en بیان ژن‌های آپوپتوز ناشی از عصاره بذر کتان در رده سلولی سرطان پروستات انسان گياهان دارویی Medicinal Plants پژوهشی Research <div style="text-align: justify;"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">: Prostate cancer is known as the second common cancer among men, and is introduced as the fifth cause of death. Apoptosis known as &ldquo;Programmed Cell Death&rdquo;, can be measured as a defense mechanism in response to cell damage, caused by diseases or exposure to toxic material, and has an important role in controlling cell population. For this, researchers are interested in studying cancer treatment for inducing apoptosis. Flaxseed, <i>Linum usitassimum</i>, has numerous benefits among other oilseeds. It contains a large amount of &alpha;-Linoleic Acid (ALA), dietary fiber, proteins, and Phytoestrogen. <b>Objective</b>: The main goal of this research was to evaluate the expression of apoptotic genes induced with flaxseed extract in human prostate cancer cells in order to find out the cell death pathway induced by flaxseed extract. <b>Methods:</b> In this study, PC-3 prostate cancer cells were cultured and the treatments were done using the flaxseed extract. The MTT test, IC50 calculations, Flowcytometry, and Real-Time PCR test for apoptotic genes were done and all the results underwent analysis. <b>Results</b>: The IC50 dose was obtained at 809 &micro;g/ml (P < 0.05). Flowcytometry test was done for 600, 800, and 1000 &micro;g/ml of extraction, and the result showed that most of the cells underwent necrosis. The Flowcytometry result was confirmed by qPCR test and showed the overexpression of TNF gene (P < 0.05). </span></span><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">: The results showed the overexpression of all the studied genes after treatment by flaxseed extract. The Flowcytometry result was confirmed by qPCR and showed that the TNF gene was overexpressed.</span></span></div> Prostate cancer, PC-3 cell line, Flaxseed, Apoptosis, Gene Expression 57 67 http://jmp.ir/browse.php?a_code=A-10-6883-1&slc_lang=en&sid=1 Maryam Hakimi مریم حکیمی sara-hakimi@live.com 100319475328460048407 100319475328460048407 No Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran گروه بیولوژی سلولی و مولکولی، دانشکده علوم زیستی و بیوتکنولوژی، دانشگاه شهید بهشتی، تهران، ایران Reza Shiari رضا شیاری shiareza@yahoo.com 100319475328460048408 100319475328460048408 No Department of Pediatric Rheumatology, Shahid Beheshti University of Medical Sciences, Tehran, Iran گروه اطفال، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران Afshan Shirkavand افشان شیرکوند a_shirkavand@sbu.ac.ir 100319475328460048409 100319475328460048409 No Department of Photodynamic, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran گروه فتودینامیک، مرکز تحقیقات لیزر پزشکی، پژوهشکده یارا، سازمان جهاد دانشگاهی علوم پزشکی تهران، تهران، ایران Mohammadreza Razaghi محمدرضا رزاقی m-razaghi@sbmu.ac.ir 100319475328460048410 100319475328460048410 No Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات لیزر در علوم پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران Shirin Farivar شیرین فریور s_farivar@sbu.ac.ir 100319475328460048411 100319475328460048411 Yes Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran گروه زیست‌شناسی سلولی و مولکولی، دانشکده علوم زیستی و بیوتکنولوژی، دانشگاه شهید بهشتی، تهران، ایران