Journal of Medicinal Plants
فصلنامه گياهان دارویی
J. Med. Plants
Medical Sciences
http://jmp.ir
1
admin
2717-204X
2717-2058
10.61186/jmp
14
8888
13
en
jalali
1389
6
1
gregorian
2010
9
1
9
35
online
1
fulltext
fa
Essential Oils from Hairy Root Cultures and Field Cultivated Roots of Valerian (<i>Valeriana sisymbriifolium</i>)
Essential Oils from Hairy Root Cultures and Field Cultivated Roots of Valerian (<i>Valeriana sisymbriifolium</i>)
فارماكوگنوزی و فارماسيوتيكس
Pharmacognosy & Pharmaceutics
پژوهشی
Research
<div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Plant cell cultivations are considered as an alternative to agricultural processes for producing valuable phytochemicals (secondary metabolites). The use of plant cell cultures has overcome several inconveniences for the production of secondary metabolites. <strong>Objective:</strong> The essential oils isolated from roots of 24-month-old field grown valerian (<em>Valeriana sisymbriifolium</em>) and hairy root cultures were analysed by GC and GC-mass spectrometry. <strong>Methods:</strong> Transformed root cultures of valerian were established by inoculation of sterile plantlets grown with <em>Agrobacterium rhizogenes</em> strain R1601. Qualitative and quantitative differences were found between the essential oils from the non-transformed roots and those from the hairy roots. <strong>Results:</strong> There are some major differences in the hairy root culture and field grown valerian essential oils especially in valeranone, valerenal, valerenyl acetate, valerenic acid and valerenol compounds. The essential oils from the plant roots were obtained in a yield of 13.2% bornyl acetate, valerenal 12.7%, -Pinene 7.50 compared with that from transformed root culture identified as kessyi alcohol (10.10%) and kessyl acetate (9.90%), as the main constituents. <strong>Conclusion:</strong> Inoculation with <em>Agrobacterium rhizogenes</em> strain R1601 was found to be an effective means of inducing hairy root formation on <em>Valeriana sisymbriifolium</em>.</div>
<div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Plant cell cultivations are considered as an alternative to agricultural processes for producing valuable phytochemicals (secondary metabolites). The use of plant cell cultures has overcome several inconveniences for the production of secondary metabolites. <strong>Objective:</strong> The essential oils isolated from roots of 24-month-old field grown valerian (<em>Valeriana sisymbriifolium</em>) and hairy root cultures were analysed by GC and GC-mass spectrometry. <strong>Methods:</strong> Transformed root cultures of valerian were established by inoculation of sterile plantlets grown with <em>Agrobacterium rhizogenes</em> strain R1601. Qualitative and quantitative differences were found between the essential oils from the non-transformed roots and those from the hairy roots. <strong>Results:</strong> There are some major differences in the hairy root culture and field grown valerian essential oils especially in valeranone, valerenal, valerenyl acetate, valerenic acid and valerenol compounds. The essential oils from the plant roots were obtained in a yield of 13.2% bornyl acetate, valerenal 12.7%, -Pinene 7.50 compared with that from transformed root culture identified as kessyi alcohol (10.10%) and kessyl acetate (9.90%), as the main constituents. <strong>Conclusion:</strong> Inoculation with <em>Agrobacterium rhizogenes</em> strain R1601 was found to be an effective means of inducing hairy root formation on <em>Valeriana sisymbriifolium</em>.</div>
<,i>,Valeriana sisymbriifolium<,/i>,, <,i>,Agrobacterium rhizogenes<,/i>,, Hairy root culture, Essential oil
<,i>,Valeriana sisymbriifolium<,/i>,, <,i>,Agrobacterium rhizogenes<,/i>,, Hairy root culture, Essential oil
120
128
http://jmp.ir/browse.php?a_code=A-10-199-1&slc_lang=fa&sid=1
Y
Filizadeh
Y
Filizadeh
filizadeh@shahed.ac.ir
100319475328460037862
100319475328460037862
Yes
Shahed Medicinal Plants Research Center
Shahed Medicinal Plants Research Center
G
Goodarzi
G
Goodarzi
100319475328460037863
100319475328460037863
No
Shahed Medicinal Plants Research Center
Shahed Medicinal Plants Research Center