Journal of Medicinal Plants

en Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <div dir="ltr" style="text-align: justify;">Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes. Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared. Methods: Glucose uptake measurement was performed using 2-(N-(7-nitrobenzin-2-oxa-1, 3- diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) and Omega FluoStar plate reader. Incubation of cells (104/ml, in 24 well plate at 37 °C in 5% CO2 / air) with three phytoestrogens at concentration of 10-4 M to 10-9M in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested. Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 µM, which stimulated uptake about three-fold compared with control. Longer treatments with 10-4M to 10-6 M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA.</div> <div dir="ltr" style="text-align: justify;">Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes. Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared. Methods: Glucose uptake measurement was performed using 2-(N-(7-nitrobenzin-2-oxa-1, 3- diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) and Omega FluoStar plate reader. Incubation of cells (104/ml, in 24 well plate at 37 °C in 5% CO2 / air) with three phytoestrogens at concentration of 10-4 M to 10-9M in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested. Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 µM, which stimulated uptake about three-fold compared with control. Longer treatments with 10-4M to 10-6 M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA.</div> Phytoestrogens, Hyperglycemia, In vitro, study, 2-NBDG Phytoestrogens, Hyperglycemia, In vitro, study, 2-NBDG 145 152 http://jmp.ir/browse.php?a_code=A-10-1331-1&slc_lang=en&sid=1 D Roostaei D Roostaei 100319475328460041506 100319475328460041506 No Faculty of Life Sciences and Computing, London Metropolitan University, London, UK Faculty of Life Sciences and Computing, London Metropolitan University, London, UK Sh Rezazadeh Sh Rezazadeh 100319475328460041507 100319475328460041507 No Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran S Sharafi S Sharafi 100319475328460041508 100319475328460041508 No The Royal College of General Practitioners (RCGP), London, UK The Royal College of General Practitioners (RCGP), London, UK K White K White Kenneth.white@londonmet.ac.uk 100319475328460041509 100319475328460041509 Yes Faculty of Life Sciences and Computing, London Metropolitan University, London, UK Faculty of Life Sciences and Computing, London Metropolitan University, London, UK