%0 Journal Article %A Hajihossein, Reza %A Eslamirad, Zahra %A Rafiei, Fatemeh %A Naderi, Gholamreza %A Assadi, Morteza %T Anti-Acanthamoeba effect of Camellia sinensis extract (black and green tea) in vitro %J Journal of Medicinal Plants %V 19 %N 73 %U http://jmp.ir/article-1-2346-en.html %R 10.29252/jmp.1.73.163 %D 2020 %K Acanthamoeba, Camellia sinensis, Black tea, Green tea, Complementary therapy, Traditional medicine, %X Background: Acanthamoeba is a resistant protozoan that causes severe diseases, such as GAE and CAK. Because many medications are ineffective on the parasite, the quest to find alternative drugs is in progress. Objective: This research was aim ed to assess the performance of the black and green tea extracts on Acanthamoeba. Methods: The clinical isolate of Acanthamoeba was cultured on non-nutrient agar plates. The black and green tea extracts were prepared via maceration, dried by rotary evaporation, and stored at 4 °C. The main component of the extracts (caffeine) was recognized using GC-MS analysis. The effects of three concentrations of black and green tea extracts were evaluated on the parasite and compared with the control and current drug. Results: Black and green tea extracts have lethal effects on Acanthamoeba cysts and the performance was more than that shown by the control and the current anti-keratitis drug. Moreover, the effectiveness of the tea extracts was tim e- and dose-dependent (P < 0.0001). There was no significant differences between the performance of black and green tea (P > 0.05). Conclusion: Black and green tea extracts demonstrated the potential to inhibit the Acanthamoeba parasite, but the use of tea extract in clinical applications requires further study. %> http://jmp.ir/article-1-2346-en.pdf %P 163-169 %& 163 %! %9 Research %L A-10-2152-1 %+ Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran %G eng %@ 2717-204X %[ 2020