en Phytochemical diversity of <i>Eremostachys laciniata</i> Bunge populations in Iran پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background</strong>: <em>Eremostachys laciniata</em> is known as &quot;Chaelle-Daghi&quot; in Iran and its rhizomes are used as an emollient to relieve rheumatoid arthritis.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective</strong>: A phytochemical study was performed on 15 populations of<em> Eremostachys laciniata</em> to qualify the phytochemical variations.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods</strong>: Plants collected from their natural habitats and dried rhizomes of <em>E. laciniata</em> were milled and then Soxhlet-extracted by MeOH, and then total phenols were determined calorimetrically by the Folin-ciocalteau reagent and iridoid glycosides were measured by TLC method.</p> <p dir="ltr" style="text-align: justify;"><strong>Results</strong>: The results of MeOH extracts showed that the highest amount with 14.85 gr/plant was obtained in the Areshtanab population. The populations had a significant effect on total phenolic concentrations (p < 0.001) and the Areshtanab with 0.281 mg GAE/g DW had higher phenolic levels than other populations. The results of TLC of iridoids showed that 15 populations had about 11 iridoids components. Cluster analysis by using Ward&#39;s method detected that the studied populations of <em>E. laciniata</em> were separated into three different groups.</p> <p dir="ltr" style="text-align: justify;"><strong>Conclusion: </strong>In summary, higher values of the total phenols and iridoids in the Areshtanab, Malek-Kian, and Til populations were considered to indicate higher levels of phytochemical heterogeneity and significant diversity between populations, which could be used to domesticate and develop the breeding programs.</p> <p><strong>Background</strong>: <em>Eremostachys laciniata</em> is known as &quot;Chelle-Daghi&quot; in Iran and its rhizomes are used as an emollient to relieve rheumatoid arthritis.</p> <p><strong>Objective</strong>: A phytochemical study was performed on 15 populations of<em> Eremostachys laciniata</em> to qualify the phytochemical variations.</p> <p><strong>Methods</strong>: Plants collected from their natural habitats and dried rhizomes of <em>E. laciniata</em> were milled and then Soxhlet-extracted by MeOH, and then total phenols were determined calorimetrically by the Folin-ciocalteau reagent and iridoid glycosides were measured by TLC method.</p> <p><strong>Results</strong>: The results of MeOH extracts showed that the highest amount with 14.85 gr/plant was obtained in the Areshtanab population. The populations had a significant effect on total phenolic concentrations (p < 0.001) and the Areshtanab with 0.281 mg GAE/g DW had higher phenolic levels than other populations. The results of TLC of iridoids showed that 15 populations had about 11 iridoids components. Cluster analysis by using Ward&#39;s method detected that the studied populations of <em>E. laciniata</em> were separated into three different groups.</p> <p><strong>Conclusion: </strong>In summary, higher values of the total phenols and iridoids in the Areshtanab, Malek-Kian, and Til populations were considered to indicate higher levels of phytochemical heterogeneity and significant diversity between populations, which could be used to domesticate and develop the breeding programs.</p> ,Eremostachys laciniata, Bunge, Phytochemical diversity, Iridoids, Total phenol Eremostachys laciniata, Bunge, Phytochemical diversity, Iridoids, Total phenol 9 18 http://jmp.ir/browse.php?a_code=A-10-44-95&slc_lang=en&sid=1 A Hadipour A Hadipour Hadipour.ali@gmail.com 100319475328460041578 100319475328460041578 Yes International Campus, Ferdowsi University of Mashhad, Mashhad, Iran International Campus, Ferdowsi University of Mashhad, Mashhad, Iran M Azizi M Azizi 100319475328460041579 100319475328460041579 No Department of Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Department of Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran H Naghdi-Badi H Naghdi-Badi naghdibadi@yahoo.com 100319475328460041580 100319475328460041580 No Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran J Panahandeh J Panahandeh 100319475328460041581 100319475328460041581 No Department of Horticulture, College of Agriculture, Tabriz University, Tabriz, Iran Department of Horticulture, College of Agriculture, Tabriz University, Tabriz, Iran A Delazar A Delazar 100319475328460041582 100319475328460041582 No Department of Pharmacognozy, School of Pharmacy, Tabriz University of Medicinal Science, Tabriz, Iran Department of Pharmacognozy, School of Pharmacy, Tabriz University of Medicinal Science, Tabriz, Iran H Aroei H Aroei 100319475328460041583 100319475328460041583 No Department of Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Department of Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran