Journal of Medicinal Plants
فصلنامه گياهان دارویی
J. Med. Plants
Medical Sciences
http://jmp.ir
1
admin
2717-204X
2717-2058
10.61186/jmp
14
8888
13
en
jalali
1388
12
1
gregorian
2010
3
1
9
33
online
1
fulltext
en
Identification and Quantitative Determination of 4-Hydroxyisoleucine in <i>Trigonella foenum-graecum</i> L. from Iran
Identification and Quantitative Determination of 4-Hydroxyisoleucine in <i>Trigonella foenum-graecum</i> L. from Iran
فارماكوگنوزی و فارماسيوتيكس
Pharmacognosy & Pharmaceutics
پژوهشی
Research
<p dir="ltr" style="text-align: justify;"> <strong> Background:</strong> The seeds of <i>Trigonella foenum-graecum </i>L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine.</p>
<p dir="ltr" style="text-align: justify;"> <strong>Objective:</strong> In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated.</p>
<p dir="ltr" style="text-align: justify;"> <strong>Methods:</strong> Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique.</p>
<p dir="ltr" style="text-align: justify;"> <strong> Results:</strong> The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran.</p>
<div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> According to the effects of 4-hydroxyisoleucine such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders.</div>
<p style="text-align: justify;"> <strong> Background:</strong> The seeds of <i>Trigonella foenum-graecum </i>L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine.</p>
<p style="text-align: justify;"> <strong>Objective:</strong> In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated.</p>
<p style="text-align: justify;"> <strong>Methods:</strong> Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique.</p>
<p style="text-align: justify;"> <strong> Results:</strong> The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran.</p>
<div style="text-align: justify;"><strong>Conclusion:</strong> According to the effects of 4-hydroxyisoleucine such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders.</div>
<,i>,Trigonella foenum-graecum<,/i>,, Amino acid, Diabetes, 4-hydroxyisoleucine
<,i>,Trigonella foenum-graecum<,/i>,, Amino acid, Diabetes, 4-hydroxyisoleucine
29
34
http://jmp.ir/browse.php?a_code=A-10-319-1&slc_lang=en&sid=1
H
Hajimehdipoor
هما
حاجی مهدی پور
100319475328460038064
100319475328460038064
No
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran
SE
Sadat-Ebrahimi
سیدابراهیم
سادات ابراهیمی
sesebrahimi@sina.tums.ac.ir
100319475328460038065
100319475328460038065
Yes
Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
Y
Amanzadeh
امن زاده
100319475328460038066
100319475328460038066
No
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
M
Izaddoost
محمد
ایزددوست
100319475328460038067
100319475328460038067
No
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
E
Givi
گیوی
100319475328460038068
100319475328460038068
No
Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran