TY - JOUR JF - jmpir JO - J. Med. Plants VL - 17 IS - 65 PY - 2018 Y1 - 2018/3/01 TI - Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method TT - Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method N2 - Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids. Objective: To evaluate the hairy root culture as a novel method for thebaine production. Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT). Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers. Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers. SP - 73 EP - 82 AU - Qavami, N AU - Azizi, M AU - Yazdian, F AU - Qaderi, A AU - Nemati, H AD - Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran KW - Papaver bracteatum KW - Lindl. Hairy root KW - Sonication-assisted KW - Agrobacterium-mediated transformation KW - Thebaine UR - http://jmp.ir/article-1-2087-en.html ER -