en فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div style="text-align: justify;"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b> <i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Citrus sinensis</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">, popularly called sweet orange, is widely used in Brazil for its calming properties, particularly in treating anxiety and insomnia, even among patients using warfarin. However, there is no scientific data supporting the safe concurrent use of both. <b>Objective:</b> To access the in vitro activity of </span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">C. sinensis</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> ethanolic extract (EtEXT) and its fractions on blood clotting. <b>Methods:</b> The study used activated partial thromboplastin time (aPTT), prothrombin time (PT) and plasma fibrinogen measurement (PF), and thrombin generation test (TGT) to evaluate the effects. TGT, a highly sensitive assay, investigates overall changes in the hemostatic system and can evaluate the qualitative micromolecular chemistry of </span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">C. sinensis</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> EtEXT and its fractions. <b>Results:</b> The EtEXTs of sweet orange leaves and their fractions were added to plasma pools at concentrations of 1.67 mg/ml, 2.26 mg/ml, and 2.86 mg/ml. Presence of phenolics as coumarins, flavonoids and tannins, as well as triterpenes was confirmed. At all concentrations, the extract increased PT and aPTT while decreasing PF and TGT, except for the hexane fraction. <b>Conclusion:</b> Our findings provide scientific evidence supporting both patient care in the context of warfarin use and the potential development of new antithrombotics. The leaves of sweet orange exhibited in vitro anticoagulant effects, likely due to interference in both extrinsic (EXT-path) intrinsic (INT-path) coagulation pathways. The function of the identified substances in these effects was also discussed, as well as the potential for using this extract in developing antithrombotic agents.</span></span></div> Anticoagulants, Herbal medicine, Citrus, Thrombin generation, Warfarin 53 67 http://jmp.ir/browse.php?a_code=A-10-7200-1&slc_lang=en&sid=1 Paula Mendonça Leite paulamleite02@gmail.com 100319475328460048998 100319475328460048998 No Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil Ana Paula Nader Miranda ananader86@gmail.com 100319475328460048999 100319475328460048999 No Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil Juliana Mendes Amorim juliana.amorimf@gmail.com 100319475328460049000 100319475328460049000 No Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil Rita Carolina Figueiredo Duarte ritinhaduarte@yahoo.com.br 100319475328460049001 100319475328460049001 No Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil André Augusto Gomes Faraco andrefaraco@farmacia.ufmg.br 100319475328460049002 100319475328460049002 No Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil Maria das Graças Carvalho mgcarvalho@farmacia.ufmg.br 100319475328460049003 100319475328460049003 No Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil Rachel Oliveira Castilho roc2006@farmacia.ufmg.br 100319475328460049004 100319475328460049004 Yes Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil