Journal of Medicinal Plants

en Extraction and nano-encapsulation of coumarin for drug-delivery in a biodegradable scaffold with impact on L929 cell proliferation فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div style="text-align: justify;">Background: Nano-encapsulation can control drug release and promote cell proliferation, offering significant benefits for tissue engineering in medical applications. Objective: This study focused on developing coordinated nanofibers made of gelatin/PVA and PCL nanocapsules loaded with coumarin. We aimed to evaluate (a) the platform's ability to control drug delivery, (b) its biocompatibility, and (c) its effect on the expansion of L929 cells during the exponential and adaptation growth phases. Methods: Coumarin was extracted from the Melilotus officinalis L. and nano-encapsulated using polycaprolactone (PCL). The nano-encapsulated coumarin was coated by electrospinning with polyvinyl alcohol (PVA) and gelatin to form nanofibers, chosen for their ECM-like properties. Various analytical techniques, including FTIR, 1H NMR, SEM, UV, mechanical testing, HRTEM, DSC, and HPLC, were used to evaluate the results. Cell proliferation and biological effects were assessed using the MTT method at days 1, 3, and 5. Results: PVA and gelatin provided hydrophilic properties that support optimal cell adhesion, proliferation, and function in the electrospun nanofibers. Drug release behavior showed a slower release rate in neutral environments compared with alkaline or acidic conditions, indicating pH-dependent release characteristics. No cytotoxicity was observed during evaluation, suggesting good biocompatibility of the scaffold system. Conclusion: The combination of gelatin/PVA electrospun nanofibers and PCL-loaded coumarin nanocapsules demonstrates potential as a synergistic nano-delivery system. The observed delayed drug release aligned with growth phases of the L929 cells, supporting applications in tissue engineering where controlled release and biocompatibility are essential.</div> Nano-encapsulation, Drug release, Electrospun Nanofibers, Coumarin, L929 cells 60 76 http://jmp.ir/browse.php?a_code=A-10-7042-2&slc_lang=en&sid=1 Rojan Akhbarati rojan.akhbarati@gmail.com 100319475328460049196 0009-0002-0413-6047 No Department of Chemistry, C. T. C, Islamic Azad University, Tehran, Iran Rahebeh Amiri Dehkharghani Rah.Amiri@iauctb.ac.ir 100319475328460049197 100319475328460049197 Yes Department of Chemistry, C. T. C, Islamic Azad University, Tehran, Iran Majid Ghorbani Nohooji m.gh.nahooji@gmail.com 100319475328460049198 100319475328460049198 No Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran