en Alert for Consumption of <i>Dorema aucheri</i>: an Edible Medicinal Plant of Iran فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> <em>Dorema aucheri </em>(Apiaceae) grows in west and southwest of Iran. Young leaves of this plant are used orally as pickle or for cooking some local foods, as well as for the treatment of parasitic digestive infections and constipation.<br> <strong>Objective: </strong>The aim of present study was evaluation of cytotoxic potential and total phenols determination of <em>D. aucheri </em>young aerial parts extract and fractions and its pickle extract.<br> <strong>Method: </strong>The dried powders of <em>D. aucheri </em>young aerial parts and pickles were macerated with 80% methanol and fractionated with petroleum ether (PE), chloroform (CL), ethyl acetate (EA) and methanol (ME). Cytotoxic activity of the total extract, fractions and pickle extract on HT-29, MDA-MB-231, A549, Hela cancer and normal fibroblast cell lines were assessed by MTT assay. Total phenol contents of all samples were determined by Folin-Ciocalteu method.<br> <strong>Results:</strong> The potent cytotoxic activities were shown by PE and CL fractions on normal fibroblast cell line with IC₅₀ equal to 17.66<span dir="RTL">&plusmn;</span>2.3 and 48.80<span dir="RTL">&plusmn;</span>1.24 &micro;g/ml, respectively. Other fractions and extracts didn&rsquo;t have considerable cytotoxic effects on other cell lines. In addition, two mentioen fractions had the lowest total phenols. ME and EA fractions with highest amounts of phenols showed the lowest cytotoxicity.<br> <strong>Conclusion: </strong>It is concluded <em>D. aucheri</em> compounds have potential to exhibit toxic effects on healthy cells. On the other hand, there is negative correlation between cytotoxicity and phenol contents of <em>D. aucheri</em> fractions.</div> <div style="text-align: justify;"><strong>Background:</strong> <em>Dorema aucheri </em>(Apiaceae) grows in west and southwest of Iran. Young leaves of this plant are used orally as pickle or for cooking some local foods, as well as for the treatment of parasitic digestive infections and constipation.<br> <strong>Objective:</strong> The aim of present study was evaluation of cytotoxic potential and total phenols determination of <em>D. aucheri </em>young aerial parts extract and fractions and its pickle extract.<br> <strong>Method:</strong> The dried powders of <em>D. aucheri </em>young aerial parts and pickles were macerated with 80% methanol and fractionated with petroleum ether (PE), chloroform (CL), ethyl acetate (EA) and methanol (ME). Cytotoxic activity of the total extract, fractions and pickle extract on HT-29, MDA-MB-231, A549, Hela cancer and normal fibroblast cell lines were assessed by MTT assay. Total phenol contents of all samples were determined by Folin-Ciocalteu method.<br> <strong>Results: </strong>The potent cytotoxic activities were shown by PE and CL fractions on normal fibroblast cell line with IC₅₀ equal to 17.66<span dir="RTL">&plusmn;</span>2.3 and 48.80<span dir="RTL">&plusmn;</span>1.24 &micro;g/ml, respectively. Other fractions and extracts didn&rsquo;t have considerable cytotoxic effects on other cell lines. In addition, two mentioen fractions had the lowest total phenols. ME and EA fractions with highest amounts of phenols showed the lowest cytotoxicity.<br> <strong>Conclusion: </strong>It is concluded <em>D. aucheri</em> compounds have potential to exhibit toxic effects on healthy cells. On the other hand, there is negative correlation between cytotoxicity and phenol contents of <em>D. aucheri</em> fractions.</div> Bilhar, Cytotoxic, MTT assay, Phenolics, Umbelliferae Bilhar, Cytotoxic, MTT assay, Phenolics, Umbelliferae 77 84 http://jmp.ir/browse.php?a_code=A-10-2063-2&slc_lang=en&sid=1 M Eftekhari M Eftekhari 100319475328460035088 100319475328460035088 No School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran F Oskou F Oskou 100319475328460035089 100319475328460035089 No School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran Z Tofighi Z Tofighi 100319475328460035090 100319475328460035090 No Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, 14174-14411, Tehran, Iran Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, 14174-14411, Tehran, Iran E Motevaseli E Motevaseli e_motevaseli@tums.ac.ir 100319475328460035091 100319475328460035091 Yes Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran S Jafari Nodooshan S S Jafari Nodooshan S 100319475328460035092 100319475328460035092 No Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran MR Shams Ardekani MR Shams Ardekani 100319475328460035093 100319475328460035093 No School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran