en بهینه‎ سازی استخراج پلی‎ فنل از ریشه‌ های <i>Anacyclus pyrethrum</i> var. <i>depressus</i>: یک رویکرد طراحی مخلوط سیپلکس مرکزی گياهان دارویی Medicinal Plants پژوهشی Research <div style="text-align: justify;"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background</span></span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">:<i> </i></span></span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Anacyclus</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> <i>pyrethrum</i> var. <i>depressus</i> (Ball) Maire</span></span> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">is a medicinal plant whose roots have been reported to possess medicinal properties in traditional and modern medicine systems. <b>Objective</b>: The present work aims to maximize the extraction of polyphenols and compounds with antioxidant properties by applying the Simplex-Centroid Mixture Design. <b>Methods:</b> Eleven solvents were tested for their affinities with <i>A. pyrethrum</i> bioactive molecules, and the three best solvents (water, methanol, and ethanol) were subjected to mixing modeling for optimization, and different models were developed to study the binary and ternary combined effects. Extract fractionation was performed using four solvents namely Chloroform, dichloromethane, ethyl acetate, and hexane. The evaluation of the antioxidant activity of the fractions was performed using various tests (TAC, DPPH, FRAP, ABTS, chelating power, and nitric oxide scavenging activity). <b>Results: </b>The developed models and all their parameters were significant. The analysis of the response surfaces generated by the models indicated that the quantity of phenolic compounds extracted in the binary mixtures and the total antioxidant activity of the extracts increase with the increasing water percent in the solvent mixture using both ethanol or methanol. While, the binary &ldquo;water-ethanol&rdquo;, and &ldquo;water-methanol&rdquo; and the ternary mixture proved to be the most effective combinations for extracting anti-radical compounds. Fractionation results showed that the aqueous fraction exhibited the highest antioxidant activity, due to its higher content of phenolic compounds. <b>Conclusion: </b></span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">With this type of research, it would be easier to treat and prevent human damage from free radicals and also replace synthetic antioxidants in industry.</span></span></div> Anacyclus pyrethrum var. depressus (Ball) Maire, Antioxidant capacity, Phenolic compounds, Simplex-Centroid Mixture Design, Solvent optimization 31 51 http://jmp.ir/browse.php?a_code=A-10-6807-1&slc_lang=en&sid=1 Oumaima Chater اومیما چاتر oumaima.chater@usmba.ac.ma 100319475328460047757 100319475328460047757 Yes University of Sidi Mohamed Ben Abdellah, Faculty of Science and Technology, Laboratory of Functional Ecology and Environmental Engineering, Fez, Morocco دانشگاه سیدی محمد بن عبدالله، دانشکده علوم و فناوری، آزمایشگاه اکولوژی کاربردی و مهندسی محیط زیست، فاس، مراکش Smail Aazza اسماعیل اعزا smail.aazza@gmail.com 100319475328460047758 100319475328460047758 No Mohammed 1st University, Oujda, Nador Multidisciplinary Faculty (FPN), OLMANBGPE, Nador, Morocco دانشگاه محمد اول، اوجدا، دانشکده چند رشته‎ای نادور (FPN)، OLMANBGPE، نادور، مراکش Hiba Bouzaid هیبه بوزید hiba.bouzaid@usmba.ac.ma 100319475328460047759 100319475328460047759 No University of Sidi Mohamed Ben Abdellah, Faculty of Science and Technology, Laboratory of Organic Chemistry, Fez, Morocco دانشگاه سیدی محمد بن عبدالله، دانشکده علوم و فناوری، آزمایشگاه شیمی آلی، فاس، مراکش Lahsen El Ghadraoui لاحسن ال قدراوی lahsen.elghadraoui@usmba.ac.ma 100319475328460047760 100319475328460047760 No University of Sidi Mohamed Ben Abdellah, Faculty of Science and Technology, Laboratory of Functional Ecology and Environmental Engineering, Fez, Morocco دانشگاه سیدی محمد بن عبدالله، دانشکده علوم و فناوری، آزمایشگاه اکولوژی کاربردی و مهندسی محیط زیست، فاس، مراکش