en فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div style="text-align: justify;"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background: </span></span></b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Morinda citrifolia</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> L. possesses antioxidant activity that can ameliorate the decline in semen quality of male rats due to exposure to cigarette smoke. <b>Objectives</b>: This study intend to assess the effectiveness of <i>M._citrifolia</i> leaves extract in ameliorating male infertility associated with oxidative dysregulation induced by exposure to tobacco smoke. <b>Methods</b>: The animals used in the study were evenly and randomly divided into five groups, each containing five rats. Group X1 served as the normal control without any treatment, whereas group X2 comprised rats that were exclusively subjected to cigarette smoke exposure. Groups X3, X4, and X5 were exposed to cigarette smoke and subsequently administered <i>M. citrifolia </i>leaves extract orally via a nasogastric tube at doses of 100, 200, and 300 mg/kg BW, respectively, for a period of 52 days. Twenty-four hours after the final treatment, blood samples were collected to examine FSH, LH, and testosterone levels using ELISA technique. Semen was collected from the cauda epididymis to analyze the quality of spermatozoa. <b>Results</b>: The administration of <i>M. citrifolia </i>leaves extract improved sperm concentration, progressive motility, and viability, while sperm morphological abnormalities were not affected by the extract (P = 0.618). FSH concentration decreased following <i>M. citrifolia </i>leaves extract administration, particularly at dose of 100, and 200 mg/kg BW. LH concentration increased significantly after treatment with 100_mg/kg BWof <i>M. citrifolia </i>leaves extract and testosterone levels improved after treated with leaves extract of <i>M. citrifolia </i>(P <0.001). <b>Conclusions</b>: Methanol extract of <i>M. citrifolia </i>leaves enhance sperm quality and testosterone levels but does not affect FSH and LH concentrations in male rats exposed to cigarette smoke.</span></span></div> Cigarette smoke, FSH, LH, M. citrifolia L., Spermatozoa, Testosterone 68 78 http://jmp.ir/browse.php?a_code=A-10-7031-1&slc_lang=en&sid=1 Ratna Dewi dewiratnapoltekkesaceh@gmail.com 100319475328460049005 0009-0005-9472-2091 No Department of Mathematics and Sciences Application, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia; Department of Midwifery, Aceh Health Polytechnic, Ministry of Health, Aceh Besar, Indonesia Tongku Nizwan Siregar siregar@usk.ac.id 100319475328460049006 100319475328460049006 Yes Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia Amalia Sutriana amalia_sutriana@usk.ac.id 100319475328460049007 100319475328460049007 No Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia Sri Wahyuni sri_wahyuni@usk.ac.id 100319475328460049008 0009-0004-3230-7012 No Laboratory of Anatomy, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia Teuku Armansyah t_armansyah@usk.ac.id 100319475328460049009 100319475328460049009 No Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia