en
jalali
1392
6
1
gregorian
2013
9
1
12
47
online
1
fulltext
en
Saffron a Prototype Example for Evidence Based Herbal Medicine
Saffron a Prototype Example for Evidence Based Herbal Medicine
Evidence-based medicine is now generally perceived to be the dominant operating system in conventional medicine. Evidence-based medicine developed concurrently with the internet and the world wide web. This is no coincidence since evidence-based medicine suggests a personal responsibility for clinicians to keep abreast of research that would be difficult without the information access that the web provides. Over the last two decades, evidence based herbal medicine has been considered as interesting field. One of prototype example of evidence base medicine is saffron. In this paper, we review saffron from evidence point of view.
Evidence-based medicine is now generally perceived to be the dominant operating system in conventional medicine. Evidence-based medicine developed concurrently with the internet and the world wide web. This is no coincidence since evidence-based medicine suggests a personal responsibility for clinicians to keep abreast of research that would be difficult without the information access that the web provides. Over the last two decades, evidence based herbal medicine has been considered as interesting field. One of prototype example of evidence base medicine is saffron. In this paper, we review saffron from evidence point of view.
Alzheimer’s disease, Depression, Evidence-based medicine, Saffron
Alzheimer’s disease, Depression, Evidence-based medicine, Saffron
1
7
http://jmp.ir/browse.php?a_code=A-10-34-3&slc_lang=en&sid=1
2013/07/21
1392/4/30
2013/10/5
1392/7/13
R
Hajiaghaee
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
R
Hajiaghaee
00319475328460036623
00319475328460036623
No
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
M
Heidari
Iranian Academic Center for Education, Culture & Research (ACECR)
M
Heidari
00319475328460036624
00319475328460036624
No
Iranian Academic Center for Education, Culture & Research (ACECR)
S
Akhondzadeh
Psychiatric Research Center, Roozbeh Hospital, Tehran University of Medical Sciences
S
Akhondzadeh
s.akhond@neda.net
00319475328460036625
00319475328460036625
Yes
Psychiatric Research Center, Roozbeh Hospital, Tehran University of Medical Sciences
en
A Review on Phytochemistry and Pharmacology of Otostegia persica (Burm.f.) Boiss.
A Review on Phytochemistry and Pharmacology of Otostegia persica (Burm.f.) Boiss.
Iran has a great wealth of various naturally occurring medicinal plants which have great potential pharmacological activities. Otostegia persica (Burm.f.) Boiss. is one of these plants which is a perennial shrub found in South and Southeast of Iran. The aerial parts of O. persica have been used in Iranian traditional medicine to treat various types of diseases (e.g., inflammatoryand pain-mediated diseases, malaria and diabetes). Research carried out using different in vivo and in vitro assays of biological evaluation support most of these claims. The active pharmacological components of O. persica most studied are flavonoids and terpenoids. This paper presents the traditional uses, botany, phytochemistry, and pharmacology of this medicinal plant. This is the first article reported in this review form about O. persica which might be helpful for scientists and researchers to find out new in modern health concept.
Iran has a great wealth of various naturally occurring medicinal plants which have great potential pharmacological activities. Otostegia persica (Burm.f.) Boiss. is one of these plants which is a perennial shrub found in South and Southeast of Iran. The aerial parts of O. persica have been used in Iranian traditional medicine to treat various types of diseases (e.g., inflammatoryand pain-mediated diseases, malaria and diabetes). Research carried out using different in vivo and in vitro assays of biological evaluation support most of these claims. The active pharmacological components of O. persica most studied are flavonoids and terpenoids. This paper presents the traditional uses, botany, phytochemistry, and pharmacology of this medicinal plant. This is the first article reported in this review form about O. persica which might be helpful for scientists and researchers to find out new in modern health concept.
Otostegia persica, Lamiaceae, Pharmacology, Phytochemistry
Otostegia persica, Lamiaceae, Pharmacology, Phytochemistry
8
18
http://jmp.ir/browse.php?a_code=A-10-89-1&slc_lang=en&sid=1
2013/07/212013/07/29
1392/5/7
2013/10/52013/09/29
1392/7/7
J
Asgarpanah
Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU)
J
Asgarpanah
asgarpanah@iaups.ac.ir
00319475328460042039
00319475328460042039
Yes
Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU)
S
Mohammadi Motamed
Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU)
S
Mohammadi Motamed
00319475328460042040
00319475328460042040
No
Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University (IAU)
en
A Review on Pharmacological, Cultivation and Biotechnology Aspects of Milk Thistle (Silybum marianum (L.) Gaertn.)
A Review on Pharmacological, Cultivation and Biotechnology Aspects of Milk Thistle (Silybum marianum (L.) Gaertn.)
Milk thistle (Silybum marianum (L.) Gaertn.) is one of the valuable medicinal plants which used in the treatment of liver disorders. The major active constituents in this plant are flavonolignans, collectively known as silymarin which is a mixture of three isomer silybin, silydianin and silycristin. Its therapeutic properties are due to the presence of silymarin. The seeds contain the highest amount of silymarin, but the other plant parts have less amount of this compound. The silymarin content in fruits depends on milk thistle variety and geographic and climatic condition. In this review, we summarized the accomplished investigations on aspects of medicinal, cultivation, biology and biotechnology of milk thistle.
Milk thistle (Silybum marianum (L.) Gaertn.) is one of the valuable medicinal plants which used in the treatment of liver disorders. The major active constituents in this plant are flavonolignans, collectively known as silymarin which is a mixture of three isomer silybin, silydianin and silycristin. Its therapeutic properties are due to the presence of silymarin. The seeds contain the highest amount of silymarin, but the other plant parts have less amount of this compound. The silymarin content in fruits depends on milk thistle variety and geographic and climatic condition. In this review, we summarized the accomplished investigations on aspects of medicinal, cultivation, biology and biotechnology of milk thistle.
Silybum marianum, Silymarin, Silybin, Flavonolignan, Cultivation and Medicinal properties
Silybum marianum, Silymarin, Silybin, Flavonolignan, Cultivation and Medicinal properties
19
37
http://jmp.ir/browse.php?a_code=A-10-59-3&slc_lang=en&sid=1
2013/07/212013/07/292013/05/4
1392/2/14
2013/10/52013/09/292013/07/9
1392/4/18
N
Qavami
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
N
Qavami
00319475328460042041
00319475328460042041
No
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
H
Naghdi Badi
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
H
Naghdi Badi
00319475328460042042
00319475328460042042
No
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
MR
Labbafi
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
MR
Labbafi
00319475328460042043
00319475328460042043
No
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
A
Mehrafarin
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
A
Mehrafarin
A.Mehrafarin@gmail.com
00319475328460042044
00319475328460042044
Yes
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
en
A Comparative Study of Hypolipidemic Activities of the Extracts of Melissa officinalis and Berberis vulgaris in Rats
A Comparative Study of Hypolipidemic Activities of the Extracts of Melissa officinalis and Berberis vulgaris in Rats
Background: Hyperlipidemia is a heterogeneous group of disorders characterized by an excess of lipids in the bloodstream Objective: Given previous studies on barberry and Melissa officinalis extracts, this study aims at comparing hypolipidemic activities of Melissa officinalis extract and Berberis vulgaris. Methods: For the purpose of this study, 64 Wistar rats were selected and divided into 8 groups (n=8). The control group was administered with ordinary diet the sham group was administered with high-fat diet and intraperitoneally 0.2 ml/dl of the extract solvent (normal saline) and similarly, experimental groups received minimal, moderate and maximum dosages of barberry and Melissa officinalis extracts. The treatment group's was given high-fat diet for 21 days. After this period, blood samples were taken and the gathered data were analyzed using SPSS software. Results: The amount of cholesterol, triglyceride and LDL were increased in the model group compared to the control group, whereas the same substances were decreased significantly in the group receiving the extract compared to the model group (p<0.05). Conclusion: Hypolipidemic properties of alcohol extracts of Melissa officinalis are more effective than those of Berberis vulgaris. Moreover, it should be noted that it is rather the antioxidant properties of Melissa officinalis and their effects on the increase in thyroid hormones as well as the presence of alkaloid compounds, such as berberine in Berberis vulgaris, that inhibits cholesterol synthesis and enables its excretion.
Background: Hyperlipidemia is a heterogeneous group of disorders characterized by an excess of lipids in the bloodstream Objective: Given previous studies on barberry and Melissa officinalis extracts, this study aims at comparing hypolipidemic activities of Melissa officinalis extract and Berberis vulgaris. Methods: For the purpose of this study, 64 Wistar rats were selected and divided into 8 groups (n=8). The control group was administered with ordinary diet the sham group was administered with high-fat diet and intraperitoneally 0.2 ml/dl of the extract solvent (normal saline) and similarly, experimental groups received minimal, moderate and maximum dosages of barberry and Melissa officinalis extracts. The treatment group's was given high-fat diet for 21 days. After this period, blood samples were taken and the gathered data were analyzed using SPSS software. Results: The amount of cholesterol, triglyceride and LDL were increased in the model group compared to the control group, whereas the same substances were decreased significantly in the group receiving the extract compared to the model group (p<0.05). Conclusion: Hypolipidemic properties of alcohol extracts of Melissa officinalis are more effective than those of Berberis vulgaris. Moreover, it should be noted that it is rather the antioxidant properties of Melissa officinalis and their effects on the increase in thyroid hormones as well as the presence of alkaloid compounds, such as berberine in Berberis vulgaris, that inhibits cholesterol synthesis and enables its excretion.
Berberis vulgaris, Melissa officinalis, Cholesterol, Hypolipidemic, Rat
Berberis vulgaris, Melissa officinalis, Cholesterol, Hypolipidemic, Rat
38
47
http://jmp.ir/browse.php?a_code=A-10-81-2&slc_lang=en&sid=1
2013/07/212013/07/292013/05/42013/04/27
1392/2/7
2013/10/52013/09/292013/07/92013/08/7
1392/5/16
S
Changizi Ashtiyani
Department of Physiology, Arak University of Medical Sciences
S
Changizi Ashtiyani
dr.ashtiyani@arakmu.ac.ir
00319475328460042045
00319475328460042045
Yes
Department of Physiology, Arak University of Medical Sciences
A
Zarei
Department of Biology, Abadeh Branch, Islamic Azad University
A
Zarei
00319475328460042046
00319475328460042046
No
Department of Biology, Abadeh Branch, Islamic Azad University
S
Taheri
Staff of Research Deputy, Arak University of Medical Sciences
S
Taheri
00319475328460042047
00319475328460042047
No
Staff of Research Deputy, Arak University of Medical Sciences
A
Rezaei
Department of Physiology, Islamic Azad University of Arsanjan
A
Rezaei
00319475328460042048
00319475328460042048
No
Department of Physiology, Islamic Azad University of Arsanjan
M
Golshan
Department of Shiraz University of Medical Sciences
M
Golshan
00319475328460042049
00319475328460042049
No
Department of Shiraz University of Medical Sciences
R
Ghafarzadegan
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
R
Ghafarzadegan
00319475328460042050
00319475328460042050
No
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
en
Phytochemical, Agronomical and Morphological Responses of Pot Marigold (Calendula officinalis L.) to Foliar Application of Bio-stimulators (Bioactive Amino Acid Compounds)
Phytochemical, Agronomical and Morphological Responses of Pot Marigold (Calendula officinalis L.) to Foliar Application of Bio-stimulators (Bioactive Amino Acid Compounds)
Background: Bio-stimulators as biological substances stimulate metabolism and metabolic processes, which can be increase plants yield and secondary metabolites content. Objective: The aim is to introduce bio-stimulators as bioactive amino acid compounds to increase yield and help to sustainable agriculture. Methods: This experiment was conducted at ACECR on the base of randomized complete blocks design with three replications in 2011. The treatments were commercial formulations of Aminolforte, Kadostim, Fosnutren, Humiforte (each of them 0.75 and 1.5 L.ha-1), chemical fertilizer NPK (70 kg.ha-1), and control treatment (no foliar application). Results: The treatments had significant effect on the studied parameters except SPAD value. Humiforte 1.5 L.ha-1 caused increase in plant dry weight to 37.46%, capitula dry weight to 36.92%, leaf area to 34.44%, relative water content to 32.03%, harvest index to 20.40%, capitula number/plant to 40.64%, total carbohydrates of capitula to 18.43%, total flavonoids of leaves to 19.35%, and total flavonoids yield of leaves to 38.63% compared to control. The highest amount of total flavonoids in capitula (0.25%, with increase to 32% compared to control) was related to Aminolforte 1.5 L.ha-1. Kadostim 1.5 L.ha-1 increased plant height to 36.83%, leaves number to 26.65% and total flavonoids yield of capitula to 38.82%, total carbohydrates of leaves to 13.52%. Content of N, P and K increased with foliar application of bioactive amino acid compounds. Conclusion: Humiforte and Kadostim 1.5 L.ha-1 were the best treatments in regard to existence of amino acid compounds and macro-nutrients of N, P and K in their formulations.
Background: Bio-stimulators as biological substances stimulate metabolism and metabolic processes, which can be increase plants yield and secondary metabolites content. Objective: The aim is to introduce bio-stimulators as bioactive amino acid compounds to increase yield and help to sustainable agriculture. Methods: This experiment was conducted at ACECR on the base of randomized complete blocks design with three replications in 2011. The treatments were commercial formulations of Aminolforte, Kadostim, Fosnutren, Humiforte (each of them 0.75 and 1.5 L.ha-1), chemical fertilizer NPK (70 kg.ha-1), and control treatment (no foliar application). Results: The treatments had significant effect on the studied parameters except SPAD value. Humiforte 1.5 L.ha-1 caused increase in plant dry weight to 37.46%, capitula dry weight to 36.92%, leaf area to 34.44%, relative water content to 32.03%, harvest index to 20.40%, capitula number/plant to 40.64%, total carbohydrates of capitula to 18.43%, total flavonoids of leaves to 19.35%, and total flavonoids yield of leaves to 38.63% compared to control. The highest amount of total flavonoids in capitula (0.25%, with increase to 32% compared to control) was related to Aminolforte 1.5 L.ha-1. Kadostim 1.5 L.ha-1 increased plant height to 36.83%, leaves number to 26.65% and total flavonoids yield of capitula to 38.82%, total carbohydrates of leaves to 13.52%. Content of N, P and K increased with foliar application of bioactive amino acid compounds. Conclusion: Humiforte and Kadostim 1.5 L.ha-1 were the best treatments in regard to existence of amino acid compounds and macro-nutrients of N, P and K in their formulations.
Calendula officinalis L., Bioactive amino acid compounds, Morphological and Phytochemical traits
Calendula officinalis L., Bioactive amino acid compounds, Morphological and Phytochemical traits
48
61
http://jmp.ir/browse.php?a_code=A-10-44-19&slc_lang=en&sid=1
2013/07/212013/07/292013/05/42013/04/272013/06/1
1392/3/11
2013/10/52013/09/292013/07/92013/08/72013/08/7
1392/5/16
H
Rafiee
Department of Horticulture, Science and Research branch, Islamic Azad University
H
Rafiee
00319475328460042051
00319475328460042051
No
Department of Horticulture, Science and Research branch, Islamic Azad University
A
Mehrafarin
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
A
Mehrafarin
00319475328460042052
00319475328460042052
No
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
A
Qaderi
Biotechnology Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
A
Qaderi
00319475328460042053
00319475328460042053
No
Biotechnology Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
S
Kalate Jari
Department of Horticulture, Science and Research branch, Islamic Azad University
S
Kalate Jari
00319475328460042054
00319475328460042054
No
Department of Horticulture, Science and Research branch, Islamic Azad University
H
Naghdi Badi
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
H
Naghdi Badi
Naghdibadi@yahoo.com
00319475328460042055
00319475328460042055
Yes
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
en
Protective Effects of Vaccinium arctostaphylos L., Berberis thunbergii var. atropurpurea Chenault, Elaeagnus angustifolia L. and Launaea acanthodes (Boiss.) O. Kuntze against Indomethacin-Induced Gastric Ulcers in Rats
Protective Effects of Vaccinium arctostaphylos L., Berberis thunbergii var. atropurpurea Chenault, Elaeagnus angustifolia L. and Launaea acanthodes (Boiss.) O. Kuntze against Indomethacin-Induced Gastric Ulcers in Rats
Background: Data suggest that V. arctostaphylos, B. thunbergii var. atropurpurea, E. angustifolia and L. acanthodes may prevent gastric ulcers induced by Non-steroidal anti-inflammatory drugs (NSAIDs). Objective: To explore the ulcer-protective potential of these plants in indomethacin-induced gastric ulcers in rats. Methods: Saline, hydro-alcoholic extract of each plant (100, 400, 800 mg/kg) and omeprazole (30 mg/kg) were gavaged to the groups of animals for 4 consecutive days. Gastric ulcers were induced by the onetime gavage of indomethacin (30 mg/kg, p.o.). On the fifth day, each group was pretreated with physiological saline as control, extract (100, 400 or 800 mg/kg, p.o.) or omeprazole (30 mg/kg, p.o.) 30 min before the indomethacin administration. The animals were killed 6 h after the indomethacin administration. The stomachs were removed, opened along the greater curvature and washed in physiological saline. A person unaware of the type of treatment received by the animals examined the stomachs under a 3-fold magnifier. The areas and lengths of hemorrhagic lesions induced by indomethacin were measured using a dial caliper and the sum of measurements for each animal was referred to as the ulcer index. Results: All extracts reduced the ulcer index significantly compared to the control group (p < 0.05). Conclusion: These plants prevent NSAID-induced gastric ulcers in rats. The efficacy and potency of the gastro-protective effect of L. acanthodes appears to be higher than the other 3 plants.
Background: Data suggest that V. arctostaphylos, B. thunbergii var. atropurpurea, E. angustifolia and L. acanthodes may prevent gastric ulcers induced by Non-steroidal anti-inflammatory drugs (NSAIDs). Objective: To explore the ulcer-protective potential of these plants in indomethacin-induced gastric ulcers in rats. Methods: Saline, hydro-alcoholic extract of each plant (100, 400, 800 mg/kg) and omeprazole (30 mg/kg) were gavaged to the groups of animals for 4 consecutive days. Gastric ulcers were induced by the onetime gavage of indomethacin (30 mg/kg, p.o.). On the fifth day, each group was pretreated with physiological saline as control, extract (100, 400 or 800 mg/kg, p.o.) or omeprazole (30 mg/kg, p.o.) 30 min before the indomethacin administration. The animals were killed 6 h after the indomethacin administration. The stomachs were removed, opened along the greater curvature and washed in physiological saline. A person unaware of the type of treatment received by the animals examined the stomachs under a 3-fold magnifier. The areas and lengths of hemorrhagic lesions induced by indomethacin were measured using a dial caliper and the sum of measurements for each animal was referred to as the ulcer index. Results: All extracts reduced the ulcer index significantly compared to the control group (p < 0.05). Conclusion: These plants prevent NSAID-induced gastric ulcers in rats. The efficacy and potency of the gastro-protective effect of L. acanthodes appears to be higher than the other 3 plants.
Anti-ulcer, Herbal, Medicine, Rat, Traditional
Anti-ulcer, Herbal, Medicine, Rat, Traditional
62
69
http://jmp.ir/browse.php?a_code=A-10-71-2&slc_lang=en&sid=1
2013/07/212013/07/292013/05/42013/04/272013/06/12013/06/12
1392/3/22
2013/10/52013/09/292013/07/92013/08/72013/08/72013/09/14
1392/6/23
H
Fallah Huseini
Pharmacology & Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
H
Fallah Huseini
00319475328460036648
00319475328460036648
No
Pharmacology & Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
S
Kianbakht
Pharmacology & Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
S
Kianbakht
skianbakht@yahoo.com
00319475328460036649
00319475328460036649
Yes
Pharmacology & Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
M
Hoormand
Pharmacology & Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
M
Hoormand
00319475328460036650
00319475328460036650
No
Pharmacology & Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
en
Effects of Exogenous Salicylic Acid Application on Growth, Metabolic Activities and Essential Oil Composition of Satureja khuzistanica Jamzad
Effects of Exogenous Salicylic Acid Application on Growth, Metabolic Activities and Essential Oil Composition of Satureja khuzistanica Jamzad
Background: Satureja khuzistanica Jamzad, with the common Persian name “marzeh khuzestani”, is an endemic medicinal plant, distributed in the southwestern areas of Iran. Salicylic acid (SA) is a signaling molecule and a hormone-like substance that plays an important role in the plant physiological processes. Objective: This study was conducted to determine the influence of foliar SA application (0, 50, 100, 200 and 400 mg.L-1) at two times including vegetative stage (VS) and both vegetative and reproductive stages (VS+RS) on growth parameters, enzymes activity including superoxide dismutase (SOD) and polyphenol oxidase (PPO), prptein content, essential oil percentage and composition of S. khuzistanica under field conditions. Methods: The essential oils were isolated from aerial flowering parts of the plants by hydro-distillation method and then subjected to GC and GC-MS analyses to determine the oil constituents. Results: Results showed that SA application at 100 and 200 mg.L-1 were the most effective treatments in growth characteristics, but the highest essential oil content and yield was obtained at 400 mg.L-1 SA treatment. In both spraying times, plants treated with 100 and 200 mg.L-1 SA concentration showed more PPO and SOD activity than control plants, respectively. Also, results showed that the 14 compositions were identified in essential oil of plants under all employed treatments. Carvacrol was the major component of oils, which is also showed more variability than that of other components. Conclusion: It was concluded that foliar spray of SA at low concentration once at vegetative and second time at reproductive stage might be employed for enhancing both primary and secondary metabolites production of S. khuzistanica plants.
Background: Satureja khuzistanica Jamzad, with the common Persian name “marzeh khuzestani”, is an endemic medicinal plant, distributed in the southwestern areas of Iran. Salicylic acid (SA) is a signaling molecule and a hormone-like substance that plays an important role in the plant physiological processes. Objective: This study was conducted to determine the influence of foliar SA application (0, 50, 100, 200 and 400 mg.L-1) at two times including vegetative stage (VS) and both vegetative and reproductive stages (VS+RS) on growth parameters, enzymes activity including superoxide dismutase (SOD) and polyphenol oxidase (PPO), prptein content, essential oil percentage and composition of S. khuzistanica under field conditions. Methods: The essential oils were isolated from aerial flowering parts of the plants by hydro-distillation method and then subjected to GC and GC-MS analyses to determine the oil constituents. Results: Results showed that SA application at 100 and 200 mg.L-1 were the most effective treatments in growth characteristics, but the highest essential oil content and yield was obtained at 400 mg.L-1 SA treatment. In both spraying times, plants treated with 100 and 200 mg.L-1 SA concentration showed more PPO and SOD activity than control plants, respectively. Also, results showed that the 14 compositions were identified in essential oil of plants under all employed treatments. Carvacrol was the major component of oils, which is also showed more variability than that of other components. Conclusion: It was concluded that foliar spray of SA at low concentration once at vegetative and second time at reproductive stage might be employed for enhancing both primary and secondary metabolites production of S. khuzistanica plants.
Satureja khuzistanica, Carvacrol, Essential oil, Growth, Salicylic acid
Satureja khuzistanica Carvacrol, Essential oil, Growth, Salicylic acid
70
82
http://jmp.ir/browse.php?a_code=A-10-69-2&slc_lang=en&sid=1
2013/07/212013/07/292013/05/42013/04/272013/06/12013/06/122013/05/18
1392/2/28
2013/10/52013/09/292013/07/92013/08/72013/08/72013/09/142013/07/21
1392/4/30
F
Sadeghian
Department of Horticulture, Islamic Azad University – Karaj Branch
F
Sadeghian
00319475328460042056
00319475328460042056
No
Department of Horticulture, Islamic Azad University – Karaj Branch
J
Hadian
Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G. C., Evin
J
Hadian
00319475328460042057
00319475328460042057
No
Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G. C., Evin
M
Hadavi
Department of Horticulture, Islamic Azad University – Karaj Branch
M
Hadavi
00319475328460042058
00319475328460042058
No
Department of Horticulture, Islamic Azad University – Karaj Branch
A
Mohamadi
Department of Plant Physiology, College of Sciences, University of Tehran
A
Mohamadi
00319475328460042059
00319475328460042059
No
Department of Plant Physiology, College of Sciences, University of Tehran
M
Ghorbanpour
Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University
M
Ghorbanpour
m_ghorbanpour@yahoo.com
00319475328460042060
00319475328460042060
Yes
Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University
R
Ghafarzadegan
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
R
Ghafarzadegan
00319475328460042061
00319475328460042061
No
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
en
Changes in Essential Oil Composition and Leaf traits of Basil (Ocimum basilicum L.) Affected by Bio-stimulators / fertilizers Application
Changes in Essential Oil Composition and Leaf traits of Basil (Ocimum basilicum L.) Affected by Bio-stimulators / fertilizers Application
Background: Basil (Ocimum basilicum L.), a member of the Lamiaceae family, has traditionally been used as a medicinal plant in the treatment of headaches, coughs, diarrhea, constipation, warts, worms, and kidney malfunctions. Objective: To investigate the foliar application effects of bio-stimulators and bio-fertilizers on morphological and phytochemical traits of basil (Ocimum basilicum L.). Methods: Bio-stimulators in three commercial formulations of aminolforte, kadostim and fosnutren (with concentration of 1.5 L.ha-1) through foliar application, and bio-fertilizers in three commercial formulations of nitroxin, super-nitro plus and barvar II (with concentration of 0.5 L.ha-1) through seed inoculation were considered as two studied factors. Results: The results showed that the interaction effect of bio-stimulators and bio - fertilizers was significant (p<0.01) on all of studied parameters except of chlorophyll content (SPAD value). The highest leaf fresh weight (25.47 g/plant) and leaf dry weight (6.48 g/plant) were obtained under fosnutren and nitroxin treatment, also maximum leaf number (206.33) was recorded in aminolforte and nitroxin treatment. The highest leaf area (1302.2 mm2/plant) was observed in kadostim and nitroxin treatment. Also results showed that the highest content of essential oil (0.43%) was obtained in aminolforte and nitroxin, methyl chavicol (37.13%) in fosnutren and super-nitro plus, geranial (29.05%) and caryophylene (6.66%) in kadostim and nitroxin, and carvacrol (31.60%) in fosnutren and nitroxin treated plants. Conclusion: In general, the best treatment to improve growth and phytochemical traits of Ocimum basilicum were kadostim×nitroxin and fosnutren×nitroxin.
Background: Basil (Ocimum basilicum L.), a member of the Lamiaceae family, has traditionally been used as a medicinal plant in the treatment of headaches, coughs, diarrhea, constipation, warts, worms, and kidney malfunctions. Objective: To investigate the foliar application effects of bio-stimulators and bio-fertilizers on morphological and phytochemical traits of basil (Ocimum basilicum L.). Methods: Bio-stimulators in three commercial formulations of aminolforte, kadostim and fosnutren (with concentration of 1.5 L.ha-1) through foliar application, and bio-fertilizers in three commercial formulations of nitroxin, super-nitro plus and barvar II (with concentration of 0.5 L.ha-1) through seed inoculation were considered as two studied factors. Results: The results showed that the interaction effect of bio-stimulators and bio - fertilizers was significant (p<0.01) on all of studied parameters except of chlorophyll content (SPAD value). The highest leaf fresh weight (25.47 g/plant) and leaf dry weight (6.48 g/plant) were obtained under fosnutren and nitroxin treatment, also maximum leaf number (206.33) was recorded in aminolforte and nitroxin treatment. The highest leaf area (1302.2 mm2/plant) was observed in kadostim and nitroxin treatment. Also results showed that the highest content of essential oil (0.43%) was obtained in aminolforte and nitroxin, methyl chavicol (37.13%) in fosnutren and super-nitro plus, geranial (29.05%) and caryophylene (6.66%) in kadostim and nitroxin, and carvacrol (31.60%) in fosnutren and nitroxin treated plants. Conclusion: In general, the best treatment to improve growth and phytochemical traits of Ocimum basilicum were kadostim×nitroxin and fosnutren×nitroxin.
Ocimum basilicum L., Bio-fertilizers, Bio - stimulators, Essential Oil, Leaf traits
Ocimum basilicum L., Bio-fertilizers, Bio - stimulators, Essential Oil, Leaf traits
83
92
http://jmp.ir/browse.php?a_code=A-10-44-21&slc_lang=en&sid=1
2013/07/212013/07/292013/05/42013/04/272013/06/12013/06/122013/05/182013/07/14
1392/4/23
2013/10/52013/09/292013/07/92013/08/72013/08/72013/09/142013/07/212013/09/4
1392/6/13
A
Rahimi Shokooh
Department of Horticulture, Islamic Azad University, Science and Research Branch
Rahimi Shokooh
00319475328460042062
00319475328460042062
No
A
MR
Dehghani-Meshkani
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
MR
Dehghani-Meshkani
00319475328460042063
00319475328460042063
No
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
A
Mehrafarin
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
A
Mehrafarin
00319475328460042064
00319475328460042064
No
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
F
Khalighi-sigaroodi
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
F
Khalighi-sigaroodi
00319475328460042065
00319475328460042065
No
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
H
Naghdi Badi
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
H
Naghdi Badi
Naghdibadi@yahoo.com
00319475328460042066
00319475328460042066
Yes
Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
en
Effects of Nigella sativa L. Seed Oil in Type II Diabetic Patients: a Randomized, Double-Blind, Placebo - Controlled Clinical Trial
Effects of Nigella sativa L. Seed Oil in Type II Diabetic Patients: a Randomized, Double-Blind, Placebo - Controlled Clinical Trial
Background: Nigella sativa (N. sativa) seeds are used to treat diabetes mellitus in traditional medicine. Moreover, N. sativa oil has reduced the fasting blood glucose level in non-diabetic volunteers. Objective: The present study was undertaken to explore the possible anti-hyperglycemic effect of N. sativa oil in type II diabetic patients. Methods: A randomized clinical trial was conducted in 70 type II diabetic patients referring to Baqiyatallah Hospital. The subjects were enrolled into two groups of 35 each. One group received 2.5 ml N. sativa oil and the other group received similarly 2.5 ml mineral oil two times a day for three months. The fasting and 2 hour postprandial blood glucose, glycosylated hemoglobin (HbA1c), lipid profile, BMI (body mass index), liver and renal function test were determined at the baseline and after three months. Results: The blood levels of fasting and 2 hours postprandial glucose and HbA1c were significantly decreased in the N. sativa group compared with the placebo group at the end of the study. The BMI of the N. sativa group was decreased significantly from baseline. No side effects were reported. Conclusion: N. sativa oil improves glycemic control in type II diabetic patients without any side effects.
Background: Nigella sativa (N. sativa) seeds are used to treat diabetes mellitus in traditional medicine. Moreover, N. sativa oil has reduced the fasting blood glucose level in non-diabetic volunteers. Objective: The present study was undertaken to explore the possible anti-hyperglycemic effect of N. sativa oil in type II diabetic patients. Methods: A randomized clinical trial was conducted in 70 type II diabetic patients referring to Baqiyatallah Hospital. The subjects were enrolled into two groups of 35 each. One group received 2.5 ml N. sativa oil and the other group received similarly 2.5 ml mineral oil two times a day for three months. The fasting and 2 hour postprandial blood glucose, glycosylated hemoglobin (HbA1c), lipid profile, BMI (body mass index), liver and renal function test were determined at the baseline and after three months. Results: The blood levels of fasting and 2 hours postprandial glucose and HbA1c were significantly decreased in the N. sativa group compared with the placebo group at the end of the study. The BMI of the N. sativa group was decreased significantly from baseline. No side effects were reported. Conclusion: N. sativa oil improves glycemic control in type II diabetic patients without any side effects.
Nigella sativa, Blood glucose, Diabetes, Traditional medicine
Nigella sativa, Blood glucose, Diabetes, Traditional medicine
93
99
http://jmp.ir/browse.php?a_code=A-10-90-1&slc_lang=en&sid=1
2013/07/212013/07/292013/05/42013/04/272013/06/12013/06/122013/05/182013/07/142013/06/9
1392/3/19
2013/10/52013/09/292013/07/92013/08/72013/08/72013/09/142013/07/212013/09/42013/10/2
1392/7/10
MS
Hosseini
Religion and Medicine Research Center, Baqiyatallah University of Medical Sciences
MS
Hosseini
00319475328460042068
00319475328460042068
No
Religion and Medicine Research Center, Baqiyatallah University of Medical Sciences
SA
Mirkarimi
Religion and Medicine Research Center, Baqiyatallah University of Medical Sciences
SA
Mirkarimi
00319475328460042069
00319475328460042069
No
Religion and Medicine Research Center, Baqiyatallah University of Medical Sciences
M
Amini
Gastroentrology and Liver diseases Research Center, Baqiyatallah University of Medical Sciences
M
Amini
00319475328460042070
00319475328460042070
No
Gastroentrology and Liver diseases Research Center, Baqiyatallah University of Medical Sciences
R
Mohtashami
Religion and Medicine Research Center, Baqiyatallah University of Medical Sciences
R
Mohtashami
00319475328460042071
00319475328460042071
No
Religion and Medicine Research Center, Baqiyatallah University of Medical Sciences
S
Kianbakht
3- Pharmacology and applied Medicine Department of Medicinal Plant Research Center, Institute of Medicinal Plants, ACECR
S
Kianbakht
00319475328460042072
00319475328460042072
No
Pharmacology and applied Medicine Department of Medicinal Plant Research Center, Institute of Medicinal Plants, ACECR
H
Fallah Huseini
Pharmacology and applied Medicine Department of Medicinal Plant Research Center, Institute of Medicinal Plants, ACECR
H
Fallah Huseini
Huseini_fallah@yahoo.com
00319475328460042073
00319475328460042073
Yes
Pharmacology and applied Medicine Department of Medicinal Plant Research Center, Institute of Medicinal Plants, ACECR
en
Over - expression Effect of Gene Encoding 3-hydroxy-3-Methylglutaryl-CoA Reductase on Production of Taxol in Iranian Hazel (Corylus avellana L.)
Over - expression Effect of Gene Encoding 3-hydroxy-3-Methylglutaryl-CoA Reductase on Production of Taxol in Iranian Hazel (Corylus avellana L.)
Background: Sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol. Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession number KF306244, showed by CiHMGR) was isolated and over-expressed in pCAMBIA1304 binary vector. The effect of transient over-expression of HMGR in callus and leaf were evaluated on Taxol production. Methods: The calli was established through the culture of immature cotyledon on Murashige and Skoog basal medium supplemented with 2, 4-D and BA. The first strand cDNA of CiHMGR was synthesized by specific primers. Enzymatic assay of recombinant CiHMGR in E. coli were done by western blott and His-tag affinity techniques. Also production of taxol in transformed callus and leaf were evaluated by HPLC analysis. Results: An Open Reading Frame (ORF) with 1698 bp length and a deduced polypeptide with 566 amino acid residues were amplified. The highest and lowest amount of taxol was 0.016 mg/g.DW and 0.004 mg/gDW in transformed calli and untransformed leaves respectively. Conclusion: Generally the over-expression of HMGR increase the total isoprenoids yield, therefore to have high production of target secondary metabolites (taxol) we need both of network of transformed genes and elicited cell culture.
Background: Sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol. Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession number KF306244, showed by CiHMGR) was isolated and over-expressed in pCAMBIA1304 binary vector. The effect of transient over-expression of HMGR in callus and leaf were evaluated on Taxol production. Methods: The calli was established through the culture of immature cotyledon on Murashige and Skoog basal medium supplemented with 2, 4-D and BA. The first strand cDNA of CiHMGR was synthesized by specific primers. Enzymatic assay of recombinant CiHMGR in E. coli were done by western blott and His-tag affinity techniques. Also production of taxol in transformed callus and leaf were evaluated by HPLC analysis. Results: An Open Reading Frame (ORF) with 1698 bp length and a deduced polypeptide with 566 amino acid residues were amplified. The highest and lowest amount of taxol was 0.016 mg/g.DW and 0.004 mg/gDW in transformed calli and untransformed leaves respectively. Conclusion: Generally the over-expression of HMGR increase the total isoprenoids yield, therefore to have high production of target secondary metabolites (taxol) we need both of network of transformed genes and elicited cell culture.
Corylus avellana, Diterpenoids, Elicitor, Mevalonate biosynthesis pathway
Corylus avellana, Diterpenoids, Elicitor, Mevalonate biosynthesis pathway
100
110
http://jmp.ir/browse.php?a_code=A-10-75-2&slc_lang=en&sid=1
2013/07/212013/07/292013/05/42013/04/272013/06/12013/06/122013/05/182013/07/142013/06/92013/06/22
1392/4/1
2013/10/52013/09/292013/07/92013/08/72013/08/72013/09/142013/07/212013/09/42013/10/22013/07/10
1392/4/19
A
Qaderi
Department of Plant Breeding, Science and Research Branch, Islamic Azad University
A
Qaderi
Ardeshir582003@yahoo.com
00319475328460042074
00319475328460042074
Yes
Department of Plant Breeding, Science and Research Branch, Islamic Azad University
M
Omidi
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Tehran University
M
Omidi
00319475328460042075
00319475328460042075
No
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Tehran University
AR
Zebarjadi
Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University
AR
Zebarjadi
00319475328460042076
00319475328460042076
No
Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University
R
Hajiaghaee
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
R
Hajiaghaee
00319475328460042077
00319475328460042077
No
Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR