en Phytochemical Evaluation and Plant Regeneration Through Somatic Embryogenesis of <i>Ferulago angulata</i> subsp. <i>Carduchorum</i> (Boiss. and Hausskn), an Endangered Medicinal Plant بيوتكنولوژی Biotechnology پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong><em> Ferulago angulata</em> is an important medicinal plant and endemic to Iran which is an endangered Plant.<br> <strong>Objective:</strong> The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat.<br> <strong>Metthods:</strong> Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was analyzed by the GC and GC-MS. For callus induction, explants were cultured on MS medium supplemented with NAA, 2,4-D and BA. For somatic embryogenesis, the calli were cultured on MS medium supplemented with full, 1/4 and 1/10 fold of growth regulators and MS medium without growth regulator. For maturation, embryogenic clumps were transferred on MS medium supplemented with BA, 2,4-D and 30 or 60 g l^-1 sucrose. The small embryogenic clumps were transferred to medium with GA3.<br> <strong>Results:</strong> The main components of essential oils were Ocimene (29.84%), followed by &alpha;-pinene (27.01%), Isobornyl formate (7.7%) and gamma-Terpinene (4.85%). The highest percentage of callus induction observed in the root and hypocotyl explants. The maximum somatic embryogenesis was obtained in MS medium with 0.5 mg l^-1 2, 4-D via hypocotyls derived explants. The highest frequency of maturation was obtained in MS medium containing 30 g l^-1sucrose without growth regulators.<br> <strong>Conclution:</strong> This plant had a noticeable amount of essential oil with valuable ingredients and tissue culture techniques could be applied for its mass propagation, conservation of germplasm and <em>in vitro</em> regeneration.</div> <div style="text-align: justify;"><strong>Background:</strong><em> Ferulago angulata</em> is an important medicinal plant and endemic to Iran which is an endangered Plant.<br> <strong>Objective:</strong> The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat.<br> <strong>Metthods:</strong> Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was analyzed by the GC and GC-MS. For callus induction, explants were cultured on MS medium supplemented with NAA, 2,4-D and BA. For somatic embryogenesis, the calli were cultured on MS medium supplemented with full, 1/4 and 1/10 fold of growth regulators and MS medium without growth regulator.<a name="OLE_LINK64"></a> For maturation, embryogenic clumps were transferred on MS medium supplemented with BA, 2,4-D and 30 or 60 g l^-1 sucrose. The small embryogenic clumps were transferred to medium with GA3.<br> <strong>Results:</strong> The main components of essential oils were Ocimene (29.84%), followed by &alpha;-pinene (27.01%), Isobornyl formate (7.7%) and gamma-Terpinene (4.85%). The highest percentage of callus induction observed in the root and hypocotyl explants. The maximum somatic embryogenesis was obtained in MS medium with 0.5 mg l^-1 2, 4-D via hypocotyls derived explants. The highest frequency of maturation was obtained in MS medium containing 30 g l^-1sucrose without growth regulators.<br> <strong>Conclution:</strong> This plant had a noticeable amount of essential oil with valuable ingredients and tissue culture techniques could be applied for its mass propagation, conservation of germplasm and <em>in vitro</em> regeneration.</div> ,Ferulago angulata, Callus induction, Embryogenesis, Essential oil Ferulago angulata, Callus induction, Embryogenesis, Essential oil 92 107 http://jmp.ir/browse.php?a_code=A-10-75-7&slc_lang=en&sid=1 HA Naghdi Badi حسنعلی نقدی بادی Naghdibadi@yahoo.com 100319475328460041319 100319475328460041319 Yes Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران Sh Salek Hashemi شهناز سالک هاشمی salekshahnaz@yahoo.com 100319475328460041320 100319475328460041320 No Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran دانشجوی کارشناسی ارشد، گروه بیوتکنولوژی و اصلاح نباتات ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ، ایران AA Nasrollahnezhad Qomi علی‌اصغر نصرالله نژاد قمی Ali1346nn@yahoo.com 100319475328460041321 100319475328460041321 No Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran عضو هیئت علمی گروه بیوتکنولوژی و اصلاح نباتات ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ، ایران Saeed Navabpour سعید نواب‌پور S.navabpour@yahoo.com 100319475328460041322 100319475328460041322 No Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran عضو هیئت علمی گروه بیوتکنولوژی و اصلاح نباتات ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ، ایران A Qaderi اردشیر قادری Ardeshir582008@gmail.com 100319475328460041323 100319475328460041323 No Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران