en Evaluation of antibacterial activity and flavonoid content of two <i>Capparis</i> species from Iran فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <p dir="ltr" style="text-align: justify; margin-left: 14.05pt;"><strong>Background:</strong> Due to multiple drug resistance against antimicrobial drugs for treatment of infectious disease, investigations to discovering new antibacterial compounds from natural sources have been designated.</p> <p dir="ltr" style="text-align: justify; margin-left: 14.05pt;"><strong>Objective:</strong> According to antimicrobial effects of some Capparis species, this study was carried out to evaluate the antimicrobial activity of the extract and fractions of <em>Capparis cartilaginea</em> and <em>Capparis mucronifolia</em> against 6 bacterial strains.</p> <p dir="ltr" style="text-align: justify; margin-left: 14.05pt;"><strong>Methods:</strong> Aerial parts of the Capparis species were extracted by maceration method using methanol and fractionated separately by liquid-liquid fractionation method. The antibacterial activity of the extract and fractions were studied against 6 bacterial strains using MIC microplate method. Total flavonoid content (TFC) of the extracts and fractions was determined using AlCl₃ reagent.</p> <p dir="ltr" style="text-align: justify; margin-left: 14.05pt;"><strong>Results:</strong> The total extract and methanolic fractions of <em>C. mucronifolia</em> were the most effective fractions against the bacterial strains. Methanolic fraction of <em>C. cartilaginea</em> was the most effective fraction with MIC 10.42 &micro;g/ml against <em>Salmonella enterica.</em> The highest antibacterial activity of <em>C.mucronifolia</em> was against <em>Staphylococcus epidermidis</em> with MIC 7.8 &micro;g/ml. Methanolic fractions of Capparis species showed the highest TFC value in AlCl₃ colorimetric assay.</p> <p dir="ltr" style="text-align: justify; margin-left: 14.05pt;"><strong>Conclusion:</strong> The results of this study indicate that the extracts and fractions of<em> Capparis cartilaginea </em>and<em> Capparis mucronifolia</em> have antimicrobial effect against 6 gram positive and gram negative strains. According to the high amount of flavonoids in methanolic fraction of<br> <em>C. mucronifolia</em>, the antibacterial activity may be related to the flavonoid compounds of this plant.</p> <div dir="ltr"></div> <p style="margin-right: 14.05pt; text-align: justify;"><strong>Background:</strong> Due to multiple drug resistance against antimicrobial drugs for treatment of infectious disease, investigations to discovering new antibacterial compounds from natural sources have been designated.</p> <p style="margin-right: 14.05pt; text-align: justify;"><strong>Objective:</strong> According to antimicrobial effects of some Capparis species, this study was carried out to evaluate the antimicrobial activity of the extract and fractions of <em>Capparis cartilaginea</em> and <em>Capparis mucronifolia</em> against 6 bacterial strains.</p> <p style="margin-right: 14.05pt; text-align: justify;"><strong>Methods:</strong> Aerial parts of the Capparis species were extracted by maceration method using methanol and fractionated separately by liquid-liquid fractionation method. The antibacterial activity of the extract and fractions were studied against 6 bacterial strains using MIC microplate method. Total flavonoid content (TFC) of the extracts and fractions was determined using AlCl₃ reagent.</p> <p style="margin-right: 14.05pt; text-align: justify;"><strong>Results:</strong> The total extract and methanolic fractions of <em>C. mucronifolia</em> were the most effective fractions against the bacterial strains. Methanolic fraction of <em>C. cartilaginea</em> was the most effective fraction with MIC 10.42 &micro;g/ml against <em>Salmonella enterica.</em> The highest antibacterial activity of <em>C.mucronifolia</em> was against <em>Staphylococcus epidermidis</em> with MIC 7.8 &micro;g/ml. Methanolic fractions of Capparis species showed the highest TFC value in AlCl₃ colorimetric assay.</p> <p style="margin-right: 14.05pt; text-align: justify;"><strong>Conclusion:</strong> The results of this study indicate that the extracts and fractions of<em> Capparis cartilaginea </em>and<em> Capparis mucronifolia</em> have antimicrobial effect against 6 gram positive and gram negative strains. According to the high amount of flavonoids in methanolic fraction of<br> <em>C. mucronifolia</em>, the antibacterial activity may be related to the flavonoid compounds of this plant.</p> <p style="margin-right:14.05pt"></p><p></p> ,Capparis cartilaginea, Capparis mucronifolia, Antimicrobial activity, Flavonoid, MIC Capparis cartilaginea, Capparis mucronifolia, Antimicrobial activity, Flavonoid, MIC 89 94 http://jmp.ir/browse.php?a_code=A-10-715-2&slc_lang=en&sid=1 N Rahimifard N Rahimifard 100319475328460044157 100319475328460044157 No Food and Drug Laboratory Research center (FDLRC), Food and Drug Control Laboratories (FDCLs), Ministry of Health (MOH), Pharmaceutical sciences branch, Islamic Azad University, Tehran, Iran دانشگاه آزاد اسلامی A Shojaii A Shojaii Shojaii.a@iums.ac.ir 100319475328460044158 100319475328460044158 Yes Research Institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی ایران M Mahbobi M Mahbobi 100319475328460044159 100319475328460044159 No Pharmacognosy Department, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran دانشگاه آزاد اسلامی Gh Hafezan Gh Hafezan 100319475328460044160 100319475328460044160 No Pharmacognosy Department, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran دانشگاه آزاد اسلامی F Bagheri F Bagheri 100319475328460044161 100319475328460044161 No Microbiology Department, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran دانشگاه آزاد اسلامی J Asgarpanah J Asgarpanah 100319475328460044162 100319475328460044162 No Pharmacognosy Department, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran دانشگاه آزاد اسلامی