Journal of Medicinal Plants
فصلنامه گياهان دارویی
J. Med. Plants
Medical Sciences
http://jmp.ir
1
admin
2717-204X
2717-2058
10.61186/jmp
14
8888
13
en
jalali
1388
12
1
gregorian
2010
3
1
9
33
online
1
fulltext
en
Dual Effects of Plant Antioxidants on Neuron Cell Viability
Dual Effects of Plant Antioxidants on Neuron Cell Viability
فارماكوگنوزی و فارماسيوتيكس
Pharmacognosy & Pharmaceutics
پژوهشی
Research
<div dir="ltr" style="text-align: justify;">
<p style="text-align: justify;"><strong>Background:</strong> Many studies have focused on oxidative stress induced damage and hence, the protective effects conferred by antioxidants. An example is neurodegenerative diseases which is thought to occur due to neuronal loss associated with oxidative stress. However, some antioxidants such as vitamin E have been shown to also exert pro-oxidative effects at high concentration.</p>
<p style="text-align: justify;"><strong>Objective:</strong> In this study the cytotoxicity and neuroprotective potentials of <i>Chlorella vulgaris</i> (CV), <i>Momordica charantia </i>(MC) and <i>Piper betle</i> (PB) were investigated and correlated with the antioxidant potential. Tocotrienol Rich Fraction (TRF) served as positive control since it had been shown previously to have high antioxidant potential as well as to exert neuroprotective and neurocytotoxic effects.</p>
<p style="text-align: justify;"><strong>Method:</strong> Free radical scavenging activities of hot water extract of CV, aqueous extract of MC, aqueous extract of PB and TRF were determined by using DPPH (1, 1-diphenyl-2-picryl-hydrazyl) assay. Cytotoxicity and neuroprotective effects were measured by using 3 - (4, 5 -dimethylthiazol-2-yl) -5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) against BSO-induced neuron cell death.</p>
<p style="text-align: justify;"><strong>Results:</strong> Results showed that TRF has the highest radical scavenging activity followed PB> MC> CV. The MTS results showed that TRF (1-50 µg/ml) as positive control, PB (0.001-100µg/ml) and MC (1-500µg/ml) conferred significant protection against BSO-induced cell death. These plants were cytotoxic at high concentrations. However CV extract did not show significant neuroprotective effect against BSO-induced cell death nor cytotoxic effect.</p>
<div style="text-align: justify;"><strong>Conclusion:</strong> The present findings showed that plant extracts with the higher free radical scavenging activity showed neuroprotective effects at low concentrations but were cytotoxic at higher concentrations.</div>
</div>
<p style="text-align: justify;"><strong>Background:</strong> Many studies have focused on oxidative stress induced damage and hence, the protective effects conferred by antioxidants. An example is neurodegenerative diseases which is thought to occur due to neuronal loss associated with oxidative stress. However, some antioxidants such as vitamin E have been shown to also exert pro-oxidative effects at high concentration.</p>
<p style="text-align: justify;"><strong>Objective:</strong> In this study the cytotoxicity and neuroprotective potentials of <i>Chlorella vulgaris</i> (CV), <i>Momordica charantia </i>(MC) and <i>Piper betle</i> (PB) were investigated and correlated with the antioxidant potential. Tocotrienol Rich Fraction (TRF) served as positive control since it had been shown previously to have high antioxidant potential as well as to exert neuroprotective and neurocytotoxic effects.</p>
<p style="text-align: justify;"><strong>Method:</strong> Free radical scavenging activities of hot water extract of CV, aqueous extract of MC, aqueous extract of PB and TRF were determined by using DPPH (1, 1-diphenyl-2-picryl-hydrazyl) assay. Cytotoxicity and neuroprotective effects were measured by using 3 - (4, 5 -dimethylthiazol-2-yl) -5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) against BSO-induced neuron cell death.</p>
<p style="text-align: justify;"><strong>Results:</strong> Results showed that TRF has the highest radical scavenging activity followed PB> MC> CV. The MTS results showed that TRF (1-50 µg/ml) as positive control, PB (0.001-100µg/ml) and MC (1-500µg/ml) conferred significant protection against BSO-induced cell death. These plants were cytotoxic at high concentrations. However CV extract did not show significant neuroprotective effect against BSO-induced cell death nor cytotoxic effect.</p>
<div style="text-align: justify;"><strong>Conclusion:</strong> The present findings showed that plant extracts with the higher free radical scavenging activity showed neuroprotective effects at low concentrations but were cytotoxic at higher concentrations.</div>
Antioxidant, <,i>,Centella asiatica<,/i>,, <,i>,Chlorella vulgaris<,/i>,, <,i>,Momordica charantia<,/i>,, Neuroprotection, <,i>,Piper betl<,/i>,
Antioxidant, <,i>,Centella asiatica<,/i>,, <,i>,Chlorella vulgaris<,/i>,, <,i>,Momordica charantia<,/i>,, Neuroprotection, <,i>,Piper betl<,/i>,
113
123
http://jmp.ir/browse.php?a_code=A-10-413-1&slc_lang=en&sid=1
SO
Norfaizatul
نورفازاتول
100319475328460038150
100319475328460038150
No
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
CZ
Zetty Akmal
زتی آکمل
100319475328460038151
100319475328460038151
No
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
AK
Noralisa
نورالیسا
100319475328460038152
100319475328460038152
No
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
SM
Then
تن
100319475328460038153
100319475328460038153
No
UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia
UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia
WN
Wan Zurinah
وان زوریناه
100319475328460038154
100319475328460038154
No
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia, UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia, UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia
M
Musalmah
موسالماه
musalmah@medic.ukm.my
100319475328460038155
100319475328460038155
Yes
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia