year 20, Issue 79 (8-2021)
J. Med. Plants 2021, 20(79): 14-23 |
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Research code: 95-01-56-31529
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Tofighi Z, Shahpar Y, Taheri A, Tavakoli S, Asatouri R, Eftekhari M, et al . Identification of isoflavonoids in antioxidant effective fraction of Arum rupicola Boiss. leaves. J. Med. Plants 2021; 20 (79) :14-23
URL: http://jmp.ir/article-1-3067-en.html
URL: http://jmp.ir/article-1-3067-en.html
Z Tofighi1 
, Y Shahpar2 , AR Taheri3 , S Tavakoli4 , R Asatouri5 , M Eftekhari6 , M Vazirian2 , MR Shams Ardekani2 , SN Sadati Lamardi * 7
, Y Shahpar2 , AR Taheri3 , S Tavakoli4 , R Asatouri5 , M Eftekhari6 , M Vazirian2 , MR Shams Ardekani2 , SN Sadati Lamardi * 7
1- Medicinal Plants Research Center, and Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2- Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Medicinal Chemistry, Pharmaceutical Branch Islamic Azad University, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, IranDepartment of Organic and Phyto Chemistry, Faculty of Chemistry, Kharazmi University, Tehran, Iran
5- Department of Biology, Science and Research Branch Islamic Azad University, Tehran, Iran
6- Department of Pharmacognosy and Pharmaceutical Biology, Faculty of Pharmacy,Kermanshah University of Medical Sciences, Kermanshah, Iran
7- Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran , n_sadati@sina.tums.ac.ir
2- Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Medicinal Chemistry, Pharmaceutical Branch Islamic Azad University, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, IranDepartment of Organic and Phyto Chemistry, Faculty of Chemistry, Kharazmi University, Tehran, Iran
5- Department of Biology, Science and Research Branch Islamic Azad University, Tehran, Iran
6- Department of Pharmacognosy and Pharmaceutical Biology, Faculty of Pharmacy,Kermanshah University of Medical Sciences, Kermanshah, Iran
7- Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran , n_sadati@sina.tums.ac.ir
Abstract: (2262 Views)
Background: Arum rupicola Boiss. (Araceae Family) is used by the native people of southern areas of Iran as a soup called "kardeh soup". Several flavonoids and phenol compounds have been identified from Arum species. Objective: The aim of this study was to evaluate antioxidant effect and total phenol contents as well as cytotoxic activity of the leaves of A. rupicola. Methods: Antioxidant activity of total methanol extract and fractions including n-hexane, chloroform, ethyl acetate and water residue were evaluated using FRAP and DPPH methods. Total phenol content was measured using Folin-Ciocalteu method. Cytotoxic activity of the extract and fractions were investigated against human breast cancer MCF-7, MDA-MB-231, and T47D cell lines by MTT assay. Further phytochemical isolation was done on the water residue using column chromatography. Results: According to the results, water residue showed the lowest IC50 value (186.7 µg/ml) and the total methanol extract showed the most antioxidant power (163.62 mmol FeSO4/100 g extract) and phenol content (135 µmol Gallic acid/g extract). The hexane fraction also showed the highest cytotoxic effect against MCF-7 breast cancer cell line with IC50 equal to 118.9 μg/ml. Phytochemical analysis of the water residue resulted in isolation and identification of three isoflavonoids named orobol, genistein and genistein 8-c-glucoside. Conclusion: Based on the identification of isoflavonoid compounds in this plant, its ability to be used as a phytoestrogenic supplement can be considered in future studies.
Type of Study: Research |
Subject:
Pharmacognosy & Pharmaceutics
Received: 2021/03/10 | Accepted: 2021/07/3 | Published: 2021/09/1
Received: 2021/03/10 | Accepted: 2021/07/3 | Published: 2021/09/1
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