en Total Phenol, Antioxidant and Antibacterial Activity of the Essential Oil and Extracts of <i>Ferulago angulata</i> ssp. <i>angulata</i> فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div dir="ltr" style="text-align: justify;">&nbsp;<strong>Background:</strong>&nbsp;In recent years, plants natural products have gained considerable attention as natural preservers for use in different industries. Due to their free radical scavenging activities, phenolic compounds can prevent a wide range of diseases such as cancers and cardiovascular and neurodegenerative diseases. For a long time, different <em>Ferulago</em> species have been in use as medicinal plants worldwide.<br> &nbsp;<strong>Objective: </strong>The aim of this research was to investigate total phenolics and the antioxidant and antibacterial activities of essential oil and different extracts from <em>F. angulata</em> growing wild in Iran.<br> &nbsp;<strong>Methods: </strong>Antioxidant activity of our samples was examined by DPPH assay and their phenolic content was determined using the Folin &ndash; Ciocalteau method. Moreover, their inhibitory effects against five gram-negative and gram-positive bacteria including <em>Shigella boidii</em>,<em> Pseudomonas aeruginosa</em>,<em> Escherichia coli</em>,<em> Staphylococcus aureus and Enterococcus faecalis</em> were tested by using the disk diffusion method.<br> &nbsp;<strong>Results:</strong>&nbsp;Ethyl acetate fraction and methanol extract contained the highest levels of phenolic compounds (229.2 and 202.9 &micro;g/mg&nbsp;galic acid) in this study. Also, essential oil of this plant exhibited low free radical scavenging activity (IC₅₀= 36129.43&micro;g/ml). On the other hand, ethyl acetate and aqueous fractions had the highest antioxidant activity (IC₅₀ values of 20.153 and 28.28 &micro;g/ml, respectively). Finally, studied samples had no significant antibacterial activities against tested organisms.<br> &nbsp;<strong>Conclusion:&nbsp;</strong>The essential oil of this plant can be used as a flavoring agent while extracts prepared from it have the potential to be used as natural antioxidants in relevant industries.</div> <div dir="ltr" style="text-align: justify;">&nbsp;<strong>Background:</strong>&nbsp;In recent years, plants natural products have gained considerable attention as natural preservers for use in different industries. Due to their free radical scavenging activities, phenolic compounds can prevent a wide range of diseases such as cancers and cardiovascular and neurodegenerative diseases. For a long time, different <em>Ferulago</em> species have been in use as medicinal plants worldwide.<br> &nbsp;<strong>Objective: </strong>The aim of this research was to investigate total phenolics and the antioxidant and antibacterial activities of essential oil and different extracts from <em>F. angulata</em> growing wild in Iran.<br> &nbsp;<strong>Methods: </strong>Antioxidant activity of our samples was examined by DPPH assay and their phenolic content was determined using the Folin &ndash; Ciocalteau method. Moreover, their inhibitory effects against five gram-negative and gram-positive bacteria including <em>Shigella boidii</em>,<em> Pseudomonas aeruginosa</em>,<em> Escherichia coli</em>,<em> Staphylococcus aureus and Enterococcus faecalis</em> were tested by using the disk diffusion method.<br> &nbsp;<strong>Results:</strong>&nbsp;Ethyl acetate fraction and methanol extract contained the highest levels of phenolic compounds (229.2 and 202.9 &micro;g/mg&nbsp;galic acid) in this study. Also, essential oil of this plant exhibited low free radical scavenging activity (IC₅₀= 36129.43&micro;g/ml). On the other hand, ethyl acetate and aqueous fractions had the highest antioxidant activity (IC₅₀ values of 20.153 and 28.28 &micro;g/ml, respectively). Finally, studied samples had no significant antibacterial activities against tested organisms.<br> &nbsp;<strong>Conclusion:&nbsp;</strong>The essential oil of this plant can be used as a flavoring agent while extracts prepared from it have the potential to be used as natural antioxidants in relevant industries.</div> ,Ferulago angulata ssp. angulata, Antioxidant activity, Antibacterial activity, Total phenolics Ferulago angulata ssp. angulata, Antioxidant activity, Antibacterial activity, Total phenolics 80 89 http://jmp.ir/browse.php?a_code=A-10-125-1&slc_lang=en&sid=1 N Hosseini N Hosseini na_hosseini@ymail.com 100319475328460042248 100319475328460042248 Yes Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran M Akbari M Akbari 100319475328460042249 100319475328460042249 No Department of Microbiology, Arak University of Medical Sciences, Arak, Iran Department of Microbiology, Arak University of Medical Sciences, Arak, Iran R Ghafarzadegan R Ghafarzadegan 100319475328460042250 100319475328460042250 No Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran S Changizi Ashtiyani S Changizi Ashtiyani 100319475328460042251 100319475328460042251 No Department of Physiology, Arak University of Medical Sciences, Arak, Iran Department of Physiology, Arak University of Medical Sciences, Arak, Iran R Shahmohammadi R Shahmohammadi 100319475328460042252 100319475328460042252 No Department of Biology, University of Guilan, Rasht, Iran Department of Biology, University of Guilan, Rasht, Iran