year 20, Issue 78 (5-2021)                   J. Med. Plants 2021, 20(78): 26-35 | Back to browse issues page

Research code: 189
Ethics code: IR.SBMU.RETECH.REC.1396.153


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1- Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3- Traditional Medicine and Materia Medica Research Center and Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5- Department of Iranian Traditional Medicine, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
6- Traditional Medicine and Materia Medica Research Center and Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , rchoopani@sbmu.ac.ir
Abstract:   (2665 Views)
Background: Trigonella foenum-graecum L. (fenugreek) seeds have a long history of treating allergic asthma known as “Rabv” in Persian medicine. The protective effects of fenugreek on asthma have been noted in Persian medicine texts. Objective: The aim of the present study was to investigate the protective effects of aqueous fenugreek seed extract (AFSE) on the reduction of Th2 cytokines via decreasing the levels of mRNA expression of Th2 cytokines in bronchoalveolar lavage fluid (BALF). Methods: 28 female Balb/c mice were divided into four groups of seven animals. Negative and positive control groups received Phosphate-buffered saline and ovalbumin (OVA), respectively. The remaining two groups were firstly sensitized with OVA to induce asthma and then received Theophylline and AFSE. Thereafter, the mRNA expressions of Th2-type interleukins (IL-4, IL-5, and IL-13) and mucin5 as well as the concentrations of IL-5, IL-13, and IL-33 in BALF samples were measured and pathological alterations of lung tissues were analyzed. Results: AFSE treatment of Balb/c mice significantly decreased the number of eosinophils, the mRNA expressions of IL-4, IL-5, IL-13, and mucin5 and the concentrations of IL-5, IL-13, and IL-33 in their BALF specimens. It also considerably prevented peribronchial and perivascular inflammations, mucus hypersecretion, and goblet cell hyperplasia in lung tissues in comparison to OVA-sensitized mice. Conclusion: The present study demonstrated that the aqueous fenugreek seeds extract could be an alternative medication for the treatment of allergic asthma.
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Type of Study: Research | Subject: Medicinal Plants
Received: 2020/05/4 | Accepted: 2021/04/21 | Published: 2021/06/1

References
1. Agrawal DK and Shao Z. Pathogenesis of allergic airway inflammation. Curr Allergy Asthma Rep. 2010; 10(1): 39-48. [DOI:10.1007/s11882-009-0081-7]
2. Shamshuddin NSS and Zohdi RM. Gelam honey attenuates ovalbumin-induced airway inflammation in a mice model of allergic asthma. J Tradit Complement Med. 2018; 8(1): 39-45. [DOI:10.1016/j.jtcme.2016.08.009]
3. Fahy JV. Type 2 inflammation in asthma-present in most, absent in many. Nat. Rev. Immunol. 2015; 15(1): 57-65. [DOI:10.1038/nri3786]
4. Barnes P and Adcock I. How Do Corticosteroids Work in Asthma? Ann Intern Med. 2003; 139: 359-370. [DOI:10.7326/0003-4819-139-5_Part_1-200309020-00012]
5. Piao CH, Bui TT, Song CH, Shin HS, Shon DH and Chai OH. Trigonella foenum-graecum alleviates airway inflammation of allergic asthma in ovalbumin-induced mouse model. Biochem. Biophys. Res. Commun. 2017; 482(4): 1284-1288. [DOI:10.1016/j.bbrc.2016.12.029]
6. Bae MJ, Shin HS, Choi DW and Shon DH. Antiallergic effect of Trigonella foenum-graecum L. extracts on allergic skin inflammation induced by trimellitic anhydride in BALB/c mice. J. Ethnopharmacol. 2012; 144(3): 514-522. [DOI:10.1016/j.jep.2012.09.030]
7. Srinivasan K. Fenugreek (Trigonella foenum-graecum): A review of health beneficial physiological effects. Food Rev. Int. 2006; 22(2): 203-224. [DOI:10.1080/87559120600586315]
8. Nathiya S, Durga M and Devasena T. Therapeutic role of Trigonella foenum-graecum [fenugreek] - a review. Int. J. Pharm. Sci. Rev. Res. 2014; 27(2): 74-80.
9. Yadav UCS and Baquer NZ. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharm. Biol. 2014; 52(2): 243-254. [DOI:10.3109/13880209.2013.826247]
10. Neelakantan N, Narayanan M, De Souza RJ and Van Dam RM. Effect of fenugreek (Trigonella foenum-graecum L.) intake on glycemia: A meta-analysis of clinical trials. Nutr. J. 2014; 13(7): 1-11. [DOI:10.1186/1475-2891-13-7]
11. Adedapo AA, Ofuegbe SO and Soetan KO. Pharmacologic and Medicinal Properties of Fenugreek (Trigonella foenum-graecum L.). American J. Social Issues and Humanities 2014: 13-20.
12. Emtiazy M, Oveidzadeh L, Habibi M and Molaeipour L. Investigating the effectiveness of the Trigonella foenum-graecum L. (fenugreek) seeds in mild asthma: A randomized controlled trial. Allergy, Asthma Clin. Immunol. 2018; 14(1): 1-8. [DOI:10.1186/s13223-018-0238-9]
13. Akbari S, Abdurahman NH, Yunus RM, Alara OR and Abayomi OO. Extraction, characterization and antioxidant activity of fenugreek (Trigonella-foenum graecum) seed oil. Mater. Sci. Energy Technol. 2019; 2(2): 349-355. [DOI:10.1016/j.mset.2018.12.001]
14. Haravi Q b. YAN. The Irshad Al-Ziraa. Amirkabir, Tehran.; 1977. p. 101.
15. Jorjani SI. Al- Aghraz al- Tebbieh va al- Mabahes al-Alayieh [Medical Pursuits. Tehran: Tehran University Press; 2005. p. 592.
16. Mohamadi N, Sharififar F, Pournamdari M, Ansari M. A Review on Biosynthesis, Analytical Techniques, and Pharmacological Activities of Trigonelline as a Plant Alkaloid. Journal of Dietary Supplements 2018; 15(2):207-22. [DOI:10.1080/19390211.2017.1329244]
17. Niknam R, Mousavi M, and Kiani H. New Studies on the Galactomannan Extracted from Trigonella foenum-graecum (Fenugreek) Seed: Effect of Subsequent Use of Ultrasound and Microwave on the Physicochemical and Rheological Properties. Food Bioprocess Technol. 2020; 13(5): 882-900. [DOI:10.1007/s11947-020-02437-6]
18. British pharmacopoeia. London: Stationery Office. 2015, IV-186.
19. Athari SS, Pourpak Z, Folkerts G, Garssen J and Moin M.Conjugated Alpha-Alumina nanoparticle with vasoactive intestinal peptide as a Nano-drug in treatment of allergic asthma in mice. Eur. J. Pharmacol. 2016; 791: 811-820. [DOI:10.1016/j.ejphar.2016.10.014]
20. Irani M, Choopani R, Esmaeili S, Dargahi T, Athari SM AS. Effect of nettle seed on immune response in a murine model of allergic asthma. Rev. Fr. Allergol. 2020; 60 (5): 417-422. [DOI:10.1016/j.reval.2020.03.007]
21. Van Hoecke L, Job ER, Saelens X and Roose K. Bronchoalveolar lavage of murine lungs to analyze inflammatory cell infiltration. J. Vis. Exp. 2017; 123: 1-8. [DOI:10.3791/55398]
22. Choi J, Choi BK, Kim Js, Lee JW, Park HA and Ryu HW. Picroside II attenuates airway inflammation by downregulating the transcription factor GATA3 and Th2-related cytokines in a mouse model of HDM- induced allergic asthma. PloS one 2016; 11(11): 1-15. [DOI:10.1371/journal.pone.0167098]
23. Piao CH, Bui TT, Song CH, Shin HS, Shon DH and Chai OH. Trigonella foenum-graecum alleviates airway inflammation of allergic asthma in ovalbumin-induced mouse model. Biochem. Biophys. Res. Commun. 2017; 482(4): 1284-1288. [DOI:10.1016/j.bbrc.2016.12.029]
24. Del Hierro JN, Reglero G and Martin D. Chemical characterization and bioaccessibility of bioactive compounds from saponin‐rich extracts and their acid‐hydrolysates obtained from fenugreek and quinoa. Foods 2020; 9(9). [DOI:10.3390/foods9091159]
25. Ojiako CM, Okoye EI, Oli AN, Ike CJ, Esimone CO and Attama AA. Preliminary studies on the formulation of immune stimulating complexes using saponin from Carica papaya leaves. Heliyon. 2019; 5(6): e01962. [DOI:10.1016/j.heliyon.2019.e01962]
26. Betoret A V, Ruiz AD and Landivar O. System and method for energy optimization in photovoltaic generators. US Pat. 2012; 118(11): 3546-3556.

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