Journal of Medicinal Plants
فصلنامه گياهان دارویی
J. Med. Plants
Medical Sciences
http://jmp.ir
1
admin
2717-204X
2717-2058
10.61186/jmp
14
8888
13
en
jalali
1395
5
1
gregorian
2016
8
1
15
59
online
1
fulltext
en
Improving Trigonelline Production in Hairy Root Culture of Fenugreek (<i>Trigonella foenum-graecum</i>)
Improving Trigonelline Production in Hairy Root Culture of Fenugreek (<i>Trigonella foenum-graecum</i>)
بيوتكنولوژی
Biotechnology
پژوهشی
Research
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Background:</strong> <em>Trigonella foenum-graecum</em> L. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Objective:</strong> In this study, hairy roots culture as a novel method for trigonelline production was evaluated.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Methods:</strong> For optimizing the hairy roots culture of <em>Trigonella foenum-graecum,</em> three strains of <em>Agrobacterium rhyzogenes</em> (ATCC15834, MSU440 and K599) via two inoculations methods including scotch and vacuum pump were used to agro-infiltration. Two elicitors including methyl jasmonate (0, 25, 50, 100 and 200 µM) and chitosan (0, 50, 100, 150 and 200mglˉ') were added to liquid medium as abiotic and biotic elicitors in various concentrations, respectively.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Results:</strong> The trigonelline content was increased via elicitation by methyl jasmonate and chitosan against control condition. The maximum trigonelline (36.7 and 37.3 mM/g D.W) were observed in 100 µM of methyl jasmonate and 150 mg/l of chitosan, respectively.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Conclusion:</strong> All parts of the seedling (crown, stem and leaf) were able to produce the hairy roots. Also, the highest dry weight of hairy root was obtained by <em>A. rhizogenes</em> strain 15834. The transformation of fenugreek using <em>Agrobacterium rhizogenes</em> to form hairy root cultures has the potential benefits of fast growth and rates of secondary metabolite production equal to or greater than that found for the intact plant.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Background:</strong> <em>Trigonella foenum-graecum</em> L. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Objective:</strong> In this study, hairy roots culture as a novel method for trigonelline production was evaluated.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Methods:</strong> For optimizing the hairy roots culture of <em>Trigonella foenum-graecum,</em> three strains of <em>Agrobacterium rhyzogenes</em> (ATCC15834, MSU440 and K599) via two inoculations methods including scotch and vacuum pump were used to agro-infiltration. Two elicitors including methyl jasmonate (0, 25, 50, 100 and 200 µM) and chitosan (0, 50, 100, 150 and 200mglˉ') were added to liquid medium as abiotic and biotic elicitors in various concentrations, respectively.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Results:</strong> The trigonelline content was increased via elicitation by methyl jasmonate and chitosan against control condition. The maximum trigonelline (36.7 and 37.3 mM/g D.W) were observed in 100 µM of methyl jasmonate and 150 mg/l of chitosan, respectively.</p>
<p dir="ltr" style="margin-right: 9.05pt; text-align: justify;"><strong>Conclusion:</strong> All parts of the seedling (crown, stem and leaf) were able to produce the hairy roots. Also, the highest dry weight of hairy root was obtained by <em>A. rhizogenes</em> strain 15834. The transformation of fenugreek using <em>Agrobacterium rhizogenes</em> to form hairy root cultures has the potential benefits of fast growth and rates of secondary metabolite production equal to or greater than that found for the intact plant.</p>
,Trigonella foenum-graecum L., Hairy Root Culture, Trigonelline, Chitosan, Methyl jasmonate
Trigonella foenum-graecum L., Hairy Root Culture, Trigonelline, Chitosan, Methyl jasmonate
73
80
http://jmp.ir/browse.php?a_code=A-10-44-101&slc_lang=en&sid=1
A
Qaderi
اردشیر
قادری
ardeshir582008@gmail.com
100319475328460041479
100319475328460041479
No
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاددانشگاهی، کرج، ایران
Z
Akbari
اکبری
100319475328460041480
100319475328460041480
No
Science and Research Branch, Islamic Azad University, Department of Horticultural Tehran, Iran
دانشگاه آزاد اسلامی واحد علوم تحقیقات
S
Kalateh-jari
کلاتهجاری
100319475328460041481
100319475328460041481
No
Science and Research Branch, Islamic Azad University, Department of Horticultural Tehran, Iran
دانشگاه آزاد اسلامی واحد علوم تحقیقات
F
Fatehi
فؤاد
فاتحی
100319475328460041482
100319475328460041482
No
Payame Noor University (PNU), Tehran
دانشگاه پیام نور تهران
M
Tolyat
سیدمجید
تولیت
toliat@acecr.ac.ir
100319475328460041483
100319475328460041483
No
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاددانشگاهی، کرج، ایران
M
Jalali Moghadam
جلالی مقدم
100319475328460041484
100319475328460041484
No
Payame Noor University (PNU), P.O.BOX: 19395-3697, Tehran
دانشگاه پیام نور تهران
H
Naghdi Badi
حسنعلی
نقدیبادی
Naghdibadi@yahoo.com
100319475328460041485
100319475328460041485
Yes
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاددانشگاهی، کرج، ایران