Journal of Medicinal Plants

en Phytochemical and Antioxidant Activities of Berberis integerrima and Berberis vulgaris and Pharmacological Effects of the more Active Species on Alloxan-Induced Diabetic Rats Phytochemical and Antioxidant Activities of Berberis integerrima and Berberis vulgaris and Pharmacological Effects of the more Active Species on Alloxan-Induced Diabetic Rats فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research Background: Medicinal plants with biologically active components such as antioxidant and antidiabetic are useful sources of novel therapeutics. In Iran, traditionally believed that the aqueous extract of berberry fruit (Berberis integerima F.) improved health, especially in people with diabetes. Objective: In this study, in vitro phytochemical and antioxidant activities of B. integerrima and B. vulgaris fruit aqueous extracts (BIFA and BVFA) were compared. Effects of more active species on fasting blood sugar (FBS), serum lipid, and malondialdehyde (MDA) were evaluated in alloxan-induced diabetic rats. Methods: The phytochemical potential and antioxidant activity of these extracts were quantified in terms of total phenolic content and total reducing power, respectively. BIFA (500 mg/kg) and glibenclamide (2 mg/kg) were administered orally to alloxan-induced diabetic rats and FBS, body weight, lipid profile, and serum MDA were monitored at 0, 7, 14, and 21 days after induction of diabetes. Results: BIFA showed the highest phenolic content (6.816 ± 0.132 mg/g of dry extract) and antioxidant activity (0.134 ± 0.022 EC50 mg/mL). LD50 of BIFA was found to be ˃2500 mg/kg. FBS, lipid profile, and serum MDA have been significantly reduced in BIFA-treated rats after 21 days versus diabetic control and glibenclamide-treated rats. Extract has significantly decreased FBS levels of rats from 138.1 ± 1.68 to 82.00 ± 4.02 mg/dL. Furthermore, body weight has significantly improved in treated groups. Conclusion: The results indicate B. integerrima with high phytochemical and antioxidant activities, has the potential of suppressed hyperglycemia, hyperlipidemia and lipid peroxidation. Background: Medicinal plants with biologically active components such as antioxidant and antidiabetic are useful sources of novel therapeutics. In Iran, traditionally believed that the aqueous extract of berberry fruit (Berberis integerima F.) improved health, especially in people with diabetes. Objective: In this study, in vitro phytochemical and antioxidant activities of B. integerrima and B. vulgaris fruit aqueous extracts (BIFA and BVFA) were compared. Effects of more active species on fasting blood sugar (FBS), serum lipid, and malondialdehyde (MDA) were evaluated in alloxan-induced diabetic rats. Methods: The phytochemical potential and antioxidant activity of these extracts were quantified in terms of total phenolic content and total reducing power, respectively. BIFA (500 mg/kg) and glibenclamide (2 mg/kg) were administered orally to alloxan-induced diabetic rats and FBS, body weight, lipid profile, and serum MDA were monitored at 0, 7, 14, and 21 days after induction of diabetes. Results: BIFA showed the highest phenolic content (6.816 ± 0.132 mg/g of dry extract) and antioxidant activity (0.134 ± 0.022 EC50 mg/mL). LD50 of BIFA was found to be ˃2500 mg/kg. FBS, lipid profile, and serum MDA have been significantly reduced in BIFA-treated rats after 21 days versus diabetic control and glibenclamide-treated rats. Extract has significantly decreased FBS levels of rats from 138.1 ± 1.68 to 82.00 ± 4.02 mg/dL. Furthermore, body weight has significantly improved in treated groups. Conclusion: The results indicate B. integerrima with high phytochemical and antioxidant activities, has the potential of suppressed hyperglycemia, hyperlipidemia and lipid peroxidation. Antioxidant activity, Fasting Blood Sugar, Glibenclamide, Hyperglycemia, Phytochemical Antioxidant activity, Fasting Blood Sugar, Glibenclamide, Hyperglycemia, Phytochemical 111 121 http://jmp.ir/browse.php?a_code=A-10-958-4&slc_lang=en&sid=1 M Bayani M Bayani 100319475328460041495 100319475328460041495 No Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Faculty of Veterinary Medicine, Semnan University, Semnan, Iran M Ahmadi-hamedani M Ahmadi-hamedani m.ahmadi@profs.semnan.ac.ir 100319475328460041496 100319475328460041496 Yes Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran A Jebelli Javan A Jebelli Javan 100319475328460041497 100319475328460041497 No Department of Food Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Department of Food Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran