en
jalali
1396
5
1
gregorian
2017
8
1
16
63
online
1
fulltext
en
An Overview on Two Valuable Natural and Bioactive Compounds, Thymol and Carvacrol, in Medicinal Plants
An Overview on Two Valuable Natural and Bioactive Compounds, Thymol and Carvacrol, in Medicinal Plants
.Thymol (2-isopropyl-5-methylphenol), and carvacrol (5-isopropyl-2-methylphenol) are the main components of the essential oils of some Laminaceae,Verbenaceae and Ranunculaceae members such as oregano, thyme, savory, etc. The formation of thymol and carvacrol is thought to involve hydroxylation of γ-terpinene and p-cymene precursors. They are produced by the plant species as a chemical defense mechanism upon exposure to pathogens, pests, herbivores, or environmental stresses. Accordingly, the potent antimicrobial and fungi toxic properties of these compounds against various plant pathogens have been demonstrated. They have antibacterial, antifungal, insecticidal, and anti-oxidative properties which are the basis for the wide use of these compounds in the cosmetic, food, and pharmaceutical industries.
Thymol (2-isopropyl-5-methylphenol), and carvacrol (5-isopropyl-2-methylphenol) are the main components of the essential oils of some Laminaceae,Verbenaceae and Ranunculaceae members such as oregano, thyme, savory, etc. The formation of thymol and carvacrol is thought to involve hydroxylation of γ-terpinene and p-cymene precursors. They are produced by the plant species as a chemical defense mechanism upon exposure to pathogens, pests, herbivores, or environmental stresses. Accordingly, the potent antimicrobial and fungi toxic properties of these compounds against various plant pathogens have been demonstrated. They have antibacterial, antifungal, insecticidal, and anti-oxidative properties which are the basis for the wide use of these compounds in the cosmetic, food, and pharmaceutical industries.
Thymol, Carvacrol, Biological activity, Biosynthesis pathway, Terpenes
Thymol, Carvacrol, Biological activity, Biosynthesis pathway, Terpenes
1
32
http://jmp.ir/browse.php?a_code=A-10-44-106&slc_lang=en&sid=1
2016/06/29
1395/4/9
2016/12/4
1395/9/14
HA
Naghdi Badi
Medicinal Plants Research Center, Institute of Medicinal Plants, Karaj, Iran
حسنعلی
نقدیبادی
Naghdibadi@yahoo.com
00319475328460041300
00319475328460041300
No
Medicinal Plants Research Center, Institute of Medicinal Plants, Karaj, Iran
M
Abdollahi
Science and Research Branch, Islamic Azad University, Department of Horticultural Tehran, Iran
محمد
عبدالهی
00319475328460041301
00319475328460041301
No
Science and Research Branch, Islamic Azad University, Department of Horticultural Tehran, Iran
A
Mehrafarin
Medicinal Plants Research Center, Institute of Medicinal Plants, Karaj, Iran
علی
مهرآفرین
a.mehrafarin@gmail.com
00319475328460041302
00319475328460041302
Yes
Medicinal Plants Research Center, Institute of Medicinal Plants, Karaj, Iran
M
Ghorbanpour
Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
منصور
قربانپور
00319475328460041303
00319475328460041303
No
Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
SM
Tolyat
Medicinal Plants Research Center, Institute of Medicinal Plants, Karaj, Iran
سیدمجید
تولیت
Toliat@acecr.ac.ir
00319475328460041304
00319475328460041304
No
Medicinal Plants Research Center, Institute of Medicinal Plants, Karaj, Iran
A
Qaderi
Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran
اردشیر
قادری
00319475328460041305
00319475328460041305
No
Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran
M
Ghiaci Yekta
Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran
مونا
غیاثی یکتا
00319475328460041306
00319475328460041306
No
پژوهشکده گیاهان دارویی جهاد دانشگاهی
en
Antioxidant Properties of Pistacia khinjuk Accelerate Healing of the Experimental Achilles Tendon Injury in Rabbits
Antioxidant Properties of Pistacia khinjuk Accelerate Healing of the Experimental Achilles Tendon Injury in Rabbits
Background: Delayed tendon healing and tendon adhesions are still found to be among the complications that occur most often after tendon repair. Pistacia khinjuk is one of the three species of Pistacia growing in Iran.
Objective: In this study, the role of local injection of ethanolic extract of P. khinjuk was evaluated in healing of the experimental Achilles tendon injury in rabbits.
Methods: Nine adult male white New Zealand rabbits were anesthetized and a partial thickness tenotomy was created on both hindlimbs. P. khinjuk extract and normal saline were injected daily to the treatment and control groups, respectively for three days post-operatively.
Results: Based on the findings, on days 14, 28 and 42 after the injury, the healing rate in
P. khinjuk treated groups were higher than control groups in tendon sections significantly
(P ≤ 0.05). In summary, results of this study showed that the application of P. khinjuk extract can improve the healing process of damaged Achilles tendon in rabbits.
Conclusion: Significant differences between the treatment and control groups in the current study suggest that the application of P. khinjuk extract can improve the healing process of damaged Achilles tendon.
Background: Delayed tendon healing and tendon adhesions are still found to be among the complications that occur most often after tendon repair. Pistacia khinjuk is one of the three species of Pistacia growing in Iran.
Objective: In this study, the role of local injection of ethanolic extract of P. khinjuk was evaluated in healing of the experimental Achilles tendon injury in rabbits.
Methods: Nine adult male white New Zealand rabbits were anesthetized and a partial thickness tenotomy was created on both hindlimbs. P. khinjuk extract and normal saline were injected daily to the treatment and control groups, respectively for three days post-operatively.
Results: Based on the findings, on days 14, 28 and 42 after the injury, the healing rate in
P. khinjuk treated groups were higher than control groups in tendon sections significantly
(P ≤ 0.05). In summary, results of this study showed that the application of P. khinjuk extract can improve the healing process of damaged Achilles tendon in rabbits.
Conclusion: Significant differences between the treatment and control groups in the current study suggest that the application of P. khinjuk extract can improve the healing process of damaged Achilles tendon.
Pistacia khinjuk, Achilles tendon, Antioxidant, Rabbit
,Pistacia khinjuk, Achilles tendon, Antioxidant, Rabbit
33
42
http://jmp.ir/browse.php?a_code=A-10-397-4&slc_lang=en&sid=1
2016/06/292017/02/26
1395/12/8
2016/12/42017/07/25
1396/5/3
HR
Moslemi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran
حمیدرضا
مسلمی
moslemi1357@gmail.com
00319475328460041307
00319475328460041307
Yes
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran
Hossein
Fazel
Graduated from Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
حسین
فاضل
hfazel@yahoo.com
00319475328460041308
00319475328460041308
No
Graduated from Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
en
Traditional uses of some medicinal plants in Gastrointestinal tract treatment in east - Mazandaran (Iran)
Traditional uses of some Medicinal Plants in Gastrointestinal tract Treatment in East - Mazandaran (Iran)
Background: Iran has a long history of traditional medicine and medicinal plants use in Middle East. Many ethnics in several parts of Iran use medicinal plants for their illness.
Objective: Our aims were to identify which medicinal plants in the East- Mazandaran used for gastro intestinal problems and how the indigenous people treat their gastro intestinal problems.
Methods: An investigation was carried out for a period of 2 years (from 2014 to 2015) in the area of East- Mazandaran. A questionnaire was prepared through face-to-face interviews. In order to collect detailed information we visited the people who had knowledge of medicinal plants and identify their collected dried medicinal plants. Persian and local name of the plants, their utilized parts and preparation methods were investigated and recorded.
Results: After ethnobotanical study in the East-Mazandaran region we recorded 20 species belong to 10 family of medicinal plant in this area. The most common families are: Apiaceaea and Rosaceae (4 plants). The aerial parts, Young silks, seed, fruits, roots, buds and leaves of plants were used by people. The plants were collected in desire months, dried and stored for use during the year seasons in order to use them later.
Conclusion: This study showed that, Heracleum persicum, Cichorium intybus, Mentha aquatica, Ziziphora clinopodioides and Mespilus germanica, are the plants used in high range by many people for their illness. Since these species have been used by ages in traditional therapy of the area, more attention should be paid for their scientific study.
Background: Iran has a long history of traditional medicine and medicinal plants use in Middle East. Many ethnics in several parts of Iran use medicinal plants for their illness.
Objective: Our aims were to identify which medicinal plants in the East- Mazandaran used for gastro intestinal problems and how the indigenous people treat their gastro intestinal problems.
Methods: An investigation was carried out for a period of 2 years (from 2014 to 2015) in the area of East- Mazandaran. A questionnaire was prepared through face-to-face interviews. In order to collect detailed information we visited the people who had knowledge of medicinal plants and identify their collected dried medicinal plants. Persian and local name of the plants, their utilized parts and preparation methods were investigated and recorded.
Results: After ethnobotanical study in the East-Mazandaran region we recorded 20 species belong to 10 family of medicinal plant in this area. The most common families are: Apiaceaea and Rosaceae (4 plants). The aerial parts, Young silks, seed, fruits, roots, buds and leaves of plants were used by people. The plants were collected in desire months, dried and stored for use during the year seasons in order to use them later.
Conclusion: This study showed that, Heracleum persicum, Cichorium intybus, Mentha aquatica, Ziziphora clinopodioides and Mespilus germanica, are the plants used in high range by many people for their illness. Since these species have been used by ages in traditional therapy of the area, more attention should be paid for their scientific study.
Ethnobotany, East- Mazandaran, Gastro intestinal, Iran, Medicinal plants, Traditional remedy
Ethnobotany, East- Mazandaran, Gastro intestinal, Iran, Medicinal plants, Traditional remedy
43
56
http://jmp.ir/browse.php?a_code=A-10-87-4&slc_lang=en&sid=1
2016/06/292017/02/262016/02/22
1394/12/3
2016/12/42017/07/252017/04/12
1396/1/23
Maryam
Ahvazi
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
مریم
اهوازی
Maryame_ahvazi@yahoo.com
00319475328460041309
00319475328460041309
Yes
مرکز تحقیقات گیاهان دارویی،پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج ایران
Mohammad
Akbarzadeh
Research Institute of Forest and Rangeland, Sari, Iran
محمد
اکبرزاده
00319475328460041310
00319475328460041310
No
مرکز تحقیقات کشاورزی ومنابع طبیعی مازندران، ساری
en
Ficus auriculata (fig) Extracts Induced Cell Cycle Profile Changes and Apoptosis Through Caspase-Independent Pathway in Human Lung Adenocarcinoma Cell Line, A549
Ficus auriculata (fig) Extracts Induced Cell Cycle Profile Changes and Apoptosis Through Caspase-Independent Pathway in Human Lung Adenocarcinoma Cell Line, A549
Background: Ficus auriculata (fig) has immense value of benefits with regards to their medicinal and therapeutic properties. It has been long used in traditional folk medicine, and one of the fruits mentioned in Al-Quran. Many scientific researches have proven the usage of this natural medicine in in vitro and in vivo studies, where anti-cancer is among of its recognized properties.
Objective: To acquire further details on how F. auriculata (fig) inhibited cancer growth, we investigated the effects of its exposure on cell cycle profile.
Methods: Subsequently we determined the type of cell death and the mechanism it induced using flow cytometer. Human lung adenocarcinoma cell line A549 was selected as the model of this study.
Results: It is indicated significant decreased of A549 cells in G0/G1and concurrent accumulation of cells in G2/M phase. Cell death analysis revealed significant late apoptosis induced by F. auriculata (fig) via caspase independent pathway.
Conclusion: Therefore, we concluded that F. auriculata (fig) is one of the promising natural medicines that can be used as cancer preventive strategy, as a result from its effects against cell cycle profile and apoptosis via caspase independent pathway.
Background: Ficus auriculata (fig) has immense value of benefits with regards to their medicinal and therapeutic properties. It has been long used in traditional folk medicine, and one of the fruits mentioned in Al-Quran. Many scientific researches have proven the usage of this natural medicine in in vitro and in vivo studies, where anti-cancer is among of its recognized properties.
Objective: To acquire further details on how F. auriculata (fig) inhibited cancer growth, we investigated the effects of its exposure on cell cycle profile.
Methods: Subsequently we determined the type of cell death and the mechanism it induced using flow cytometer. Human lung adenocarcinoma cell line A549 was selected as the model of this study.
Results: It is indicated significant decreased of A549 cells in G0/G1and concurrent accumulation of cells in G2/M phase. Cell death analysis revealed significant late apoptosis induced by F. auriculata (fig) via caspase independent pathway.
Conclusion: Therefore, we concluded that F. auriculata (fig) is one of the promising natural medicines that can be used as cancer preventive strategy, as a result from its effects against cell cycle profile and apoptosis via caspase independent pathway.
Anti-cancer, Apoptosis, Natural medicine, Lung cancer
Anti-cancer, Apoptosis, Natural medicine, Lung cancer
57
67
http://jmp.ir/browse.php?a_code=A-10-1491-3&slc_lang=en&sid=1
2016/06/292017/02/262016/02/222017/03/9
1395/12/19
2016/12/42017/07/252017/04/122017/06/17
1396/3/27
EF
Jamil
Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan Pahang, Malaysia
الینا فتینی
جمیل
00319475328460035490
00319475328460035490
No
Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan Pahang, Malaysia
RA
Ghani
Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan Pahang, Malaysia
ردیحه عبدل
قانی
00319475328460035491
00319475328460035491
Yes
Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan Pahang, Malaysia
en
Clinical Efficacy of Topical Avena sativa Versus Betamethasone in Chronic Pruritus due to Sulfur Mustard Exposure
Clinical efficacy of topical Avena sativa versus Betamethasone in chronic pruritus due to sulfur mustard exposure
Background: Avena sativa, a well-known herbal medicine; has been used in various skin diseases such as eczema, burn and pruritus.
Objective: The objective of this study was to evaluate the effect of this herbal medicine for treatment of chronic pruritus in Sulfur Mustard (SM) exposed patients.
Methods: Veterans who referred to Baghiat-Allah dermatologic clinic for itching problems were examined by a dermatologist and randomly assigned in three different groups. Group A received ointment derived from Avena sativa plant, group B, placebo and group C, betamethasone 0.1% cream twice a day for 4 weeks. Twenty five patients were included in each group. A visual analogue scale were used for assessment of severity of pruritus and 2 questionnaire for quality of life and quality of sleep were filled for each patient.
Results: Pruritus severity after the study by VAS method was significantly decreased in all the groups, but betamethasone group showed the largest decrease (-2.4, P<0.001). The average quality of life based on DLQI criteria and quality of sleep based on PSQI after the treatment showed the most significant difference in betamethasone group (3.52, P<0.001 and 0.96, P=0.001 respectively). Although Avena sativa showed significant effect on these criteria but it was only superior to placebo and not as effective as betamethasone.
Conclusion: The result demonstrated that Avena sativa ointment reduced chronic pruritus, increased quality of life and quality of sleep in patients exposed to SM but betamethasone was superior in all aspects.
Background: Avena sativa, a well-known herbal medicine; has been used in various skin diseases such as eczema, burn and pruritus.
Objective: The objective of this study was to evaluate the effect of this herbal medicine for treatment of chronic pruritus in Sulfur Mustard (SM) exposed patients.
Methods: Veterans who referred to Baghiat-Allah dermatologic clinic for itching problems were examined by a dermatologist and randomly assigned in three different groups. Group A received ointment derived from Avena sativa plant, group B, placebo and group C, betamethasone 0.1% cream twice a day for 4 weeks. Twenty five patients were included in each group. A visual analogue scale were used for assessment of severity of pruritus and 2 questionnaire for quality of life and quality of sleep were filled for each patient.
Results: Pruritus severity after the study by VAS method was significantly decreased in all the groups, but betamethasone group showed the largest decrease (-2.4, P<0.001). The average quality of life based on DLQI criteria and quality of sleep based on PSQI after the treatment showed the most significant difference in betamethasone group (3.52, P<0.001 and 0.96, P=0.001 respectively). Although Avena sativa showed significant effect on these criteria but it was only superior to placebo and not as effective as betamethasone.
Conclusion: The result demonstrated that Avena sativa ointment reduced chronic pruritus, increased quality of life and quality of sleep in patients exposed to SM but betamethasone was superior in all aspects.
Avena sativa, Chronic pruritus, Sulfur mustard
,Avena sativa, Chronic pruritus, Sulfur mustard
68
77
http://jmp.ir/browse.php?a_code=A-10-1678-2&slc_lang=en&sid=1
2016/06/292017/02/262016/02/222017/03/92016/04/20
1395/2/1
2016/12/42017/07/252017/04/122017/06/172017/08/12
1396/5/21
M
Shohrati
Research Center of Chemical Injuries, Baqiyatallah University of Medical Sciences, Tehran, Iran
مجید
شهرتی
shohratimajid@yahoo.com
00319475328460041311
00319475328460041311
Yes
مرکز تحقیقات آسیب های شیمیایی، دانشگاه علوم پزشکی بقیه اله (عج)، تهران، ایران
M
Davoudi
Department of Dermatology, Baqiyatallah University of Medical Sciences, Tehran, Iran
سید مسعود
داودی
davoudi_sm@yahoo.com
00319475328460041312
00319475328460041312
No
گروه درماتولوژی، دانشگاه علوم پزشکی بقیه اله (عج)، تهران، ایران
Sh
Rezazadeh
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
شمسعلی
رضازاده
shrezazadeh@yahoo.com
00319475328460041313
00319475328460041313
No
گروه پژوهشی فارماکوگنوزی و داروسازی مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاددانشگاهی، کرج، ایران
B
Najafian
Department of Pediatrics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
بیتا
نجفیان
bitanajafian@yahoo.com
00319475328460041314
00319475328460041314
No
بخش اطفال، دانشکده پزشکی، دانشگاه علوم پزشکی بقیه اله (عج)، تهران، ایران
en
Effect of Pistacia atlantica Mastic Extract on Experimental Wound Healing and Various Biochemical Parameters of Blood Serum in Rabbit Models
Effect of Pistacia atlantica Mastic Extract on Experimental Wound Healing and Various Biochemical Parameters of Blood Serum in Rabbit Models
Background: The mastic extract of Pistacia species can be considered as a natural herbal source in the pharmaceutical industry.
Objective: The purpose of this study was to evaluate the effectiveness of Pistacia atlantica mastic on wound healing, oxidative and blood biochemical parameters.
Methods: Topical cream and oral suspension was made of the mastic of P. atlantica, after preparing hydroalcoholic extract and essence. 40 male rabbits were selected (4 experimental groups and 4 control group). To induce oxidative stress and tissue damage the burn wound were created. Topical cream (1 gram 60% and 30%, 3 ml oral suspension 60% and 30%, respectively were used daily in treatment groups for 21 days. Inert cream and distilled water were used topically and orally in the control group, respectively. The oxidative parameters of blood serum including malondialdehyde (MDA), catalase, glutathione peroxidase, superoxide dismutase and biochemical parameters of low density and high lipoproteins, as well as glucose level were measured on day 1, 7, 14 and 21. The healing process and grading were evaluated by histological examination on day 21.
Results: Increased concentration of MDA indicated the appearance of oxidative stress. oncentrations of SOD, CAT, GPX showed significant increases as compared with the control group and in day 1 (P≤0.05). The level of LDL did not show any difference in experimental group.
Conclusion: The results show that mastic extract of P. atlantica has an appropriate effect on wound healing and can be used as a natural antioxidant.
Background: The mastic extract of Pistacia species can be considered as a natural herbal source in the pharmaceutical industry.
Objective: The purpose of this study was to evaluate the effectiveness of Pistacia atlantica mastic on wound healing, oxidative and blood biochemical parameters.
Methods: Topical cream and oral suspension was made of the mastic of P. atlantica, after preparing hydroalcoholic extract and essence. 40 male rabbits were selected (4 experimental groups and 4 control group). To induce oxidative stress and tissue damage the burn wound were created. Topical cream (1 gram 60% and 30%, 3 ml oral suspension 60% and 30%, respectively were used daily in treatment groups for 21 days. Inert cream and distilled water were used topically and orally in the control group, respectively. The oxidative parameters of blood serum including malondialdehyde (MDA), catalase, glutathione peroxidase, superoxide dismutase and biochemical parameters of low density and high lipoproteins, as well as glucose level were measured on day 1, 7, 14 and 21. The healing process and grading were evaluated by histological examination on day 21.
Results: Increased concentration of MDA indicated the appearance of oxidative stress. oncentrations of SOD, CAT, GPX showed significant increases as compared with the control group and in day 1 (P≤0.05). The level of LDL did not show any difference in experimental group.
Conclusion: The results show that mastic extract of P. atlantica has an appropriate effect on wound healing and can be used as a natural antioxidant.
Pistacia atlantica, Antioxidant, Blood parameters, Hydroalcoholic extract, Wound-healing
,Pistacia atlantica, Antioxidant, Blood parameters, Hydroalcoholic extract, Wound-healing
78
91
http://jmp.ir/browse.php?a_code=A-10-1480-2&slc_lang=en&sid=1
2016/06/292017/02/262016/02/222017/03/92016/04/202017/03/1
1395/12/11
2016/12/42017/07/252017/04/122017/06/172017/08/122017/07/30
1396/5/8
Sh
Fakour
Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
شاهین
فکور
00319475328460041315
00319475328460041315
Yes
Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
S
Heydari
Graduated of Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
سامان
حیدری
00319475328460041316
00319475328460041316
No
Graduated of Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
L
Akradi
Department of Pathobiology, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
لقمان
اکبری
00319475328460041317
00319475328460041317
No
Department of Pathobiology, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
R
Rahymi Bane
Department of Agronomy and Breeding Plant, Faculty of Agriculture and Natural Resource, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
رحمان
رحیامی بانه
00319475328460041318
00319475328460041318
No
Department of Agronomy and Breeding Plant, Faculty of Agriculture and Natural Resource, Sanandaj Branch Islamic Azad University, Sanandaj, Iran
en
Phytochemical Evaluation and Plant Regeneration Through Somatic Embryogenesis of Ferulago angulata subsp. Carduchorum (Boiss. and Hausskn), an Endangered Medicinal Plant
Phytochemical Evaluation and Plant Regeneration Through Somatic Embryogenesis of Ferulago angulata subsp. Carduchorum (Boiss. and Hausskn), an Endangered Medicinal Plant
Background: Ferulago angulata is an important medicinal plant and endemic to Iran which is an endangered Plant.
Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat.
Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was analyzed by the GC and GC-MS. For callus induction, explants were cultured on MS medium supplemented with NAA, 2,4-D and BA. For somatic embryogenesis, the calli were cultured on MS medium supplemented with full, 1/4 and 1/10 fold of growth regulators and MS medium without growth regulator. For maturation, embryogenic clumps were transferred on MS medium supplemented with BA, 2,4-D and 30 or 60 g l-1 sucrose. The small embryogenic clumps were transferred to medium with GA3.
Results: The main components of essential oils were Ocimene (29.84%), followed by α-pinene (27.01%), Isobornyl formate (7.7%) and gamma-Terpinene (4.85%). The highest percentage of callus induction observed in the root and hypocotyl explants. The maximum somatic embryogenesis was obtained in MS medium with 0.5 mg l-1 2, 4-D via hypocotyls derived explants. The highest frequency of maturation was obtained in MS medium containing 30 g l-1 sucrose without growth regulators.
Conclution: This plant had a noticeable amount of essential oil with valuable ingredients and tissue culture techniques could be applied for its mass propagation, conservation of germplasm and in vitro regeneration.
Background: Ferulago angulata is an important medicinal plant and endemic to Iran which is an endangered Plant.
Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat.
Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was analyzed by the GC and GC-MS. For callus induction, explants were cultured on MS medium supplemented with NAA, 2,4-D and BA. For somatic embryogenesis, the calli were cultured on MS medium supplemented with full, 1/4 and 1/10 fold of growth regulators and MS medium without growth regulator. For maturation, embryogenic clumps were transferred on MS medium supplemented with BA, 2,4-D and 30 or 60 g l-1 sucrose. The small embryogenic clumps were transferred to medium with GA3.
Results: The main components of essential oils were Ocimene (29.84%), followed by α-pinene (27.01%), Isobornyl formate (7.7%) and gamma-Terpinene (4.85%). The highest percentage of callus induction observed in the root and hypocotyl explants. The maximum somatic embryogenesis was obtained in MS medium with 0.5 mg l-1 2, 4-D via hypocotyls derived explants. The highest frequency of maturation was obtained in MS medium containing 30 g l-1 sucrose without growth regulators.
Conclution: This plant had a noticeable amount of essential oil with valuable ingredients and tissue culture techniques could be applied for its mass propagation, conservation of germplasm and in vitro regeneration.
Ferulago angulata, Callus induction, Embryogenesis, Essential oil
,Ferulago angulata, Callus induction, Embryogenesis, Essential oil
92
107
http://jmp.ir/browse.php?a_code=A-10-75-7&slc_lang=en&sid=1
2016/06/292017/02/262016/02/222017/03/92016/04/202017/03/12017/02/9
1395/11/21
2016/12/42017/07/252017/04/122017/06/172017/08/122017/07/302017/07/5
1396/4/14
HA
Naghdi Badi
Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran
حسنعلی
نقدی بادی
Naghdibadi@yahoo.com
00319475328460041319
00319475328460041319
Yes
مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران
Sh
Salek Hashemi
Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran
شهناز
سالک هاشمی
salekshahnaz@yahoo.com
00319475328460041320
00319475328460041320
No
دانشجوی کارشناسی ارشد، گروه بیوتکنولوژی و اصلاح نباتات ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ، ایران
AA
Nasrollahnezhad Qomi
Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran
علیاصغر
نصرالله نژاد قمی
Ali1346nn@yahoo.com
00319475328460041321
00319475328460041321
No
عضو هیئت علمی گروه بیوتکنولوژی و اصلاح نباتات ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ، ایران
Saeed
Navabpour
Department of Plant Breeding and Biotechnology, University of agricultural science and natural resources Gorgan , Gorgan,Iran
سعید
نوابپور
S.navabpour@yahoo.com
00319475328460041322
00319475328460041322
No
عضو هیئت علمی گروه بیوتکنولوژی و اصلاح نباتات ، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ، ایران
A
Qaderi
Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran
اردشیر
قادری
Ardeshir582008@gmail.com
00319475328460041323
00319475328460041323
No
مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران
en
Pergularia tomentosa, from Traditional Uses to Ecology and Phytochemistry
Pergularia tomentosa, from Traditional Uses to Ecology and Phytochemistry
Background: Pergularia tomentosa L. has several traditional uses and biological activities which need to be more investigated.
Objective: In this study, some ecological and phytochemical properties of Pergularia tomentosa were assessed. The flavonoids and cardenolides in the aqueous extract of leaves were identified with LCMS techniques.
Methods: Some quantity characteristics of P. tomentosa were measured. Extraction was performed according to the traditional method (aqueous extract at room temperature). Metabolite profiling was conducted using untargeted liquid chromatography coupled to high resolution mass spectrometry (LCeESI-OrbitrapeMS). Data were analyzed by Xcalibur version 2.1.
Results: Results showed that length of roots, leaves and fruits and the height of aerial parts were 23-30 cm, 2.5-3.2 cm, 4.9- 5.4 cm and 40-58.00 cm, respectively. Physiography and canopy cover were two important variables on these properties. We also identified 19 natural products in aqueous extract, 15 phenolics and flavonoids and 4 cardenolides. Three flavonoids (8, 11 and 14) and one cardenolide (17) had been previously isolated in P. tomentosa and 3 cardenolides (5, 18 and 19) has been reported for the first time in this plant.
Conclusion: Results indicated that the leaves of P. tomentosa are a rich source of flavonoids and cardenolides. These findings suggest that leaves of this plant can be a good source of natural antioxidant and antitumor compounds. However, this plant needs special conservational efforts. Therefore, planting this plant under the canopy or along the borders of waterways can help in an appropriate habitat for its conservation and reclamation.
Background: Pergularia tomentosa L. has several traditional uses and biological activities which need to be more investigated.
Objective: In this study, some ecological and phytochemical properties of Pergularia tomentosa were assessed. The flavonoids and cardenolides in the aqueous extract of leaves were identified with LCMS techniques.
Methods: Some quantity characteristics of P. tomentosa were measured. Extraction was performed according to the traditional method (aqueous extract at room temperature). Metabolite profiling was conducted using untargeted liquid chromatography coupled to high resolution mass spectrometry (LCeESI-OrbitrapeMS). Data were analyzed by Xcalibur version 2.1.
Results: Results showed that length of roots, leaves and fruits and the height of aerial parts were 23-30 cm, 2.5-3.2 cm, 4.9- 5.4 cm and 40-58.00 cm, respectively. Physiography and canopy cover were two important variables on these properties. We also identified 19 natural products in aqueous extract, 15 phenolics and flavonoids and 4 cardenolides. Three flavonoids (8, 11 and 14) and one cardenolide (17) had been previously isolated in P. tomentosa and 3 cardenolides (5, 18 and 19) has been reported for the first time in this plant.
Conclusion: Results indicated that the leaves of P. tomentosa are a rich source of flavonoids and cardenolides. These findings suggest that leaves of this plant can be a good source of natural antioxidant and antitumor compounds. However, this plant needs special conservational efforts. Therefore, planting this plant under the canopy or along the borders of waterways can help in an appropriate habitat for its conservation and reclamation.
P. tomentosa, Cardenolide, Flavonoid, LC-ESI-Orbitrap-MS/MS, Quantity characteristics
,P. tomentosa, Cardenolide, Flavonoid, LC-ESI-Orbitrap-MS/MS, Quantity characteristics
108
118
http://jmp.ir/browse.php?a_code=A-10-1536-1&slc_lang=en&sid=1
2016/06/292017/02/262016/02/222017/03/92016/04/202017/03/12017/02/92017/04/24
1396/2/4
2016/12/42017/07/252017/04/122017/06/172017/08/122017/07/302017/07/52017/06/17
1396/3/27
SH
Hosseini Kahnouj
Department of Reclamation of Arid Mountainous Regions, Natural Resources Faculty, University of Tehra
سیدحمزه
حسینی کهنوج
shhosseinik@ut.ac.ir
00319475328460041324
00319475328460041324
No
Department of Reclamation of Arid and Mountainous Regions, Natural Resources Faculty, University of Tehran, Iran
M
Ayyari
Department of Horticultural Science, Tarbiat Modares University (TMU), Tehran, Iran
مهدی
عیاری
m.ayyari@modares.ac.ir
00319475328460041325
00319475328460041325
No
Department of Horticultural Science, Tarbiat Modares University (TMU), Tehran, Iran
Hossein
Azarnivand
Department of Reclamation of Arid Mountainous Regions, Natural Resources Faculty, University of Tehran, Iran
حسین
آذرنیوند
hazar@ut.ac.ir
00319475328460041326
00319475328460041326
Yes
Department of Reclamation of Arid Mountainous Regions, Natural Resources Faculty, University of Tehran, Iran
S
Piacente
4Department of Farmacy, University of Salerno, Italy
سونیا
پیاسنت
piacenteae@unisa.it
00319475328460041327
00319475328460041327
No
Department of Pharmacy, University of Salerno, Italy
MA
Zare Chahouki
Department of Reclamation of Arid Mountainous Regions, Natural Resources Faculty, University of Tehran, Iran
محمدعلی
زارع چاهوکی
mazar@ut.aciir
00319475328460041328
00319475328460041328
No
Department of Reclamation of Arid Mountainous Regions, Natural Resources Faculty, University of Tehran, Iran
en
Diversity Evaluation of Trigonella foenum-graecum Populations Using DNA Markers and Phytochemical Characteristics
Diversity Evaluation of Trigonella foenum-graecum Populations Using DNA Markers and Phytochemical Characteristics
Background: Fenugreek (Trigonella foenum-graecum L.) is an important medicinal plant that is a source of diosgenin, trigonelline and other valuable components. Fenugreek seeds is used for its pharmaceutical and nutraceutical properties.
Objective: The aim of present study was to investigate the genetic diversity within and between different populations of Trigonella foenum-graecum using DNA markers and phytochemical characteristics.
Methods: The total genomic DNA was isolated from 12 populations of Trigonella foenum-graecum collected from different regions in Iran. After optimizing PCR, 10 ISSR and 10 SCoT primers were used to amplify the genomic DNA of all genotypes. To investigate genetic distance, cluster analysis based on Jaccard similarity coefficients and UPGMA algorithm calculated for the 12 genotypes. Also, the trigonelline content was determined between 2 populations which had the highest genetic distance.
Results: Analysis of banding patterns of 10 SCoT primers revealed 92 polymorphic bands with an average of 9.2 fragments per prime. A total of 79 bands were generated from 10 ISSR primers, out of which 55 (68.21%) were polymorphic bands. The average values of dissimilarities based on the SCoT and ISSR data were 0.60 and 0.44, respectively. Also, shoot trigonelline content was significantly different between genotypes with the highest genetic distance.
Conclusion: Our results revealed a high level of genetic variation among fenugreek populations. The findings also indicated that SCoT technique is more informative than ISSR technique for evaluation of genetic diversity and relationships among fenugreek populations. The genotypes with the highest genetic distance based on SCoT data, were noticeably different in respect of trigonelline content.
Background: Fenugreek (Trigonella foenum-graecum L.) is an important medicinal plant that is a source of diosgenin, trigonelline and other valuable components. Fenugreek seeds is used for its pharmaceutical and nutraceutical properties.
Objective: The aim of present study was to investigate the genetic diversity within and between different populations of Trigonella foenum-graecum using DNA markers and phytochemical characteristics.
Methods: The total genomic DNA was isolated from 12 populations of Trigonella foenum-graecum collected from different regions in Iran. After optimizing PCR, 10 ISSR and 10 SCoT primers were used to amplify the genomic DNA of all genotypes. To investigate genetic distance, cluster analysis based on Jaccard similarity coefficients and UPGMA algorithm calculated for the 12 genotypes. Also, the trigonelline content was determined between 2 populations which had the highest genetic distance.
Results: Analysis of banding patterns of 10 SCoT primers revealed 92 polymorphic bands with an average of 9.2 fragments per prime. A total of 79 bands were generated from 10 ISSR primers, out of which 55 (68.21%) were polymorphic bands. The average values of dissimilarities based on the SCoT and ISSR data were 0.60 and 0.44, respectively. Also, shoot trigonelline content was significantly different between genotypes with the highest genetic distance.
Conclusion: Our results revealed a high level of genetic variation among fenugreek populations. The findings also indicated that SCoT technique is more informative than ISSR technique for evaluation of genetic diversity and relationships among fenugreek populations. The genotypes with the highest genetic distance based on SCoT data, were noticeably different in respect of trigonelline content.
Trigonella foenum-graecum L., Genetic variation, ISSR, SCoT
,Trigonella foenum-graecum L., Genetic variation, ISSR, SCoT
119
132
http://jmp.ir/browse.php?a_code=A-10-472-5&slc_lang=en&sid=1
2016/06/292017/02/262016/02/222017/03/92016/04/202017/03/12017/02/92017/04/242017/05/7
1396/2/17
2016/12/42017/07/252017/04/122017/06/172017/08/122017/07/302017/07/52017/06/172017/06/14
1396/3/24
H
Daneshmand
Department of Biotechnology and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
حدیث
دانشمند
00319475328460041329
00319475328460041329
Yes
Department of Biotechnology and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
AR
Etminan
Department of Biotechnology and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
علیرضا
اطمینان
00319475328460041330
00319475328460041330
No
Department of Biotechnology and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
A
Qaderi
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
اردشیر
قادری
00319475328460041331
00319475328460041331
No
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran