TY - JOUR T1 - Purification, identification, and standardization of silybin A & B composition from Silybum marianum (L.) Gaertn. TT - خالص‌سازی، شناسایی و استانداردسازی ترکیبات سیلی‎بین آ و ب از گیاه خارمریم JF - jmpir JO - jmpir VL - 21 IS - 81 UR - http://jmp.ir/article-1-3113-en.html Y1 - 2022 SP - 1 EP - 11 KW - Silybum marianum KW - Silybin A KW - B KW - Column chromatography KW - Diaion HP-20 KW - Sephadex LH-20 KW - HPLC KW - NMR N2 - Background: Silybum marianum (L.) Gaertn. (Milk thistle) is a perennial herb with medicinal properties. The seeds of these plants contain silymarin compounds with flavonolignan structure and antioxidant, anti-inflammatory and hepatoprotective effects. The major bioactive constituent of S. marianum is silybin A and B. It is used in the treatment of various liver conditions and exhibits high anti-tumor promoting activity. Objective: The purpose of this study was to purify, identify, and standardize of silybin A and B from the seeds extract of Silybum marianum. Methods: At first, the milk thistle seeds were defatted with hexane and then extracted with methanol as solvent. Isolation and further purification of silybin A and B was carried out by column chromatography using Diaion HP-20 resin, silica gel and Sephadex LH-20 as stationary phase, respectively. 1H-NMR and 13C-NMR techniques were used to identify these compounds. Finally, the HPLC method has been used to standardize. Results: 1H-NMR and 13C-NMR techniques characterized the structure of silybin A and B extracted from Silybum marianum L. and standardization and determination of their purity was performed using HPLC. Conclusion: Our proposed system presented significant advantages in increasing efficiency and reducing cost, and the diastereoisomers of silybin A and silybin B in silymarin were successfully isolated with high purities. M3 10.52547/jmp.21.81.1 ER -