Journal of Medicinal Plants

en The potential co-treatment effects of Zataria multiflora, Camellia sinensis extracts and bacterial Lipopolysaccharide on skin wound model The potential co-treatment effects of Zataria multiflora, Camellia sinensis extracts and bacterial Lipopolysaccharide on skin wound model فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research Background: Camellia sinensis and Zataria multiflora as the potent antioxidants and anti-inflammations are crucial in the management of wound therapy. Lipopolysaccharide (LPS) was used for the proliferative potential on fibroblast cells and induction of inflammatory mediators. Objective: We examined the effects of LPS and herbal extracts combination in order to identify their mechanisms of action in fibroblast proliferation and tissue regeneration. Methods: Human foreskin fibroblasts were treated by Salmonella enterica LPS (100μg) and extracts (5%w/w). Tissues of male Balb/c mice were harvested at 1, 3 and 7 days for biochemical and histopathological evaluations. Effects of LPS and extracts on cell viability, Nitric oxide (NO), Cyclooxygenase-2 (COX-2) and Hydrogen peroxide (H2O2) levels were examined respectively by XTT assay and related kits. Results: Our pathological investigations for Camellia sinensis and LPS co-treated group indicated inflammatory cells on day 1 and fibroblast proliferation through wound area on day 3. After wound modelling the samples features were the same but with the difference in groups treated by LPS and Camellia sinensis extract which dermis and epidermis were seen. The Camellia sinensis extract and LPS co-treated wounds were showed low levels of H2O2 and higher levels of NO compared to extract treated group (P<0.001). Results illustrate a dose and time dependent significant difference in cell proliferation between groups. Conclusion: These results suggest that Camellia sinensis extract in combination with LPS may have potential of accelerating inflammatory phase of wound healing process by regulation of COX-2, NO and H2O2 in skin fibroblast. Background: Camellia sinensis and Zataria multiflora as the potent antioxidants and anti-inflammations are crucial in the management of wound therapy. Lipopolysaccharide (LPS) was used for the proliferative potential on fibroblast cells and induction of inflammatory mediators. Objective: We examined the effects of LPS and herbal extracts combination in order to identify their mechanisms of action in fibroblast proliferation and tissue regeneration. Methods: Human foreskin fibroblasts were treated by Salmonella enterica LPS (100μg) and extracts (5%w/w). Tissues of male Balb/c mice were harvested at 1, 3 and 7 days for biochemical and histopathological evaluations. Effects of LPS and extracts on cell viability, Nitric oxide (NO), Cyclooxygenase-2 (COX-2) and Hydrogen peroxide (H2O2) levels were examined respectively by XTT assay and related kits. Results: Our pathological investigations for Camellia sinensis and LPS co-treated group indicated inflammatory cells on day 1 and fibroblast proliferation through wound area on day 3. After wound modelling the samples features were the same but with the difference in groups treated by LPS and Camellia sinensis extract which dermis and epidermis were seen. The Camellia sinensis extract and LPS co-treated wounds were showed low levels of H2O2 and higher levels of NO compared to extract treated group (P<0.001). Results illustrate a dose and time dependent significant difference in cell proliferation between groups. Conclusion: These results suggest that Camellia sinensis extract in combination with LPS may have potential of accelerating inflammatory phase of wound healing process by regulation of COX-2, NO and H2O2 in skin fibroblast. ,Camellia sinensis,Zataria multiflora, LPS, Wound model Camellia sinensis, Zataria multiflora, LPS, Wound model 56 73 http://jmp.ir/browse.php?a_code=A-10-958-1&slc_lang=en&sid=1 HR Ahmadi Ashtiani حمیدرضا احمدی آشتیانی ahmadihr@yahoo.com 100319475328460041606 100319475328460041606 Yes Academic member عضو هیات علمی H Rastegar حسین رستگار mhrastegar2@yahoo.com 100319475328460041607 100319475328460041607 No Academic member عضو هیات علمی SM Rezayat سید مهدی رضایت rezayat@tums.ac.ir 100319475328460041608 100319475328460041608 No Academic member عضو هیات علمی Sh Rezazadeh شمسعلی رضازاده shrezazadeh@yahoo.com 100319475328460041609 100319475328460041609 No Institute of Medicinal Plants (ACECR), Karaj, Iran Institute of Medicinal Plants (ACECR), Karaj, Iran O Ghorban Dadras قربان دادرس 100319475328460041610 100319475328460041610 No Islamic Azad University, Pharmaceutical Sciences Branch, Tehran, Iran دانشگاه آزاد اسلامی، واحد علوم دارویی S Anjarani آنجرانی 100319475328460041611 100319475328460041611 No Health Reference Laboratory, Iranian FDA, MOH & Medical Education, Tehran, Iran آزمایشگاه مرجع سلامت ، وزارت بهداشت و آموزش پزشکی ، تهران ، ایران Sh Heydarzadeh حیدرزاده 100319475328460041612 100319475328460041612 No Biology Department of Cepigène Co., Tehran, Iran گروه زیست شناسی MM Naderi نادری 100319475328460041613 100319475328460041613 No Reproductive Biotechnology Research Center, Avicenna Research Institute, Tehran, Iran مرکز تحقیقات بیوتکنولوژی باروری ، پژوهشکده ابن سینا ، تهران ، ایران