en فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div style="text-align: justify;"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Background:</span></span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt"> Historical documents highlight various multifocal products for wound management, including the <i>Zaroor Arbaee</i> formulation, which comprises <i>Commiphora myrrha</i>, <i>Boswellia</i> sp., <i>Astragalus fasciculifolius</i>, and <i>Dracaena</i> sp. <b>Objective:</b> This study investigates a novel topical formulation for skin wound healing based on Traditional Persian Medicine, emphasizing these botanicals. <b>Methods:</b> A methanol extract was prepared, and an MTT assay was used to assess cytotoxicity on normal liver cells. Antimicrobial activity was evaluated using the microdilution method according to CLSI guidelines. A 5% topical cream was formulated, and total phenolic, flavonoid, and tannin contents were measured via spectrophotometry. High-performance thin-layer chromatography (HPTLC) was employed for phytochemical profiling, and essential oil extraction was performed to identify volatile constituents. Organoleptic and physicochemical tests were also conducted on the cream. <b>Results:</b> The MTT assay showed no cytotoxicity, and antimicrobial tests confirmed significant antifungal activity. Total phenolic, flavonoid, and tannin contents were 7.59 &plusmn; 0.31 mg, 0.54 &plusmn; 0.007 mg, and 2.07 &plusmn; 0.13 mg per 1.5 g of extract in a 30 g cream, respectively. Essential oil analysis identified <i>furanoeudesma-1,3-diene</i> (32.84%) and <i>curzerene</i> (14.94%) as major constituents. Additional assessments confirmed the cream&rsquo;s stability and spreadability. <b>Conclusion:</b> <i>Zaroor Arbaee</i> appears to be a promising traditional remedy for skin wound healing.</span></span></span></div> Wound healing, Commiphora myrrha, Boswellia, Astragalus fasciculifolius, Dracaena 1 18 http://jmp.ir/browse.php?a_code=A-10-2501-5&slc_lang=en&sid=1 Maryam Falahatzadeh maryamfalahatzademf@gmail.com 100319475328460049018 0009-0005-8139-8392 No Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Zahra Sobhani sobhani@sums.ac.ir 100319475328460049019 100319475328460049019 No Department of Quality Control, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Kamiar Zomorodian zomorodian@sums.ac.ir 100319475328460049020 100319475328460049020 No Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Seyedeh Sara Hashemi sara.hashemi1389@gmail.com 100319475328460049021 100319475328460049021 No Department of Histology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Mohammad M. Zarshenas zarm@sums.ac.ir 100319475328460049022 100319475328460049022 Yes Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran