Journal of Medicinal Plants
فصلنامه گياهان دارویی
J. Med. Plants
Medical Sciences
http://jmp.ir
1
admin
2717-204X
2717-2058
10.61186/jmp
14
8888
13
en
jalali
1390
6
1
gregorian
2011
9
1
10
39
online
1
fulltext
fa
Effect of Ethanolic Extract of <i>Mespilus germanica</i> on Cutaneous Leishmaniasis in BALB/c Mice
Effect of Ethanolic Extract of <i>Mespilus germanica</i> on Cutaneous Leishmaniasis in BALB/c Mice
فارماكولوژی و سم شناسی
Pharmacology & Toxicology
پژوهشی
Research
<div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Leishmaniasis is a vector-borne disease caused by flagellated protozoan parasites of the genus <em>Leishmania</em>, which affects both humans and other mammals. Most of the available drugs against the disease are toxic and some are parasite resistance to them. <strong>Objective:</strong> The purpose of this study was to examine the effect of <em>Mespilus germanica</em> extract on cutaneous leishmaniasis (CL) in BALB/c Mice. <strong>Methods:</strong> Ethanolic extract of Mespilus germanica with 40, 60 and 80% concentrations were prepared. Then, the BALB/c mice were inoculated subcutaneously by 0.1 ml liquid phase culture containing promastigotes of <em>Leishmania major</em>. Ethanolic extract of the leaves of <em>Mespilus germanica</em> in different concentrations, were used topically on CL lesions. <strong>Results:</strong> The mean diameter of the lesions were decreased, and also the number of parasites in the lesions had declined with complete healing by ending the period time of treatment in 4 mice (26.7%), (p< 0.05) and in 9 animals (82%), (p<0.05) respectively, by using the 40% concentration of the extract. Also in a concentration of 60%, mean ulcer diameter decreased, with complete healing in 3 mice (20%), (p<0.001). In this concentration, the mean number of parasites in lesions had declined (66.4%), with total elimination in 8 animals (p< 0.001). <strong>Conclusion:</strong> We showed that the extract of <em>Mespilus germanica</em> has the highest effectiveness in concentration of 40%, causing greater reductions in both ulcer diameter and the number of parasites in the lesions compared with other prepared concentrations. Therefore, we suggest the use of 40% extract for the treatment of human cases.</div>
<div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Leishmaniasis is a vector-borne disease caused by flagellated protozoan parasites of the genus <em>Leishmania</em>, which affects both humans and other mammals. Most of the available drugs against the disease are toxic and some are parasite resistance to them. <strong>Objective:</strong> The purpose of this study was to examine the effect of <em>Mespilus germanica</em> extract on cutaneous leishmaniasis (CL) in BALB/c Mice. <strong>Methods:</strong> Ethanolic extract of Mespilus germanica with 40, 60 and 80% concentrations were prepared. Then, the BALB/c mice were inoculated subcutaneously by 0.1 ml liquid phase culture containing promastigotes of <em>Leishmania major</em>. Ethanolic extract of the leaves of <em>Mespilus germanica</em> in different concentrations, were used topically on CL lesions. <strong>Results:</strong> The mean diameter of the lesions were decreased, and also the number of parasites in the lesions had declined with complete healing by ending the period time of treatment in 4 mice (26.7%), (p< 0.05) and in 9 animals (82%), (p<0.05) respectively, by using the 40% concentration of the extract. Also in a concentration of 60%, mean ulcer diameter decreased, with complete healing in 3 mice (20%), (p<0.001). In this concentration, the mean number of parasites in lesions had declined (66.4%), with total elimination in 8 animals (p< 0.001). <strong>Conclusion:</strong> We showed that the extract of <em>Mespilus germanica</em> has the highest effectiveness in concentration of 40%, causing greater reductions in both ulcer diameter and the number of parasites in the lesions compared with other prepared concentrations. Therefore, we suggest the use of 40% extract for the treatment of human cases.</div>
Cutaneous leishmaniasis, Mespilus germanica, Extract, BALB/c mice
Cutaneous leishmaniasis, Mespilus germanica, Extract, BALB/c mice
76
81
http://jmp.ir/browse.php?a_code=A-10-147-2&slc_lang=fa&sid=1
N
Shariatifar
N
Shariatifar
n_shariatifar@yahoo.com
100319475328460037499
100319475328460037499
Yes
Food and Drug Deputy, Tehran University of Medical Sciences
Food and Drug Deputy, Tehran University of Medical Sciences
R
Rahimnia
R
Rahimnia
100319475328460037500
100319475328460037500
No
Graduate, College of Medical, Tehran University of Medical Sciences
Graduate, College of Medical, Tehran University of Medical Sciences
AM
Jamshidi
AM
Jamshidi
100319475328460037501
100319475328460037501
No
Food and Drug Deputy, Ministry of Health and Medical Education
Food and Drug Deputy, Ministry of Health and Medical Education
M
Pirali Hamedani
M
Pirali Hamedani
100319475328460037502
100319475328460037502
No
Medicinal Chemistry Department, College of Pharmacy, Tehran University of Medical Sciences
Medicinal Chemistry Department, College of Pharmacy, Tehran University of Medical Sciences
Sh
Shoeibi
Sh
Shoeibi
100319475328460037503
100319475328460037503
No
Food and Drug Laboratory Research Center (FDLRC), Ministry of Health (MOH)
Food and Drug Laboratory Research Center (FDLRC), Ministry of Health (MOH)