trigonella foenum-graecum<,/i>,, Amino acid, Diabetes, 4-hydroxyisoleucine <,i>,Trigonella foenum-graecum<,/i>,, Amino acid, Diabetes, 4-hydroxyisoleucine 29 34 http://jmp.ir/browse.php?a_code=A-10-319-1&slc_lang=en&sid=1 H Hajimehdipoor هما حاجی مهدی پور 100319475328460038064 100319475328460038064 No Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran SE Sadat-Ebrahimi سیدابراهیم سادات ابراهیمی sesebrahimi@sina.tums.ac.ir 100319475328460038065 100319475328460038065 Yes Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran Y Amanzadeh امن زاده 100319475328460038066 100319475328460038066 No Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran M Izaddoost محمد ایزددوست 100319475328460038067 100319475328460038067 No Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran E Givi گیوی 100319475328460038068 100319475328460038068 No Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran