en Effects of Gamma Irradiated and Non-Irradiated Sewage Sludge on Essential Oil Content and Constituents of <i>Ocimum basilicum</i> L. كشاورزی و اتنوبوتانی Agriculture & Ethnobotany پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> The increasing amount of sewage sludge (SS) and more legislative regulation of its disposal have stimulated the need for developing new technologies including gamma irradiation to recycle SS efficiently.<br> <strong>Objective:</strong> To evaluate the effects of gamma irradiated and non-irradiated SS (SSGI and SSNI, respectively) on essential oil (EO) content and composition of <em>Ocimum</em> <em>basilicum</em> L.<br> <strong>Methods:</strong> A greenhouse experiment was conducted with 15, 30, and 60 g kg^-1 of SS irradiated with absorbed doses of 5, 10, and 20 kGy gamma-ray and soil without SS and irradiation as the control treatment. The essential oil was isolated by hydro-distillation and analyzed by Gas chromatography and Gas chromatography-mass spectrometry techniques.<br> <strong>Results:</strong> The applications of SSGI and SSNI at all levels except 15 g kg^-1 significantly enhanced EO content compared to the control. The using of 60 g kg^-1 SS in each absorbed dose significantly reduced EO yield relative to the control, while it was significantly increased under 15 and 30 g kg^-1 SSNI and SSGI. Also, in 60 g SS kg^-1, the restriction effects on EO yield were mitigated with increasing the absorbed dose of gamma-ray, but it was insignificant with increasing absorbed dose to 10 and 20 relative to 5 kGy<span dir="RTL">.</span> The methyl chavicol as a major constituent of EO was significantly increased in SSGI and SSNI as compared with the control. However, the increment in the methyl chavicol content was not significant between the treatments &ge; 30 g kg^-1SSGI or SSNI and 15 g kg^-1SS at absorbed dose of &ge;10 kGy.</div> <div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> To improve essential oil content and composition, application of &le; 30 g kg^-1 sewage sludge with 5 kGy absorbed dose of gamma-ray can be suggested at similar conditions.</div> <strong>Background:</strong><strong> The increasing amount of sewage sludge (SS) and more legislative regulation of its disposal have stimulated the need for developing new technologies including gamma irradiation to recycle SS efficiently. </strong><br> <strong>Objective:</strong><strong> To evaluate the effects of gamma irradiated and non-irradiated SS (SSGI and SSNI, respectively) on essential oil (EO) content and composition of <em>Ocimum</em> <em>basilicum</em> L.</strong><br> <strong>Methods:</strong><strong> A greenhouse experiment was conducted with 15, 30, and 60 g kg^-1 of SS irradiated with absorbed doses of 5, 10, and 20 kGy</strong> <strong>gamma-ray and soil without SS and irradiation as the control treatment. The essential oil was isolated by hydro-distillation and analyzed by Gas chromatography and Gas chromatography-mass spectrometry techniques.</strong><br> <strong>Results:</strong><strong> The applications of SSGI and SSNI at all levels except 15 g kg^-1 significantly enhanced EO content compared to the control. The using of 60 g kg^-1 SS in each absorbed dose significantly reduced EO yield relative to the control, while it was significantly increased under 15 and 30 g kg^-1 SSNI and SSGI. </strong><strong>Also, in 60 g SS kg^-1, </strong><strong>the restriction effects on EO yield were mitigated with increasing the absorbed dose of gamma-ray, but it was insignificant with increasing absorbed dose to 10 and 20 relative to 5 kGy<span dir="RTL">.</span> The methyl chavicol as a major constituent of EO was significantly increased in SSGI and SSNI as compared with the control. However, the increment in the methyl chavicol content was not significant between the treatments &ge; 30 g kg^-1SSGI or SSNI and 15 g kg^-1SS at absorbed dose of &ge;10 kGy. </strong> <table cellpadding="0" cellspacing="0" width="100%"> <tbody> <tr> <td> <div><strong>98</strong></div> </td> </tr> </tbody> </table> <strong>Conclusion: To improve essential oil content and composition, application of &le; 30 g kg^-1 sewage sludge with 5 kGy absorbed dose of gamma-ray can be suggested at similar conditions.</strong> Basil, Essential oil, Gamma irradiation, Medicinal plant, Sewage sludge Basil, Essential oil, Gamma irradiation, Medicinal plant, Sewage sludge 99 117 http://jmp.ir/browse.php?a_code=A-10-2100-2&slc_lang=en&sid=1 B Asgari Lajayer B Asgari Lajayer 100319475328460035098 100319475328460035098 No Department of Soil Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Department of Soil Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran NA Najafi NA Najafi n-najafi@tabrizu.ac.ir 100319475328460035099 100319475328460035099 Yes Department of Soil Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Department of Soil Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran E Moghiseh E Moghiseh 100319475328460035100 100319475328460035100 No Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran M Mosaferi M Mosaferi 100319475328460035101 100319475328460035101 No Health and Environment Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Health and Environment Research Center, Tabriz University of Medical Sciences, Tabriz, Iran J Hadian J Hadian 100319475328460035102 100319475328460035102 No Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran