en Phytochemical and Wound Healing Effects of Methanolic Extract of <i>Salvia multicaulis</i> Vahl. in Rat فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Background:</strong> Plants and their phytochemicals have immense potential for the management of different types of wound.<em> Salvia multicaulis </em>Vahl. is an evergreen shrub species, which traditionally used as a wound healing agent.</p> <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Objective: </strong>The aim of the present study is to evaluate phytochemicals and wound healing activity of <em>S.</em> <em>multicaulis</em> in an excision wound model.</p> <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Methods:</strong> Preliminary phytochemical screening tests were performed on the methanol (80%) extract. Total phenolic content was measured using Folin-Ciocalteu reagent. Antioxidant activity of the extract was assessed by (DPPH) free radical scavenging test. 2x2 excision wounds were induced in the dorsal area of 30 Wistar rats. Animals were received topical eucerine, 1% phenytoin as well as 5%, 10% and 20% ointments of the plant extract in eucerine base. Wound areas were photographed and wound contraction was calculated as the reduction percentage of wound size compared with zero days. Last day skin samples of wounded area were used for histological evaluations.</p> <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Results:</strong> Collagen deposition for positive control group and A20% were low fibrils and A10% high fibrils and A5% highest fibrils. Total phenolic content was 262.083 &plusmn; 47.15 mg GAE/g extract. IC50 was 54.02 &micro;g/mL. All concentrations of the extract ointments were significantly effective in the animal model of excision wound (P<0.05) which was also confirmed in histological study.</p> <p dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> It is concluded that <em>S.</em> <em>multicaulis</em> extract could act as a wound healing agent. This activity is possibly due the high concentrations of phenolic compounds.</p> <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Background:</strong> Plants and their phytochemicals have immense potential for the management of different types of wound.<em> Salvia multicaulis </em>Vahl. is an evergreen shrub species, which traditionally used as a wound healing agent.</p> <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Objective: </strong>The aim of the present study is to evaluate phytochemicals and wound healing activity of <em>S.</em> <em>multicaulis</em> in an excision wound model.</p> <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Methods:</strong> Preliminary phytochemical screening tests were performed on the methanol (80%) extract. Total phenolic content was measured using Folin-Ciocalteu reagent. Antioxidant activity of the extract was assessed by (DPPH) free radical scavenging test. 2x2 excision wounds were induced in the dorsal area of 30 Wistar rats. Animals were received topical eucerine, 1% phenytoin as well as 5%, 10% and 20% ointments of the plant extract in eucerine base. Wound areas were photographed and wound contraction was calculated as the reduction percentage of wound size compared with zero days. Last day skin samples of wounded area were used for histological evaluations.</p> <p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Results:</strong> Collagen deposition for positive control group and A20% were low fibrils and A10% high fibrils and A5% highest fibrils. Total phenolic content was 262.083 &plusmn; 47.15 mg GAE/g extract. IC50 was 54.02 &micro;g/mL. All concentrations of the extract ointments were significantly effective in the animal model of excision wound (P<0.05) which was also confirmed in histological study.</p> <p dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> It is concluded that <em>S.</em> <em>multicaulis</em> extract could act as a wound healing agent. This activity is possibly due the high concentrations of phenolic compounds.</p> Wound, Salvia multicaulis, Vahl, Antioxidant, Total phenol, Exision Wound, Salvia multicaulis, Vahl, Antioxidant, Total phenol, Exision 38 46 http://jmp.ir/browse.php?a_code=A-10-874-1&slc_lang=en&sid=1 I Salimikia I Salimikia 100319475328460041594 100319475328460041594 No Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran M Aryanpour M Aryanpour 100319475328460041595 100319475328460041595 No Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran R Bahramsoltani R Bahramsoltani 100319475328460041596 100319475328460041596 No Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran M Abdollahi M Abdollahi 100319475328460041597 100319475328460041597 No Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran AH Abdolghaffari AH Abdolghaffari 100319475328460041598 100319475328460041598 No Pharmacology and Applied Medicine, Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Pharmacology and Applied Medicine, Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran N Samadi N Samadi 100319475328460041599 100319475328460041599 No Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran F Sabbaghziarani F Sabbaghziarani 100319475328460041600 100319475328460041600 No Department of Anatomy, School of Medicine, Tehran Medical University, Tehran, Iran Department of Anatomy, School of Medicine, Tehran Medical University, Tehran, Iran M Gholami M Gholami 100319475328460041601 100319475328460041601 No Department of Toxicology and Pharmacology, Faculty of Pharmacy; and Pharmaceutical Sciences Research Center; and Poisoning & Toxicology Research Center; and Endocrinology & Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute Tehran University of Medical Sciences, Tehran, Iran Department of Toxicology and Pharmacology, Faculty of Pharmacy; and Pharmaceutical Sciences Research Center; and Poisoning & Toxicology Research Center; and Endocrinology & Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute Tehran University of Medical Sciences, Tehran, Iran HR HR Monsef-Esfahani iraj_t33@yahoo.com 100319475328460041602 100319475328460041602 Yes Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences /University of Tehran, Tehran, Iran Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences /University of Tehran, Tehran, Iran