en Antibacterial Activity of <i>Origanum vulgare</i> on <i>Staphylococcus aureus</i> in a Rat Model of Surgical Wound Infection فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Wound infection is one of the frequent complications in patients with surgical operations. <em>Staphylococcus aureus</em> is the most common cause of surgical wound infections. <em>Origanum vulgare</em>, a common culinary herb, has been shown to have strong antimicrobial activity, especially against Gram-positive pathogens.<br> <strong>Objective:</strong> This study was designed to investigate the antibacterial effects of <em>O. vulgare</em> on <em>S. aureus</em> in surgical wounds using a rat model.<br> <strong>Methods:</strong> Twenty male Sprague-Dawley rats were divided randomly into two groups of treatment and control rats (1:1). A circular incision was made on the dorsal inter-scapular region of each rat. Then, rats were inoculated topically with 1 &times; 10⁴ CFU of <em>S. aureus </em>at the site of skin wounds. <em>O. vulgare</em> extract was applied to wounds twice a day during the experiment. Animals of the control group were left untreated.<br> <strong>Results:</strong> The load of bacteria in untreated rats was 7 &times; 10⁶&plusmn; 6 CFU/wound while this was 2 &times; 10⁵ &plusmn;1 CFU/wound for the treatment animals, which was significantly lower.<br> <strong>Conclusion:</strong> Results have showed that topical application of <em>O. vulgare</em> extract on the infected wounds included significant antibacterial activity against <em>S. aureus</em>.</div> <div style="text-align: justify;"><strong>Background:</strong> Wound infection is one of the frequent complications in patients with surgical operations. <em>Staphylococcus aureus</em> is the most common cause of surgical wound infections. <em>Origanum vulgare</em>, a common culinary herb, has been shown to have strong antimicrobial activity, especially against Gram-positive pathogens.<br> <strong>Objective:</strong> This study was designed to investigate the antibacterial effects of <em>O. vulgare</em> on <em>S. aureus</em> in surgical wounds using a rat model.<br> <strong>Methods:</strong> Twenty male Sprague-Dawley rats were divided randomly into two groups of treatment and control rats (1:1). A circular incision was made on the dorsal inter-scapular region of each rat. Then, rats were inoculated topically with 1 &times; 10⁴ CFU of <em>S. aureus </em>at the site of skin wounds. <em>O. vulgare</em> extract was applied to wounds twice a day during the experiment. Animals of the control group were left untreated.<br> <strong>Results:</strong> The load of bacteria in untreated rats was 7 &times; 10⁶&plusmn; 6 CFU/wound while this was 2 &times; 10⁵ &plusmn;1 CFU/wound for the treatment animals, which was significantly lower.<br> <strong>Conclusion:</strong> Results have showed that topical application of <em>O. vulgare</em> extract on the infected wounds included significant antibacterial activity against <em>S. aureus</em>.</div> ,Origanum vulgare, Staphylococcus aureus, Surgical wound Origanum vulgare, Staphylococcus aureus, Surgical wound 19 24 http://jmp.ir/browse.php?a_code=A-10-1165-1&slc_lang=en&sid=1 M Rahmani Gohar M Rahmani Gohar 100319475328460041584 100319475328460041584 No Graduated from Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran Graduated from Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran HR Moslemi HR Moslemi 100319475328460041585 100319475328460041585 No Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran KH Kafshdouzan KH Kafshdouzan kafshdouzan@semnan.ac.ir 100319475328460041586 100319475328460041586 Yes Department of Microbiology, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran Department of Microbiology, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran R Mazaheri Nezhad Fard R Mazaheri Nezhad Fard 100319475328460041587 100319475328460041587 No Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran