@ARTICLE{Sadat-Ebrahimi, author = {Hajimehdipoor, H and Sadat-Ebrahimi, SE and Amanzadeh, Y and Izaddoost, M and Givi, E and }, title = {Identification and Quantitative Determination of 4-Hydroxyisoleucine in Trigonella foenum-graecum L. from Iran}, volume = {9}, number = {33}, abstract ={Background: The seeds of Trigonella foenum-graecum L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine. Objective: In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated. Methods: Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique. Results: The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran. Conclusion: According to the effects of 4-hydroxyisoleucine such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders. }, URL = {http://jmp.ir/article-1-505-fa.html}, eprint = {http://jmp.ir/article-1-505-fa.pdf}, journal = {Journal of Medicinal Plants}, doi = {}, year = {2010} }