en گياهان دارویی Medicinal Plants پژوهشی Research <div style="text-align: justify;"><b><span lang="EN-ZW" style="border:none windowtext 1.0pt; font-size:10.0pt; padding:0cm"><span style="background:#fbfbfb"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background:</span></span></span></span></b> <i><span style="border:none windowtext 1.0pt; font-size:10.0pt; padding:0cm"><span style="background:#fbfbfb"><span new="" roman="" style="font-family:" times=""><span style="color:black">Malva neglecta</span></span></span></span></i><span style="border:none windowtext 1.0pt; font-size:10.0pt; padding:0cm"><span style="background:#fbfbfb"><span new="" roman="" style="font-family:" times=""><span style="color:black"> is a commonly used medicinal plant known for its content of tannins, hydroxycinnamic acids, alkaloids, flavonoids, saponins, anthocyanins, and flavonols. <b>Objective: </b>The objective of this research was to assess the effects of <i>M. neglecta</i> extract on male rat infertility. <b>Methods: </b>Forty adult male rats were divided into eight groups. The <i>M. neglecta</i> extract was administered daily for 28 days. At the conclusion of the treatment period, all animals were anesthetized with ether. Following euthanasia, dissection was performed, and sperm morphology, viability, and motility were assessed in the epididymis. The right testis was subjected to histological analysis after tissue processing and H/E staining, with cell counts conducted under a light microscope. The left testis was examined for superoxide dismutase (SOD) and malondialdehyde (MDA) enzyme levels. <b>Results: </b>Sperm analysis showed a significant improvement in viability, motility, and concentration in the infertile groups treated with <i>M. neglecta</i> extract at doses of 100, 200, and 400 mg/kg. SOD enzyme levels were notably higher in the experimental groups receiving the extract, surpassing those of the healthy control group. Furthermore, increasing the extract dosage led to a rise in SOD production within the infertile groups. MDA levels, an indicator of lipid peroxidation, were lower in the experimental groups compared to the control group, with the MDA concentration nearly identical to that of the healthy controls. This suggests that <i>M. neglecta</i> extract significantly reduced lipid peroxidation. <b>Conclusion</b>: The findings indicate that <i>M. neglecta</i> extract has a notable positive effect on spermatogenesis, sperm viability, motility, and concentration, particularly under oxidative stress conditions.</span></span></span></span></div> Oxidative stress, Induced infertility, Animal modelling, Sperm factors, Histopathology 46 56 http://jmp.ir/browse.php?a_code=A-10-6837-1&slc_lang=en&sid=1 Fatemeh Sadrforati sadr.f.fatemeh@gmail.com 100319475328460048564 100319475328460048564 Yes Department of Veterinary Medicine, Faculty of Veterinary Medicine, Science and Research Branch of Islamic Azad University, Tehran, Iran Pejman Mortazavi sp.mortazavi@srbiau.ac.ir 100319475328460048565 100319475328460048565 No Department of Pathology, Faculty of Veterinary Medicine, Science and Research Branch of Islamic Azad University, Tehran, Iran Mohammad Amin Eslampour maeslampour@srbiau.ac.ir 100319475328460048566 100319475328460048566 No Department of Theriogeology, Faculty of Veterinary Medicine, Science and Research Branch of Islamic Azad University, Tehran, Iran