en Comparison in chemical compositions of the essential oil from leaves of <i>Lippia citriodora</i> H.B.K. plantlets produced from micro-propagation and stem cutting بيوتكنولوژی Biotechnology پژوهشی Research <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Background:</strong> Lemon verbena (<em>Lippia citriodora</em> H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in the North region of Iran.</p> <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Objective:</strong> This comparative study was carried out on essential oil content and composition of <em>Lippia</em> <em>citriodora</em> H.B.K. from micro-propagated and rooted apical stem cutting plantlets. Methods: The micro-propagated plantlets were obtained by use of synthetic medium condition containing basal MS medium supplemented with 1 mg.l^-1 BAP, 0.5 mg.l^-1 IBA, 1 g.l^-1 activated charcoal, 30 g.l^-1 sucrose, and 7 g.l^-1 agars. Pre-acclimated plantlets were transferred to the research greenhouse. To determine the essential oil content and its components in two types of plantlets, leaves of <em>L. citriodora</em> were collected in August 2013 before flowering stage. The essential oils were extracted by Clevenger apparatus and their constituents were determined by GC-MS.</p> <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Results:</strong> The results indicated that thirty-two components were determined in the stem cutting derived plantlets. The main components of stem cutting plantlets were geranial (13.81%), limonene (11.41%), spathulenol (11.22%), and neral (11.21%). Thirty-five components were also obtained in the micro-propagated plantlets. The main components of micro-propagated plantlets were consisted of geranial (17.26%), neral (14.48%), 6-methyl-5-hepten-2-one (10.75%), and limonene (8.93%).</p> <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Conclusion:</strong> In general, the essential oil content of stem cutting plantlets was higher than micro-propagated plantlets, while the number of compounds and amount of geranial and neral in stem cutting plantlets was lower than micro-propagated plantlets.</p> <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Background:</strong> Lemon verbena (<em>Lippia citriodora</em> H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in the North region of Iran.</p> <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Objective:</strong> This comparative study was carried out on essential oil content and composition of <em>Lippia</em> <em>citriodora</em> H.B.K. from micro-propagated and rooted apical stem cutting plantlets. Methods: The micro-propagated plantlets were obtained by use of synthetic medium condition containing basal MS medium supplemented with 1 mg.l^-1 BAP, 0.5 mg.l^-1 IBA, 1 g.l^-1 activated charcoal, 30 g.l^-1 sucrose, and 7 g.l^-1 agars. Pre-acclimated plantlets were transferred to the research greenhouse. To determine the essential oil content and its components in two types of plantlets, leaves of <em>L. citriodora</em> were collected in August 2013 before flowering stage. The essential oils were extracted by Clevenger apparatus and their constituents were determined by GC-MS.</p> <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Results:</strong> The results indicated that thirty-two components were determined in the stem cutting derived plantlets. The main components of stem cutting plantlets were geranial (13.81%), limonene (11.41%), spathulenol (11.22%), and neral (11.21%). Thirty-five components were also obtained in the micro-propagated plantlets. The main components of micro-propagated plantlets were consisted of geranial (17.26%), neral (14.48%), 6-methyl-5-hepten-2-one (10.75%), and limonene (8.93%).</p> <p dir="ltr" style="margin-left: 14.05pt; text-align: justify;"><strong>Conclusion:</strong> In general, the essential oil content of stem cutting plantlets was higher than micro-propagated plantlets, while the number of compounds and amount of geranial and neral in stem cutting plantlets was lower than micro-propagated plantlets.</p> ,Lippia citriodora H.B.K., Essential oil, Geranial, Micro-propagated plantlets, Neral Lippia citriodora H.B.K., Essential oil, Geranial, Micro-propagated plantlets, Neral 79 88 http://jmp.ir/browse.php?a_code=A-10-44-94&slc_lang=en&sid=1 M Moradi مرادی 100319475328460041686 100319475328460041686 No Department of Horticulture, Science and Research branch, Islamic Azad University, Karaj, Iran دانشگاه آزاد اسلامی A Mehrafarin علی مهرآفرین a.mehrafarin@gmail.com 100319475328460041687 100319475328460041687 No Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran پژوهشکده گیاهان دارویی A Qaderi اردشیر قادری ardeshir582003@yahoo.com 100319475328460041688 100319475328460041688 No Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran پژوهشکده گیاهان دارویی H Naghdi Badi حسنعلی نقدی بادی Naghdibadi@yahoo.com 100319475328460041689 100319475328460041689 Yes Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran پژوهشکده گیاهان دارویی