2024-03-28T22:14:54+03:30 http://jmp.ir/browse.php?mag_id=73&slc_lang=en&sid=1
73-1905 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 The Efficacy of Herbal Medicines for Treatment of Hot Flash: A Systematic Review L Kashani F Khajuei Kermani S Akhondzadeh s.akhond@neda.net Background: Hot flashing is the most common symptoms experienced by women around the menopause time. The most common therapy for relief of menopausal symptoms is Hormone Replacement Therapy (HRT), but it has side effects. So, many women are interested in herbal medicines because of fear of side effect of hormone therapy. Objective: The aim of this study was assess the effectiveness of different herbal medicine on the hot flash in menopausal women in clinical trial. Methods: The database of Medline, Scopus, and Web of Science were searched to investigate randomized controlled clinical trials on the efficacy of herbal medicines on hot flash from Feb 1994 to Feb 2017. Results: Fifty nine randomized controlled trials found according to the inclusion criteria. The results demonstrating that different herbal medicines such as Glycine max, Cimicifuga racemose, Vitex agnus-castus, Glycyrrhiza glabra, Trigonella foenum-graecum, Humulus lupulus, Valeriana officinalis, Linum usitatissimum, Salvia officinalis, Pimpinella anisum, Trifolium pretense, Hypericum perforatum and Ginkgo biloba can have role in reduction of the hot flashes. Conclusion: Our study show that many herbal medicines can reduce the frequency and number of hot Flash but it needs more future studies to find reliable result. Hot flash Herbal medicine Menopause 2017 11 01 1 20 http://jmp.ir/article-1-1905-en.pdf
73-1626 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Herbs from Iranian Traditional Medicine for Reinforcement of Kidney and Prevention of Renal Atrophy Fatemeh Kolangi dr.kolaangi@gmail.com Z Memariani zmemariani@gmail.com N Gorji nargess_gorji@yahoo.com SA Mozaffarpur seyyedali1357@gmail.com R Moeini dr.moeini_90@yahoo.com R Saghebi rsaghebi@gmail.com M Mojahedi mortazamojahedy@gmail.c Background: Renal disorders including renal failure are common complications which affect the whole body system. The treatment methods in modern medicine seem to be inadequate in a large number of cases. Regarding the increasing interest of the community towards traditional and complementary medicine, the potential of Persian medicine (PM) in management of renal diseases, could be put to test in research programs in order to find possible effective treatment. Objective: The aim of this study is to introduce materia medica which are commonly used in PM for the purpose of protection and functional empowerment of the kidneys against destructive factors. Methods: This research is a literature-based study on effective material medica used in the treatment of two significant renal disorders; renal weakness and renal atrophy (Hozal). Six pharmaceutical references of Persian medicine from different historical periods were searched by certain keywords related to renal weakness and renal atrophy. Results: Fourteen materia medica related to the prevention or treatment of renal weakness and fifteen for renal atrophy were found. Poppy (Papaver somniferum), Amber and shellac are the most repeated medicines in the treatment of renal weakness; whereas fig (ficus carica), banana (musa sapientum) and hazelnut (corynus avelana) are mostly repeated for either prevention or treatment of renal atrophy. Conclusion: Medicinal plants represented by Persian medicine references could be a basis for experimental and clinical researches for drug development in the field of prevention or even treatment of acute or chronic renal failure. Materia medica Nephroprotective Persian Medicine Renal failure Renal weakness 2017 11 01 21 31 http://jmp.ir/article-1-1626-en.pdf
73-1907 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Changes in Nepetalactone Content and Biochemical traits of Catnip (Nepeta cataria L.) in Response to Induction of Biostimulants Compounds N Ozhan M Goldani HA Naghdi Badi A Mehrafarin M Parsa Background: The use of organic and biological stimulants at different stages of plant growth may increase growth and yield of plants in addition to reducing environmental stresses. Objective: The aim of this study was to determine the induction effect of various formulations of chitosan, humic acid, and nicgtric acid on nepetalactone content and biochemical traits in catnip. Methods: This study, which was based on a completely randomized design (CRBD), was conducted in the research greenhouse of Medicinal Plants Research Institute, ACECR. Treatments consisted of: control, citric acid, different concentrations of humic acid, dual combinatorial formulations of chitosan and citric acid, and triple combinatorial formulations of chitosan, citric acid, and humic acid. First, the roots of the transplants were treated before being transferred to the pot. Then, about 20 days after planting, treatments were sprayed on the plants three times – once every15 days. Results: Results showed that the induction of different formulations of humic acid, citric acid, and chitosan had significant effects on plant height (P≤0.05), the number of lateral branches, the number of leaves, dry weight of leaves, stems, and shoot, content of soluble sugar, phenols, tannins, flavonoids, and nepetalactone (P≤0.01). The highest amounts - in most morpho-physiological traits - were observed 400 ppm chitosan + 800 ppm humic acid + 400 ppm citric acid treatment. The maximum content of nepetalactone was obtained at 200 ppm chitosan + 800 ppm humic acid + 400 ppm citric acid. Conclusion: The use of biostimulants formulation including humic acid, citric acid, and chitosan had a significant positive effect on improving vegetative characteristics and especially on phytochemical traits of catnip (Nepeta cataria L.). Nepeta cataria L. Biostimulant Chitosan Citric acid Humic acid 2017 11 01 32 44 http://jmp.ir/article-1-1907-en.pdf
73-1403 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Effect of Five Weeks Circuit Resistance Training with Garlic Supplementation on Serum Levels of Adiponectin in Over Weight Female Zeynab Hamidnejad minoohamidnezhad.0082@yahoo.com Seyed Mohsen Avandi M.avandi@semnan.ac.ir Rohollah Haghshenas rhm@profs.semnan.ac.ir Abbas Pakdel pakdel1976@yahoo.com Background: Obesity and Metabolic Disorders is one of the risk factors for health in the world. Physical activity and herbal supplements is the research topic in the field of health and reducing obesity. Objective: The purpose of this study was to compare the effect of five weeks circuit resistance training with garlic supplementation on serum levels of adiponectin in over weight female. Methods: In a double-blind trial quasi-experimental design, 32 healthy overweight women were selected and randomly divided into following four groups: Placebo, supplement, training and placebo and training and supplement groups. The experimental subjects received circuit resistance training was performed three times a week. All groups were given 1000 mg garlic supplements or placebo for five weeks in two doses of 500 mg daily. Anthropometric indices, and blood sampling were measured before and after five weeks of circuit resistance training. Results: After five weeks, levels of adiponectin in serum increased in resistance training group with garlic with compared to other groups, but this increase was not statistically significant (p=0.123) also body weight did not show significant difference (P=0.99). Conclusion: Based on the results suggested that more research investigate effect of garlic supplementation with resistance exercise on weight loss and serum adiponectin levels and other indicators associated with obesity in obese women. Adiponectin Garlic supplement Resistance training 2017 11 01 45 57 http://jmp.ir/article-1-1403-en.pdf
73-1909 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Expression of Genes 1-deoxy-D-xylulose 5-phosphate Reductoisomerase (DXR), Sabinen Synthase (Tctps1) and α- terpineol Synthase (Tctps5) and its Relation to Thymol and Carvacrol Biosynthesis in Thymus vulgaris under Water Deficit Stress F Mirzaei F Fatehi A Ismaili A Yaghotipoor A Qaderi Ardeshir582008@gmail.com Background: Thymol and Carvacrol are the two important secondary metabolites from Thymus vulgaris that productions of them are controlled by genetic and environmental factors. Objective: Evaluation of Water stress effects on expression of three important genes of thymol and carvacrol biosynthetic pathway and also physiological and phytochemical properties of Thymus vulgaris. Methods: The present study was conducted in a randomized complete block design with 4 treatments and 3 replications in Institute of Medicinal Plants, ACECR, Karaj.  For this purpose, the treatments were selected as control (FC), 70% of FC, 40% of FC, and 20% of FC. Gene expression was studied using real-time PCR method, and HPLC was applied to essence analysis. Also physiological characteristic including chlorophyll content, relative water content, electrolyte leakage percentage and carotenoids content were analyzed. Results: The results revealed that water stress significantly influenced the gene expression (P≤ 0.05). The highest gene expression of DXR and Tctps5 was observed in 70% of FC, while the highest one of TvTPS1 was recorded in 40% of FC. Moreover, the maximum thymol and carvacrol was found in 70% of FC. In other hand the highest chlorophyll content and corotenoids content were obtained in 100% and 70% of FC respectively. Also there were not significant differences between treatments for  relative water content and electrolyte leakage percentage. Conclusion: The 70% of FC through influencing of genes in the firs and last of MEP pathway increased thymol and carvacrol production.   Thymus vulgaris Gene Expression water deficit Photosynthesis Pigments thymol carvacrol 2017 11 01 58 70 http://jmp.ir/article-1-1909-en.pdf
73-1402 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Synchronic Effect of Curcumin and swimming training on depression in morphine addicted male mice L Elhampour laleh_elhampour@yahoo.com MA Azarbayjani m__azarbayjani@iauctb.ac.ir M Nasehi nasehi@iricss.org M Piri M.peeri@gmail.com MR Zarindast zarinmr@ams.ac.ir Introduction: There is need to more research because of extent drug trade in country and diversity of proposal cures such as non pharmacological ways including exercise and herbal medicine. The aim of the study: The aim of this study was study of synchronic effect of Curcumin and swimming training on depression in morphine addicted male mice. Methods: In an experimental research, 40 Albino NMRI male mice with 19- 21 gr were selected as subjects and they were divide randomly to care groups of control, morphine, swim training, Curcumin, swim training+ Curcumin. Subjects swam 60 min/day, 5 days/week for 1 week with receiving Curcumin. They were taken morphine (2.5 mg/ml/kg) for 5 days. Therefore FST were done and after 10 days this test was taken again. Results: training increased swimming time, but it had no significant effect on immobility and climbing. At this period, Curcumin supplementation caused increasing the time of climbing and no alteration in immobility and swimming times. Curcumin supplementation with swim training increased immobility time and decreased swimming time, but it had no significant effect on climbing time. Conclusion: the results of this research showed that morphine consumption didn’t cause depression. Also, combination of Curcumin supplementation and swim training hasn’t caused depression decreasing in morphine consumption term. Swimming Curcumin Forced Swim Test Depression 2017 11 01 71 82 http://jmp.ir/article-1-1402-en.pdf
73-1530 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Hypoglycemic Interaction Effects of 4 and 6 Weeks Swimming Training and Aloe barbadensis Hydroalcoholic Extract in Streptozotocin Induced Diabetic Rats M Shamshiri mehrabani1000@gmail.com SA Hosseini alihoseini_57@yahoo.com Background: Exercise and nutrition are basic pillars of taking care and management of diabetes. Objective: Aim of present study was to review the hypoglycemic interaction effects of swimming and Aloe barbadensis in diabetic rats. Methods: 72 diabetic rats selected and divided in 9 groups (1) first week control, (2) fourth week control, (3) sixth week control, (4) 4 weeks Aloe barbadensis, (5) 6 weeks Aloe barbadensis, (6) 4 weeks swimming, (7) 6 weeks swimming, (8) 4 weeks swimming with Aloe barbadensis, (9) 6 weeks swimming with Aloe barbadensis. For review the effect of diabetes induction 24 healthy rats selected and divided in 3 groups (10) first week control, (11) fourth week control and (12) sixth week control. Groups 6 and 8 swam for 4 weeks and rats of groups 7 and 9 swam for 6 weeks, 5 sessions per week and 30 minutes per session also groups 4, 5, 8 and 9 received 100 mg/kg Aloe barbadensis daily. For statistical analysis of data used one-way ANOVA, two-way ANOVA and bonferroni post hoc tests (P≤0.05). Results: Diabetes induction has significant effect on increase of glucose and insulin resistance and reduction of insulin in diabetic rats (P<0.05), swimming training, Aloe barbadensis and combination of swimming and Aloe barbadensis have significant effect on reduction of glucose and insulin resistance (P<0.05) also 6 weeks swimming rather than 4 weeks and 6 weeks Aloe barbadensis rather than 4 weeks have more effect on reduction of glucose and insulin resistance (P<0.05). Conclusion: 4 and 6 weeks swimming and Aloe barbadensis have hypoglycemic interaction effects in diabetic rats. Swimming Training Aloe barbadensis Diabetes Glycemic Indexes 2017 11 01 83 92 http://jmp.ir/article-1-1530-en.pdf
73-1553 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Phytochemical and Morphophysiological Responses of Dill (Anethum graveolens L.) to Foliar Application of Potassium Sulfate and Methanol Biostimulant A Mehrafarin HA Naghdi Badi M Mirzai Motlagh M Salehi mhtb.salehi@gmail.com M Ghiasi Yekta Background: The application of methanol biostimulant and potassium sulfate with reducing of photorespiration and transpiration in C3 plants, increasing the absorption of water and food, and biosynthesis of amino acids can have a direct or indirect effect on the growth and metabolic index of plants. Objective: This study was directed to investigate the effect of methanol and potassium sulfate foliar application on morpho-physiological and phytochemical changes in dill. Methods: This study was conducted as a factorial experiment based on randomized complete block design at institute of medicinal plants. Experimental treatments included foliar application of methanol at 3 levels (0, 20, 40 v/v%) and potassium sulfate at 4 levels (0, 1, 1.5, 2 g.L-1). Results: Methanol application increased the leaf and stem dry weight, and inflorescence fresh weight. Foliar application of different levels of potassium sulfate also increased the plant height, leaf dry weight, and inflorescence and shoot fresh weight. The use of methanol was not effective on the amount of essential oil in the dill. But, increased the amount of α-pienene. Low concentration of methanol increased the content of thymol, myrcene, α-phellandrene, and p-cymenen. So, increasing the concentration of methanol reduced their amount. Conclusion: Phytochemical and morphophysiological traits of dill were increased with application of methanol and potassium sulfate. As, the highest amount of leaf and stem dry weight, inflorescence fresh weight, and α-pienene was obtained with 40% methanol application. Also, the use of 2 g.L-1 of potassium sulfate produced the highest amount of plant height, leaf dry weight, inflorescence and shoot fresh weight. Anethum graveolens L. Essential oil Foliar application Methanol Potassium sulfate 2017 11 01 93 108 http://jmp.ir/article-1-1553-en.pdf
73-1446 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Investigation of Antibacterial Activity of Pimpinella affinis Leaves Against E. coli and S. aureus and Separation of Active Fractions Yaghoub Sarrafi ysarrafi@umz.ac.ir Hadis Tavahodi Hossein Dehghan h.dehghan@shahed.ac.ir Background: Pimpinella affinis (Apiaceae family) is a vegetable in the north of Iran. Objective: This study was conducted to investigate antibacterial activity of Pimpinella affinis (against E. coli and S. aureus) and separate its active fractions. Methods: Antibacterial activities of n-hexane, ethyl acetate and methanol extracts of leaves of P. affinis (against E. coli and S. aureus) were measured using broth microdilution method in MIC. Subsequently, the ethyl acetate extract was separated in to 29 fractions using column chromatography and thin layer chromatography (TLC). Also, antibacterial activities of the fractions have been measured. Results: According to the results, among the extracts, ethyl acetate extract showed the best activities against E. coli and S. aureus (MICs=1.0 and 2.0 mg/ml, respectively). Also, among the obtained fractions from ethyl acetate extract, F3-F5, F8 and F9 showed best inhibitory activities (MIC=5.0 μg/ml, P<0.05) compared with that of the chloramphenicol (MIC=1.0 μg/ml, P<0.01). All of the results have p-values less than 0.05. Conclusion: The results showed that the Gram positive S. aureus is more sensitive to the extracts than the Gram negative E. coli. Also, partitioning of the ethyl acetate extract leaded to obtain antibacterial fractions (F3-F5, F8 and F9) with 200-fold more active than the ethyl acetate extract.   Pimpinella affinis Escherichia coli Staphylococcus aureus Chromatography 2017 11 01 109 115 http://jmp.ir/article-1-1446-en.pdf
73-1380 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Collection, identification and traditional usage of medicinal plants in Jiroft County Hossein Bibak hbibak@ujiroft.ac.ir Fatemeh Moghbeli hanjaee fmoghbeli916@gmail.com Background: Medicinal plants have been used for thousands of years as an important source of medicinal. Traditional botany offer valuable methods to finding new medicinal plants and herbal medicines. Objective: The aim of this study was to identify the important native medicinal plants used by traditional healers of Jiroft. Methods: The ethno-medicinal data of the plants were collected through field surveys, face-to-face interview with local herbalist and documentary studies. The traditional ethno-medicinal knowledge was recorded and for each species scientific name, local name, life form, therapeutic characteristic, used plant parts were aerial parts. Results: A total of 147 species of medicinal plants belonging to 132 genera in 48 families were recorded. In this study the most frequently used plant was as comforting, healing wounds, stomach tonic, painkiller and anti-inflammation and other use in traditional medicine that they can be exploited with careful planning and management. Conclusion: Prevalence and widespread use of medical herbs provide a context for identification of new treatment characteristics of plants in the region. Limited internal resources, development of employment plans based on cultivation and development of medicinal plants compatible with ecological conditions can lead to maximum productivity and provide a proper way to preserve these resources. Jiroft Medicinal plants Traditional use 2017 11 01 116 140 http://jmp.ir/article-1-1380-en.pdf
73-1915 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Phytochemical and Morpho-physiological changes lavender (Lavandula officinalis L.) in Response to different culture media R Joshaghani A Mehrafarin MR Labbafi Background: Since the essential oil of lavender (Lavandula officinalis L.) was used for the treatment of diseases related to the nervous system and rheumatism, phytochemical evaluation of this plant is essential especially in terms of cultivation and production. Objective: This study was conducted to investigate the effect of different culture media on the amount of essential components, chlorophyll content, nutrients, growth characteristics, and rooting of lavender. Methods: The experiment was done the basis of randomized complete blocks design (RCBD) in 10 treatments and 3 replications. Substrates and culture media for plant production in this study were M1; sand, M2; sand:perlite (2:1), M3; sand:peat-moss (2:1), M4; sand:vermicompost (2:1), M5; sand:perlite:peat-moss (2:1:1), M6; sand:perlite:vermicompost (2:1:1), M7; sand:peat-moss:vermicompost (2:1:1), M8; sand:vermicompost:peat-moss:perlite (2:1:1:2), M9; sand:vermicompost:peat-moss:perlite (4:1:1:2), and M10; sand:vermicompost:peat-moss:perlite (6:1:1:2). Results: Results showed that culture media had significant effect on the all characteristics of rooting, chlorophyll and nutrient content and essential oil components in lavender plants. Limonene, borneol, and camphor were the highest amount of essential oil compounds in lavender, respectively. The highest content of limonene and oxygenated monoterpenes in M1, rooting percentage and number of main root in M3, camphor and chlorophyll content and root dry weight in M5, borneol content in M8, and hydrocarbon monoterpenes in M10 were observed. Conclusion: The application of different culture media in the production and propagation of lavender in addition to changes in morpho-physiological characteristics of lavender can directly alter the properties of essential oil and phytochemical compounds. Limonene Borneol Peat-moss Perlite Vermicompost 2017 11 01 141 152 http://jmp.ir/article-1-1915-en.pdf
73-1916 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Comparison of Analgesic Effect of Valeriana officinalis and Mefenamic Acid on Primary Dysmenorrhea A Kazemian N Parvin M Delaram F Deris Background: Primary dysmenorrhea is a common and frequently disabling condition among women in adolescence and reproductive age. Based on results of large epidemiological studies, it is estimated that over a half of the population of young women suffers from dysmenorrhea. Some people are now seeking alternatives to conventional medicine such as herbal drugs. Objective: This study was designed to compare the analgesic effect of Valeriana officinalis with Mefenamic acid in treatment of primary dysmenorrhea. Methods: A randomized, controlled trial was undertaken among 39 female aged 16 to 42 who suffered from primary dysmenorrhea referred to gynecology clinic in Hajar hospital, Shahrekord, Iran.  The participants were randomly divided into two groups: Valeriana officinalis (n=18) and Mefenamicacid (n=21). In intervention group, the patients took350mg  Valerian three times a day, and in mefenamic group, the patients took 250 mg Mefenamic three times a day for three days(for three cycles), starting from the onset of bleeding or pain. Participants were followed for three cycles. Main outcome measures was the mean of pain severity at 3 months which recorded by Visual Analogue Scale. Results: There were no significant differences in age, menstural duration, menstural interval, and pain severity score at the beginning of the study in two groups (P>0.05). After the intervention, findings of the study revealed statistically significant reductions in mean of pain score in Valeriana officinalis (P<0.001) and Mefenamic acid (P<0.001) groups, but this reduction had not significant differences between two groups (P>0.05). Conclusion: Results shows that valerian has positive effect on treatment of primery dysmenorrheal. More clinical trials are needed to establish the efficacy of Valeriana officinalis in primary dysmenorrhea. Valeriana officinalis Mefenamic acid Primary Dysmenorrhea 2017 11 01 153 159 http://jmp.ir/article-1-1916-en.pdf
73-1397 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 comparison of differnt extraction methods for the extraction of bioactive components of Senna alexandrina Noora Enayati noora.enayati@ut.ac.ir Reza Ghafarzadegan reza.ghafary@yahoo.com Reza Hajiaghaee rhajiaghaee@yahoo.com Mehdi Vazirian vazirian_m@sina.tums.ac.ir Background and Purpose: Senna leaf is among the category stimulant laxatives. The laxative effect of this medicinal plant refers to sinusoids, aloe emodin and dynatron within it that all of them are among anthraquinone derivatives used for the treatment of constipation, discharge of the intestine before surgery or abdomen surgeries and tests. One of the most important issues raised in the extraction of active ingredients in the natural medicinal sources, is in the analysis and industry sectors, type and method of extraction of these substances to increase efficiency and extraction of these substances. This study was conducted to compare different extraction methods of the plant of Senna and to optimize the extraction of the active substance of B sinusoid using the statistical methods of response surface. Methods: For the extraction of Senna extract, various extraction methods, including maceración, dynamic maceración (stirrer), ultrasound waves, microwaves and Soxhlet with 96 and 60% ethanol solvents were used at different times. The best method with considering the weight means of the extract and active substance of B sinusoid was selected. In the next phase, using the statistical method of response surface, the amount of active substance of B sinusoid was optimized. Results: The results of ANOVA showed that with considering the weight mean of the extract and amount of B sinusoid, stirrer method is the most suitable method for the extraction of Senna in the industrial and medicinal extraction. The optimization results for the best extraction method using the response surface, according to the influential variables showed that when accessing the maximum amount of the active substance (3.009%) occurs that temperature, time, the ratio of substance to solvent and filter are 84°C, 119.7 minutes, 0.04 and 29.7 micron, respectively. Conclusion: Finally, it can be concluded that using active maceración extraction method (stirrer) is suggested as one of the best method for the extraction of Senna extract in industry and the output efficiency of the active substance of B sinusoid under the impact of the introduced optimized conditions will be at the maximum level. Senna alexandrina Extraction Stirrer B Sinusoid Response Surface Method 2017 11 01 160 169 http://jmp.ir/article-1-1397-en.pdf
73-1918 2024-03-28 10.1002
Journal of Medicinal Plants J. Med. Plants 2717-204X 2717-2058 10.61186/jmp 2017 16 64 Silver Nanoparticles Synthesis Using Artemisia turcomanica Ethanolic Extract and Study of Anti-proliferative Effects on Cell Line of Gastric Cancer B Mousavi F Tafvizi farzanehtafvizi54@gmail.com S Zaker Bostanabad Background: The use of plants for the synthesis of nanoparticles, because of not using pollutants and toxins, is considered an eco-friendly method. Objective: The purpose of this study is silver nanoparticles biosynthesis using Artemisia turcomanica extract and evaluating the toxicity of synthesized silver nanoparticles on gastric cancer cell line (AGS). Methods: For synthesizing silver nanoparticles, the leaf extract of Artemisia turcomanica was used. Phytomorphology of bio silver nanoparticles using Transmission Electron Microscopy (TEM) and Scanning Electron Microscope (SEM) analysis was evaluated. Cytotoxicity effects of bio nanoparticles in two cell lines of gastric cancer (AGS) and fibroblast normal cell (L-929) in concentrations of 3, 5, 12.5, 25, 50, and 100 µg/ml were evaluated in 24 hours by MTT assay. Apoptosis was analysed by flocytometry. Results: The IC50 value for silver nanoparticles was 4.88 µg/ml during 24 h on AGS cell line, which was significant comparing to the control group. Also the IC50 value for silver nanoparticles in 24 hours for normal cells was 14.56 µg/ml. Apoptosis was detected in test group which was more than necrosis. A significant difference was observed between apoptosis and necrosis. Conclusion: In this study the extract of Artemisia turcomanica was used for the first time for biosynthesis of silver nanoparticles and results suggest that synthesized silver nanoparticles have cytotoxicity and apoptosis induction effect on gastric cancer cells. Artemisia turcomanica Biological nanosilver Cytotoxicity 2017 11 01 170 182 http://jmp.ir/article-1-1918-en.pdf