en Effects of Nano Elicitors on Callus Induction and Mucilage Production in Tissue Culture of <i>Linum usitatissimum</i> L. بيوتكنولوژی Biotechnology پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Tissue culture is a new approach for production of secondary metabolites with many advantages over conventional methods. Elicitors such as nano particles are molecules that can stimulate the production of secondary metabolites.<br> <strong>Objective:</strong> This study was conducted to evaluate the effects of some nano particles on mucilage production in <em>Linum usitatissimum</em> under tissue culture conditions.<br> <strong>Methods:</strong> Concentrations of 5, 10 and 20 mg/L of nano-ZnO, nano-SiO₂ and nano-Al₂O₃ were selected as elicitor treatments. The cultures incubated in growth room at 25 &plusmn; 1 ^oC with 16/8 h illumination periods. After 2 weeks of incubation callus fresh weight, percentage of mucilage and mucilage yield were recorded.<br> <strong>Results:</strong> The results indicated the positive effect of low dosage of nano elicitors on callus induction and mucilage production. Furthermore, the callus induction is tightly dependent to the presence of auxin and cytokinin, which stimulate both cell division and cell elongation.<br> <strong>Conclusion:</strong> Generally, the type and concentration of nano elicitors had significant effects on the mucilage content.<br> &nbsp;</div> <div dir="ltr" style="text-align: justify;">&nbsp;</div> <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Tissue culture is a new approach for production of secondary metabolites with many advantages over conventional methods. Elicitors such as nano particles are molecules that can stimulate the production of secondary metabolites.<br> <strong>Objective:</strong> This study was conducted to evaluate the effects of some nano particles on mucilage production in <em>Linum usitatissimum</em> under tissue culture conditions.<br> <strong>Methods:</strong> Concentrations of 5, 10 and 20 mg/L of nano-ZnO, nano-SiO₂ and nano-Al₂O₃ were selected as elicitor treatments. The cultures incubated in growth room at 25 &plusmn; 1 ^oC with 16/8 h illumination periods. After 2 weeks of incubation callus fresh weight, percentage of mucilage and mucilage yield were recorded.<br> <strong>Results:</strong> The results indicated the positive effect of low dosage of nano elicitors on callus induction and mucilage production. Furthermore, the callus induction is tightly dependent to the presence of auxin and cytokinin, which stimulate both cell division and cell elongation.<br> <strong>Conclusion:</strong> Generally, the type and concentration of nano elicitors had significant effects on the mucilage content.<br> &nbsp;</div> <div dir="ltr" style="text-align: justify;">&nbsp;</div> Linum usitatissimum L., Callus, Elicitor, Mucilage, Nano particle, Tissue culture Linum usitatissimum L., Callus, Elicitor, Mucilage, Nano particle, Tissue culture 45 54 http://jmp.ir/browse.php?a_code=A-10-44-113&slc_lang=en&sid=1 S Kavianifar S Kavianifar naghdibadi@yahoo.com 100319475328460035292 100319475328460035292 No Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran K Ghodrati K Ghodrati naghdibadi@yahoo.com 100319475328460035293 100319475328460035293 No Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran H Naghdi Badi HA Naghdi Badi naghdibadi@yahoo.com 100319475328460035294 100319475328460035294 No Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran AR Etminan AR Etminan alietminan55@yahoo.com 100319475328460035295 100319475328460035295 Yes Department of Biotechnology and Plant breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran Department of Biotechnology and Plant breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran