fa Antihistaminic and Anticholinergic Activity of Methanolic Extract of Barberry Fruit (<i>Berberis vulgaris</i>) in the Guinea- Pig Ileum فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Barberry (<em>Berberris vulgaris</em>) is a well known medicinal plant in Iran and has also been used as food. <strong>Objective:</strong> This study was conducted to evaluate antihistaminic and anticholinergic activity of methanolic extract of barberry fruit. <strong>Methods:</strong> Methanolic extract was prepared and pharmacologically studied on isolated guinea- pig ileum, dose- response curves of histamine and acetylcholine with and without methanolic extract were plotted. <strong>Results:</strong> The pA2 values for antihistaminic activity of methanolic extract and dexchlorpheniramine were calculated (extract pA2&plusmn; S.E.M = 3.53 &plusmn; 0.16 [-logC(g/l)] dexchlorpheniramine pA2 &plusmn; S.E.M.= 9.36 &plusmn; 0.14 ([-logC (M)]) and compared with each other. The pA2 values of anticholinergic activity of methanolic extract and atropine were also calculated (extract pA2&plusmn; S.E.M = 4.18 &plusmn; 0.17 [-logC(g/)] atropine, PA2 +S.E.M = 8.99 &plusmn; 0.13 [-logC(M)]) and compared. <strong>Conclusion: </strong>The results indicated antihistaminic and anticholinergic activity of methanolic extract.</div> <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Barberry (<em>Berberris vulgaris</em>) is a well known medicinal plant in Iran and has also been used as food. <strong>Objective:</strong> This study was conducted to evaluate antihistaminic and anticholinergic activity of methanolic extract of barberry fruit. <strong>Methods:</strong> Methanolic extract was prepared and pharmacologically studied on isolated guinea- pig ileum, dose- response curves of histamine and acetylcholine with and without methanolic extract were plotted. <strong>Results:</strong> The pA2 values for antihistaminic activity of methanolic extract and dexchlorpheniramine were calculated (extract pA2&plusmn; S.E.M = 3.53 &plusmn; 0.16 [-logC(g/l)] dexchlorpheniramine pA2 &plusmn; S.E.M.= 9.36 &plusmn; 0.14 ([-logC (M)]) and compared with each other. The pA2 values of anticholinergic activity of methanolic extract and atropine were also calculated (extract pA2&plusmn; S.E.M = 4.18 &plusmn; 0.17 [-logC(g/)] atropine, PA2 +S.E.M = 8.99 &plusmn; 0.13 [-logC(M)]) and compared. <strong>Conclusion: </strong>The results indicated antihistaminic and anticholinergic activity of methanolic extract.</div> Barberry, Antihistaminc, Anticholinergic, Guinea-Pig ileum, Methanolic extract Barberry, Antihistaminc, Anticholinergic, Guinea-Pig ileum, Methanolic extract 99 105 http://jmp.ir/browse.php?a_code=A-10-197-1&slc_lang=fa&sid=1 R Khosrokhavar R Khosrokhavar khosrokhavar_r@yahoo.com 100319475328460037850 100319475328460037850 Yes Food and Drug Laboratory Research Center and Food and Drug Control Laboratories, MOH& ME Food and Drug Laboratory Research Center and Food and Drug Control Laboratories, MOH& ME A Ahmadiani A Ahmadiani 100319475328460037851 100319475328460037851 No Department of Pharmacology, Faculty of Medicine, the Medical Sciences University of Shaheed Beheshti Department of Pharmacology, Faculty of Medicine, the Medical Sciences University of Shaheed Beheshti F Shamsa F Shamsa 100319475328460037852 100319475328460037852 No Department of Chemistry, Faculty of Pharmacy, the Medical Sciences University of Tehran Department of Chemistry, Faculty of Pharmacy, the Medical Sciences University of Tehran