en Cytotoxicity and Antioxidant Activity of Five Plant Species of Solanaceae Family from Iran فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div dir="ltr" style="text-align: justify;">&nbsp;<strong>Background:</strong>&nbsp;The use of natural products as anticancer and antioxidant agents has a long history. Several drugs currently used in chemotherapy were isolated from plant species.<br> &nbsp;<strong>Objective:</strong>&nbsp;The aim of this study was to evaluate cytotoxicity and antioxidant activity as well as phenol and flavonoid contents of five plant species of Solanaceae family.<br> &nbsp;<strong>Methods:</strong>&nbsp;Five plant species of Solanaceae family were collected from different regions of Iran. Methanol extracts and chloroform fractions of these species were tested by brine shrimp lethality assay in order to detect cytotoxicity. Antioxidant activity was evaluated by DPPH method. The total phenol content was measured using Folin - Ciocalteu method. The flavonoid content was measured by a colorimetric assay.<br> &nbsp;<strong>Results:</strong>&nbsp;The extracts of <em>Datura innoxia</em> and <em>Datura stramonium</em> showed the highest cytotoxicity activities with LC₅₀ values of 22.08 and 21.66 &mu;g/ml, respectively. The chloroform fractions of these two species were subjected to cytotoxicity assay with LC₅₀ values of 33.00 and 4.29 &mu;g/ml, respectively. In comparing, <em>Solanum dulcamara</em> showed the highest antioxidant activity with IC₅₀ values of 52.51 &mu;g/ml and the highest phenol and flavonoid content of the dry weight.<br> &nbsp;<strong>Conclusion:&nbsp;</strong>It could be seen among five tested plant species that <em>D. stramonium</em> had the highest cytotoxic activity and <em>S. dulcamara</em> had the highest antioxidant activity, phenol and flavonoid content. Further studies are necessary for chemical composition of the extracts and more comprehensive biological assays.<br> &nbsp;</div> <div dir="ltr" style="text-align: justify;">&nbsp;<strong>Background:</strong>&nbsp;The use of natural products as anticancer and antioxidant agents has a long history. Several drugs currently used in chemotherapy were isolated from plant species.<br> &nbsp;<strong>Objective:</strong>&nbsp;The aim of this study was to evaluate cytotoxicity and antioxidant activity as well as phenol and flavonoid contents of five plant species of Solanaceae family.<br> &nbsp;<strong>Methods:</strong>&nbsp;Five plant species of Solanaceae family were collected from different regions of Iran. Methanol extracts and chloroform fractions of these species were tested by brine shrimp lethality assay in order to detect cytotoxicity. Antioxidant activity was evaluated by DPPH method. The total phenol content was measured using Folin - Ciocalteu method. The flavonoid content was measured by a colorimetric assay.<br> &nbsp;<strong>Results:</strong>&nbsp;The extracts of <em>Datura innoxia</em> and <em>Datura stramonium</em> showed the highest cytotoxicity activities with LC₅₀ values of 22.08 and 21.66 &mu;g/ml, respectively. The chloroform fractions of these two species were subjected to cytotoxicity assay with LC₅₀ values of 33.00 and 4.29 &mu;g/ml, respectively. In comparing, <em>Solanum dulcamara</em> showed the highest antioxidant activity with IC₅₀ values of 52.51 &mu;g/ml and the highest phenol and flavonoid content of the dry weight.<br> &nbsp;<strong>Conclusion:&nbsp;</strong>It could be seen among five tested plant species that <em>D. stramonium</em> had the highest cytotoxic activity and <em>S. dulcamara</em> had the highest antioxidant activity, phenol and flavonoid content. Further studies are necessary for chemical composition of the extracts and more comprehensive biological assays.<br> &nbsp;</div> Antioxidant, Cytotoxicity, Flavonoid, Phenol, Solanaceae Antioxidant, Cytotoxicity, Flavonoid, Phenol, Solanaceae 41 53 http://jmp.ir/browse.php?a_code=A-10-123-1&slc_lang=en&sid=1 F Khalighi-Sigaroodi F Khalighi-Sigaroodi khalighi@imp.ac.ir 100319475328460036915 100319475328460036915 Yes Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR M Ahvazi Ahvazi 100319475328460036916 100319475328460036916 No Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran D Yazdani D Yazdani 100319475328460036917 100319475328460036917 No Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran M Kashefi M Kashefi 100319475328460036918 100319475328460036918 No Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran