en بررسی اثر ضد باکتریایی عصاره و اسانس صمغ اولئو-رزین گیاه <i>Ferula foetida</i> (Bunge) Regel (گندکما) بر روی <i>Staphylococcus aureus</i> مقاوم به متی سیلین و اثر همزمان آن با وانکومایسین فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div style="text-align: justify;"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background: </span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Nowadays, the decreased susceptibility of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) to last-resort antibiotics such as vancomycin has caused concern in both human and veterinary medicine. Therefore, the need to find alternative treatments and new antibacterial agents is felt more than ever. <b>Objective: </b>The antibacterial effect and simultaneous effect of <i>Ferula foetida</i> (Bunge) Regel (<i>F. foetida</i>) oleo-gum-resin (OGR) extract and vancomycin on methicillin-resistant <i>Staphylococcus aureus </i>(MRSA) were investigated.<b> Methods: </b>Aqueous, ethanolic, a mixture of aqueous and ethanolic extracts, and essential oil were prepared from <i>F. foetida</i> OGR collected from Beyrut village, <span style="background:white">Sabzevar, Razavi Khorasan province, Iran</span>. The Checkerboard method was used to determine the minimum inhibitory concentrations (MICs) of vancomycin and plant extracts and essential oil, and their simultaneous effects on MRSA (ATCC 33591). The minimum bactericidal concentrations (MBCs) of these agents were also determined. Ethanolic extract and essential oil of <i>F. foetida</i> OGR were analyzed using <span style="background:white"><span style="color:#202122">gas chromatography-mass spectrometry</span></span> (GC-MS). <b>Results: </b>The MICs of vancomycin and aqueous, ethanolic, a mixture of aqueous and ethanolic extracts, and essential oil of <i>F. foetida</i> OGR were 0.00195 mg/ml, 8 mg/ml, 0.25 mg/ml, 0.5 mg/ml, and 256 mg/ml, respectively. The MBCs of these agents were 0.0078 mg/ml, 64 mg/ml, 0.5 mg/ml, 2 mg/ml, and more than 512 mg/ml, respectively. The simultaneous use of the extracts and essential oil of this plant with vancomycin on MRSA showed partial synergistic to additive effects. </span></span><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">:</span></span></b> <span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The combinations of </span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">F. foetida</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> OGR </span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">extracts or essential oil with vancomycin are expected to reduce the effective dose of vancomycin against MRSA.</span></span></div> Methicillin-resistant Staphylococcus aureus, Ferula foetida, Vancomycin, Antibacterial effect, Checkerboard 82 93 http://jmp.ir/browse.php?a_code=A-10-6836-1&slc_lang=en&sid=1 Zahra Gholizadeh Farshi زهرا قلی‎زاده فرشی za00gh@gmail.com 100319475328460047767 100319475328460047767 No Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran بخش پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران Zahra Naziri زهرا نظیری z.naziri@shirazu.ac.ir 100319475328460047768 100319475328460047768 Yes Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran بخش پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران Abdollah Derakhshandeh عبدالله درخشنده drkhshnd77@gmail.com 100319475328460047769 100319475328460047769 No Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran بخش پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران Fatemeh Raouf Fard فاطمه رؤف‎فرد fraouffard@yahoo.com 100319475328460047770 100319475328460047770 No Department of Horticultural Sciences, School of Agriculture, Shiraz University, Shiraz, Iran بخش علوم باغبانی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران