en Antidermatophyte Activity of the Essential oil of <i>Hypericum perforatum</i> of North of Iran فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Dermatophytes are the main cause of human superficial mycosis that is still a public health problem especially in tropical countries such as Iran. <strong>Objective:</strong> The aim of this study was determining the antifungal effect of <em>Hypercom perforatum</em> essential oil. <strong>Methods: </strong>The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for the essential oil of the plant <em>Hypericum perforatum</em> against various dermatophytes were determined. The essential oil of <em>Hypericum perforatum</em> was obtained by hydro distillation of the dried plant. Clinical isolates of dermatophytes (<em>Epidermophyton floccosum</em>, <em>Microsporum canis</em>, <em>Microsporum gypseum</em>, <em>T. mentagrophytes</em> var. interdigital, <em>T. mentagrophytes</em> var. <em>mentagrophytes.</em>, <em>T. rubrum</em> and <em>Trichophyton tonsurans</em>) were used for determining antifungal activity of this essential oil by <em>in vitro</em> tube dilution technique. <strong>Results:</strong> MIC90 and MFC90 values were remarkable. <em>T. mentagrophytes</em> var. interdigital showed a>1 log10 difference in viable count between treatment and control within the first hour, whereas <em>E. floccosum</em> did not. The essential oil of <em>H. perforatum</em> sufficiently inhibited and killed all tested dermatophytes in all different dilutions. The changes in growth curve of the treated dermatophytes were significant compared with the untreated dermatophytes. <strong>Conclusion: </strong>Terpinen-4-ol is the main component of the essential oil of <em>H. perforatum</em>, and perhaps could play the important role in antidermatophyte activity among the other components. It is suggested trying the in-vivo effects of <em>Hypericum perforatum</em> ointment or its other medicinal forms in the treatment and controlling of dermatophytes infections.</div> <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Dermatophytes are the main cause of human superficial mycosis that is still a public health problem especially in tropical countries such as Iran. <strong>Objective:</strong> The aim of this study was determining the antifungal effect of <em>Hypercom perforatum</em> essential oil. <strong>Methods: </strong>The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for the essential oil of the plant <em>Hypericum perforatum</em> against various dermatophytes were determined. The essential oil of <em>Hypericum perforatum</em> was obtained by hydro distillation of the dried plant. Clinical isolates of dermatophytes (<em>Epidermophyton floccosum</em>, <em>Microsporum canis</em>, <em>Microsporum gypseum</em>, <em>T. mentagrophytes</em> var. interdigital, <em>T. mentagrophytes</em> var. <em>mentagrophytes.</em>, <em>T. rubrum</em> and <em>Trichophyton tonsurans</em>) were used for determining antifungal activity of this essential oil by <em>in vitro</em> tube dilution technique. <strong>Results:</strong> MIC90 and MFC90 values were remarkable. <em>T. mentagrophytes</em> var. interdigital showed a>1 log10 difference in viable count between treatment and control within the first hour, whereas <em>E. floccosum</em> did not. The essential oil of <em>H. perforatum</em> sufficiently inhibited and killed all tested dermatophytes in all different dilutions. The changes in growth curve of the treated dermatophytes were significant compared with the untreated dermatophytes. <strong>Conclusion: </strong>Terpinen-4-ol is the main component of the essential oil of <em>H. perforatum</em>, and perhaps could play the important role in antidermatophyte activity among the other components. It is suggested trying the in-vivo effects of <em>Hypericum perforatum</em> ointment or its other medicinal forms in the treatment and controlling of dermatophytes infections.</div> <,i>,Hypericum perforatum<,/i>,, Antidermatophyte activity <,i>,Hypericum perforatum<,/i>,, Antidermatophyte activity 110 117 http://jmp.ir/browse.php?a_code=A-10-200-2&slc_lang=en&sid=1 M Larypoor M Larypoor 100319475328460038302 100319475328460038302 No Department of veterinary Mycology, Faculty of veterinary, Science and research branch Azad Islamic university Department of veterinary Mycology, Faculty of veterinary, Science and research branch Azad Islamic university A Akhavansepahy A Akhavansepahy 100319475328460038303 100319475328460038303 No Department of Microbiology, North Branch of Islamic Azad University Department of Microbiology, North Branch of Islamic Azad University N Rahimifard N Rahimifard rahimif@fdo.ir 100319475328460038304 100319475328460038304 Yes Food and Drug Control Laboratories and Food and Drug Laboratory Research Center, MOH Food and Drug Control Laboratories and Food and Drug Laboratory Research Center, MOH H Rashedi H Rashedi 100319475328460038305 100319475328460038305 No Department of Biotechnology, School of Engineering, College of Engineering, University of Tehran (UT), Department of Biotechnology, School of Engineering, College of Engineering, University of Tehran (UT),