Journal of Medicinal Plants

en Encapsulation of Green Tea Extract in Nanoliposomes and Evaluation of its Antibacterial, Antioxidant and Prebiotic Properties Encapsulation of Green Tea Extract in Nanoliposomes and Evaluation of its Antibacterial, Antioxidant and Prebiotic Properties فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research Background: The application of natural compounds including green tea extract (GTE) in food and pharmaceutical industries is limited. Encapsulation in nanoliposomes could be used as a delivery system to protect these compounds during processing and storage. Objective: In this study encapsulation of green tea extract in nanoliposomes and evaluation of its antibacterial, antioxidant and prebiotic properties were evaluated. Methods: GTE was encapsulated in liposomes by thin film layer method and reached to nanoscale with sonication. The prebiotic activity of 1% nanoliposomal GTE was evaluated on the growth of lactobacillus casei and bifidobacterium lactis in MRS broth medium. Furthermore, the antioxidant activity of nanoliposomal GTE was estimated by DPPH assay. The antibacterial activity of nanoliposomal GTE against Bacillus cereus (ATCC11778), Salmonella typhimurium 138 phage type 2, E. coli O157:H7 and Listeria monocytogenes (ATCC19118) was determined using well diffusion technique. Results: The mean diameter of nanoliposomes was about 44.7± 1.9 nm and had 0.203 ±0.014 polydispersity index. Entrapment eﬃciency of nanoliposomal GTE under the optimum conditions was 97%. Antibacterial activity of GTE was signiﬁcantly increased after encapsulation in nanoliposomes. The strongest antibacterial activity of nanoliposomal GTE was seen against listeria monocytogenes with an inhibition zone of 16.2 mm while E. coli was the most resistance strain with an inhibition zone of 14 mm. Furthermore, the antioxidant activity of GTE was signiﬁcantly increased after nanoliposome encapsulation since the IC50 value of nanoliposomal GTE was decreased to 1.78 &mug ml-1. Moreover, addition of 1 % nanoliposomal GTE enhanced the growth rate of Lactobacillus casei and Bifidobacterium lactis to a signiﬁcant extent. Conclusion: Nanoencapsulation effectively enhanced beneficial properties of GTE. <div dir="ltr"></div> Background: The application of natural compounds including green tea extract (GTE) in food and pharmaceutical industries is limited. Encapsulation in nanoliposomes could be used as a delivery system to protect these compounds during processing and storage. Objective: In this study encapsulation of green tea extract in nanoliposomes and evaluation of its antibacterial, antioxidant and prebiotic properties were evaluated. Methods: GTE was encapsulated in liposomes by thin film layer method and reached to nanoscale with sonication. The prebiotic activity of 1% nanoliposomal GTE was evaluated on the growth of lactobacillus casei and bifidobacterium lactis in MRS broth medium. Furthermore, the antioxidant activity of nanoliposomal GTE was estimated by DPPH assay. The antibacterial activity of nanoliposomal GTE against Bacillus cereus (ATCC11778), Salmonella typhimurium 138 phage type 2, E. coli O157:H7 and Listeria monocytogenes (ATCC19118) was determined using well diffusion technique. Results: The mean diameter of nanoliposomes was about 44.7± 1.9 nm and had 0.203 ±0.014 polydispersity index. Entrapment eﬃciency of nanoliposomal GTE under the optimum conditions was 97%. Antibacterial activity of GTE was signiﬁcantly increased after encapsulation in nanoliposomes. The strongest antibacterial activity of nanoliposomal GTE was seen against listeria monocytogenes with an inhibition zone of 16.2 mm while E. coli was the most resistance strain with an inhibition zone of 14 mm. Furthermore, the antioxidant activity of GTE was signiﬁcantly increased after nanoliposome encapsulation since the IC50 value of nanoliposomal GTE was decreased to 1.78 &mug ml-1. Moreover, addition of 1 % nanoliposomal GTE enhanced the growth rate of Lactobacillus casei and Bifidobacterium lactis to a signiﬁcant extent. Conclusion: Nanoencapsulation effectively enhanced beneficial properties of GTE. Antibacterial activity, Antioxidant activity, Green tea extract, Nanoliposome encapsulation, Prebiotic properties Antibacterial activity, Antioxidant activity, Green tea extract, Nanoliposome encapsulation, Prebiotic properties 66 78 http://jmp.ir/browse.php?a_code=A-10-766-3&slc_lang=en&sid=1 B Noudoost B Noudoost 100319475328460036108 100319475328460036108 No Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran دانشگاه تهران N Noori N Noori 100319475328460036109 100319475328460036109 Yes Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran دانشگاه تهران Gh Amo Abedini Gh Amo Abedini 100319475328460036110 100319475328460036110 No Department of Chemical Engineering, School of Engineering, University of Tehran, Tehran, Iran دانشگاه تهران H Gandomi H Gandomi 100319475328460036111 100319475328460036111 No Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran دانشگاه تهران A Akhondzadeh Basti A Akhondzadeh Basti 100319475328460036112 100319475328460036112 No Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran دانشگاه تهران A Jebeli Javan A Jebeli Javan 100319475328460036113 100319475328460036113 No Department of Food Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran دانشگاه سمنان F Ghadami F Ghadami 100319475328460036114 100319475328460036114 No Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran دانشگاه تهران