Journal of Medicinal Plants
فصلنامه گياهان دارویی
J. Med. Plants
Medical Sciences
http://jmp.ir
1
admin
2717-204X
2717-2058
10.61186/jmp
14
8888
13
en
jalali
1387
11
1
gregorian
2009
2
1
8
29
online
1
fulltext
en
Cytotoxic Flavonoid from <i>Achillea talagonica</i> Bioss.
Cytotoxic Flavonoid from <i>Achillea talagonica</i> Bioss.
فارماكوگنوزی و فارماسيوتيكس
Pharmacognosy & Pharmaceutics
پژوهشی
Research
<p dir="ltr" style="text-align: justify;"><strong>Background:</strong> <i>Achillea talagonica </i>(Asteraceae) is an endemic herbaceous plant in Iran with small yellow flowers and several times pinnately divided leaves in worm shape. The plant was found to be cytotoxic in our previous studies.</p>
<p dir="ltr" style="text-align: justify;"><strong>Materials and Methods:</strong> <i>A. talagonica</i> was collected in May 2005 from Talegan in north of Iran during the full flowering stage. Dried aerial parts of the plant were reduced in to small pieces and percolated with ethyl acetate for 72 hours. The extract obtained was filtered and then concentrated under reduced pressure and completely dried by a freeze dryer. Column chromatography of the extract on silica gel and sephadex LH-20 yielded in isolation of three main components. Cytotoxic activity was evaluated against the <i>Artemia salina</i> larvae by the Brine Shrimp Cytotoxicity Bioassay.</p>
<p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Isolated compounds were identified as β-sitosterol (1), salvigenin (5- hydroxy 4', 6, 7- three methoxy flavone) (2) and santoflavon (5- hydroxy 4', 3', 6, 7- tetra methoxy flavone) (3). NMR data of all the isolated compounds showed good agreement with literature data.</p>
<div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> In our previous studies ethyl acetate extract of <i>A. talagonica</i> showed cytotoxic activity in brine shrimp lethality assay. The results of BST assay indicated that only santoflavone (3) showed a good cytotoxic activity (LC<sub>50 </sub>= 15 μ g/ml) against <i>A. salina</i> larvae so this compound seems to be responsible for the extract toxicity of <i>A. talagonica</i> against <i>Artemia</i> nauplii.</div>
<p dir="ltr" style="text-align: justify;"><strong>Background:</strong> <i>Achillea talagonica </i>(Asteraceae) is an endemic herbaceous plant in Iran with small yellow flowers and several times pinnately divided leaves in worm shape. The plant was found to be cytotoxic in our previous studies.</p>
<p dir="ltr" style="text-align: justify;"><strong>Materials and Methods:</strong> <i>A. talagonica</i> was collected in May 2005 from Talegan in north of Iran during the full flowering stage. Dried aerial parts of the plant were reduced in to small pieces and percolated with ethyl acetate for 72 hours. The extract obtained was filtered and then concentrated under reduced pressure and completely dried by a freeze dryer. Column chromatography of the extract on silica gel and sephadex LH-20 yielded in isolation of three main components. Cytotoxic activity was evaluated against the <i>Artemia salina</i> larvae by the Brine Shrimp Cytotoxicity Bioassay.</p>
<p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Isolated compounds were identified as β-sitosterol (1), salvigenin (5- hydroxy 4', 6, 7- three methoxy flavone) (2) and santoflavon (5- hydroxy 4', 3', 6, 7- tetra methoxy flavone) (3). NMR data of all the isolated compounds showed good agreement with literature data.</p>
<div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> In our previous studies ethyl acetate extract of <i>A. talagonica</i> showed cytotoxic activity in brine shrimp lethality assay. The results of BST assay indicated that only santoflavone (3) showed a good cytotoxic activity (LC<sub>50 </sub>= 15 μ g/ml) against <i>A. salina</i> larvae so this compound seems to be responsible for the extract toxicity of <i>A. talagonica</i> against <i>Artemia</i> nauplii.</div>
Asteraceae, <,i>,Achillea talagonica<,/i>,, Santoflavone, Salvigenin, Cytotoxic
Asteraceae, <,i>,Achillea talagonica<,/i>,, Santoflavone, Salvigenin, Cytotoxic
52
56
http://jmp.ir/browse.php?a_code=A-10-161-4&slc_lang=en&sid=1
S
Saeidnia
S
Saeidnia
soodabehsaeidnia@hotmail.com
100319475328460038530
100319475328460038530
Yes
Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran
Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran
F
Moradi-Afrapoli
F
Moradi-Afrapoli
100319475328460038531
100319475328460038531
No
Department of Pharmacognosy, Faculty of Pharmacy, Medicinal Sciences, University of Tehran, Tehran
Department of Pharmacognosy, Faculty of Pharmacy, Medicinal Sciences, University of Tehran, Tehran
AR
Gohari
AR
Gohari
100319475328460038532
100319475328460038532
No
Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran
Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran
M
Malmir
M
Malmir
100319475328460038533
100319475328460038533
No
Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran,
Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran,