Journal of Medicinal Plants
فصلنامه گياهان دارویی
J. Med. Plants
Medical Sciences
http://jmp.ir
1
admin
2717-204X
2717-2058
10.61186/jmp
14
8888
13
en
jalali
1394
11
1
gregorian
2016
2
1
15
57
online
1
fulltext
en
Phytochemical and Wound Healing Effects of Methanolic Extract of <i>Salvia multicaulis</i> Vahl. in Rat
Phytochemical and Wound Healing Effects of Methanolic Extract of <i>Salvia multicaulis</i> Vahl. in Rat
فارماكولوژی و سم شناسی
Pharmacology & Toxicology
پژوهشی
Research
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Background:</strong> Plants and their phytochemicals have immense potential for the management of different types of wound.<em> Salvia multicaulis </em>Vahl. is an evergreen shrub species, which traditionally used as a wound healing agent.</p>
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Objective: </strong>The aim of the present study is to evaluate phytochemicals and wound healing activity of <em>S.</em> <em>multicaulis</em> in an excision wound model.</p>
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Methods:</strong> Preliminary phytochemical screening tests were performed on the methanol (80%) extract. Total phenolic content was measured using Folin-Ciocalteu reagent. Antioxidant activity of the extract was assessed by (DPPH) free radical scavenging test. 2x2 excision wounds were induced in the dorsal area of 30 Wistar rats. Animals were received topical eucerine, 1% phenytoin as well as 5%, 10% and 20% ointments of the plant extract in eucerine base. Wound areas were photographed and wound contraction was calculated as the reduction percentage of wound size compared with zero days. Last day skin samples of wounded area were used for histological evaluations.</p>
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Results:</strong> Collagen deposition for positive control group and A20% were low fibrils and A10% high fibrils and A5% highest fibrils. Total phenolic content was 262.083 ± 47.15 mg GAE/g extract. IC50 was 54.02 µg/mL. All concentrations of the extract ointments were significantly effective in the animal model of excision wound (P<0.05) which was also confirmed in histological study.</p>
<p dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> It is concluded that <em>S.</em> <em>multicaulis</em> extract could act as a wound healing agent. This activity is possibly due the high concentrations of phenolic compounds.</p>
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Background:</strong> Plants and their phytochemicals have immense potential for the management of different types of wound.<em> Salvia multicaulis </em>Vahl. is an evergreen shrub species, which traditionally used as a wound healing agent.</p>
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Objective: </strong>The aim of the present study is to evaluate phytochemicals and wound healing activity of <em>S.</em> <em>multicaulis</em> in an excision wound model.</p>
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Methods:</strong> Preliminary phytochemical screening tests were performed on the methanol (80%) extract. Total phenolic content was measured using Folin-Ciocalteu reagent. Antioxidant activity of the extract was assessed by (DPPH) free radical scavenging test. 2x2 excision wounds were induced in the dorsal area of 30 Wistar rats. Animals were received topical eucerine, 1% phenytoin as well as 5%, 10% and 20% ointments of the plant extract in eucerine base. Wound areas were photographed and wound contraction was calculated as the reduction percentage of wound size compared with zero days. Last day skin samples of wounded area were used for histological evaluations.</p>
<p dir="ltr" style="margin-left: 13.9pt; text-align: justify;"><strong>Results:</strong> Collagen deposition for positive control group and A20% were low fibrils and A10% high fibrils and A5% highest fibrils. Total phenolic content was 262.083 ± 47.15 mg GAE/g extract. IC50 was 54.02 µg/mL. All concentrations of the extract ointments were significantly effective in the animal model of excision wound (P<0.05) which was also confirmed in histological study.</p>
<p dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> It is concluded that <em>S.</em> <em>multicaulis</em> extract could act as a wound healing agent. This activity is possibly due the high concentrations of phenolic compounds.</p>
Wound, Salvia multicaulis, Vahl, Antioxidant, Total phenol, Exision
Wound, Salvia multicaulis, Vahl, Antioxidant, Total phenol, Exision
38
46
http://jmp.ir/browse.php?a_code=A-10-874-1&slc_lang=en&sid=1
I
Salimikia
I
Salimikia
100319475328460041594
100319475328460041594
No
Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
M
Aryanpour
M
Aryanpour
100319475328460041595
100319475328460041595
No
Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran
R
Bahramsoltani
R
Bahramsoltani
100319475328460041596
100319475328460041596
No
Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran
M
Abdollahi
M
Abdollahi
100319475328460041597
100319475328460041597
No
Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran
Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran
AH
Abdolghaffari
AH
Abdolghaffari
100319475328460041598
100319475328460041598
No
Pharmacology and Applied Medicine, Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Pharmacology and Applied Medicine, Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
N
Samadi
N
Samadi
100319475328460041599
100319475328460041599
No
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
F
Sabbaghziarani
F
Sabbaghziarani
100319475328460041600
100319475328460041600
No
Department of Anatomy, School of Medicine, Tehran Medical University, Tehran, Iran
Department of Anatomy, School of Medicine, Tehran Medical University, Tehran, Iran
M
Gholami
M
Gholami
100319475328460041601
100319475328460041601
No
Department of Toxicology and Pharmacology, Faculty of Pharmacy; and Pharmaceutical Sciences Research Center; and Poisoning & Toxicology Research Center; and Endocrinology & Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute Tehran University of Medical Sciences, Tehran, Iran
Department of Toxicology and Pharmacology, Faculty of Pharmacy; and Pharmaceutical Sciences Research Center; and Poisoning & Toxicology Research Center; and Endocrinology & Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute Tehran University of Medical Sciences, Tehran, Iran
HR
HR
Monsef-Esfahani
iraj_t33@yahoo.com
100319475328460041602
100319475328460041602
Yes
Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences /University of Tehran, Tehran, Iran
Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences /University of Tehran, Tehran, Iran