en Study the Effect of Silymarin and Vitamin C in Skin Aging Induced by UVB Rays on the Mice Skin Redox System فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Exposure of the skin against occupational hazards is growing every day. Many scientists are trying to identify aspects about the mechanisms of damages done to the skin by them. The skin is the second metabolism organ of the body where redox-dependent metabolic pathways overcome. By considering the UV index, Iranians are at high risk of UVB damage by generation of reactive oxygen species (ROS) that leads to skin aging.<br> <strong>Objective:</strong> In this study, we discuss the effects of the UVB rays on redox system; evaluate and compare the protective role of Silymarin and Vitamin C against UVB-induced skin aging.<br> <strong>Method:</strong> Male Balb/C mice were divided into 6 groups of 6 mices: Silymarin, Vitamin C, Control, UVB exposed, UVB exposed treated with Silymarin and UVB exposed treated with Vitamin C. The inhibitory effect of Silymarin and Vitamin C for skin aging were determined by wrinkle formation, epidermal thickness and dermal inflammation. The anti-photo aging effects of Silymarin and Vitamin C have been assessed by SOD, GSH, GSH_PX and TAC kits.<br> <strong>Results:</strong> The flake in UVB irradiated groups was significantly different (P<0.05). The mean length of the wrinkle in UVB irradiated group very significantly increased (P<0.01) and the collagen reduction, increased in very high significant different in aged control group (P<0.001). The mean TAC in VitaminC group increased very significantly high. (P <0.0001)<br> <strong>Conclusion:</strong> The results indicate that the Silymarin and Vitamin C have anti-photo aging effects suggested to use in anti-wrinkle, anti erythematosus, anti-inflammatory topical cosmetics products.</div> <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Exposure of the skin against occupational hazards is growing every day. Many scientists are trying to identify aspects about the mechanisms of damages done to the skin by them. The skin is the second metabolism organ of the body where redox-dependent metabolic pathways overcome. By considering the UV index, Iranians are at high risk of UVB damage by generation of reactive oxygen species (ROS) that leads to skin aging.<br> <strong>Objective:</strong> In this study, we discuss the effects of the UVB rays on redox system; evaluate and compare the protective role of Silymarin and Vitamin C against UVB-induced skin aging.<br> <strong>Method:</strong> Male Balb/C mice were divided into 6 groups of 6 mices: Silymarin, Vitamin C, Control, UVB exposed, UVB exposed treated with Silymarin and UVB exposed treated with Vitamin C. The inhibitory effect of Silymarin and Vitamin C for skin aging were determined by wrinkle formation, epidermal thickness and dermal inflammation. The anti-photo aging effects of Silymarin and Vitamin C have been assessed by SOD, GSH, GSH_PX and TAC kits.<br> <strong>Results:</strong> The flake in UVB irradiated groups was significantly different (P<0.05). The mean length of the wrinkle in UVB irradiated group very significantly increased (P<0.01) and the collagen reduction, increased in very high significant different in aged control group (P<0.001). The mean TAC in VitaminC group increased very significantly high. (P <0.0001)<br> <strong>Conclusion:</strong> The results indicate that the Silymarin and Vitamin C have anti-photo aging effects suggested to use in anti-wrinkle, anti erythematosus, anti-inflammatory topical cosmetics products.</div> ,Redox, Silymarin, Skin aging, Ultraviolet B (UVB), Vitamin C Abbreviations: ROS (reactive oxygen species), GSH (glutathione), TAC (total antioxidant capacity), SOD (super oxide dismutase), GSH_PX (glutathione peroxidase), UV (ultraviolet ray) Redox, Silymarin, Skin aging, Ultraviolet B (UVB), Vitamin C, Abbreviations: ROS (reactive oxygen species), GSH (glutathione), TAC (total antioxidant capacity), SOD (super oxide dismutase), GSH_PX (glutathione peroxidase), UV (ultraviolet ray) 130 144 http://jmp.ir/browse.php?a_code=A-10-958-6&slc_lang=en&sid=1 HR Ahmadi Ashtiani HR Ahmadi Ashtiani ahmadi@iaups.ac.ir 100319475328460035105 100319475328460035105 Yes Department of Basic Sciences, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran گروه علوم پایه ، دانشکده داروسازی ، دانشگاه علوم پزشکی تهران ، دانشگاه آزاد اسلامی ، تهران ، ایران H Rastegar H Rastegar 100319475328460035106 100319475328460035106 No Cosmetic Prouducts Research Center, Tehran, Iran مرکز تحقیقات سازمان غذا و دارو، وزارت بهداشت، درمان و آموزش پزشکی، تهران، ایران AA Salarian AA Salarian 100319475328460035107 100319475328460035107 No Assistant Professor of Toxin Research Center, AJA University of Medical Science, Tehran, Iran استادیار مرکز تحقیقات توکسین ، دانشگاه علوم پزشکی آجا ، تهران ، ایران F Rahmani F Rahmani 100319475328460035108 100319475328460035108 No Department of Basic Sciences, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran گروه علوم پایه ، دانشکده داروسازی ، دانشگاه علوم پزشکی تهران ، دانشگاه آزاد اسلامی ، تهران ، ایران Sh Rezazadeh Sh Rezazadeh 100319475328460035109 100319475328460035109 No Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran مرکز تحقیقات گیاهان دارویی ، پژوهشکده گیاهان دارویی ، ACECR ، کرج ، ایران H Sedghi Zadeh H Sedghi Zadeh 100319475328460035110 100319475328460035110 No Professor of Pharmacy Department of Life Sciences and Biotechnologies, University of Ferrara, Italy استاد گروه داروسازی علوم زندگی و زیست فناوری ، دانشگاه فرارا ، ایتالیا