Journal of Medicinal Plants

en Improving Trigonelline Production in Hairy Root Culture of Fenugreek (Trigonella foenum-graecum) Improving Trigonelline Production in Hairy Root Culture of Fenugreek (Trigonella foenum-graecum) بيوتكنولوژی Biotechnology پژوهشی Research Background: Trigonella foenum-graecum L. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. Objective: In this study, hairy roots culture as a novel method for trigonelline production was evaluated. Methods: For optimizing the hairy roots culture of Trigonella foenum-graecum, three strains of Agrobacterium rhyzogenes (ATCC15834, MSU440 and K599) via two inoculations methods including scotch and vacuum pump were used to agro-infiltration. Two elicitors including methyl jasmonate (0, 25, 50, 100 and 200 µM) and chitosan (0, 50, 100, 150 and 200mglˉ') were added to liquid medium as abiotic and biotic elicitors in various concentrations, respectively. Results: The trigonelline content was increased via elicitation by methyl jasmonate and chitosan against control condition. The maximum trigonelline (36.7 and 37.3 mM/g D.W) were observed in 100 µM of methyl jasmonate and 150 mg/l of chitosan, respectively. Conclusion: All parts of the seedling (crown, stem and leaf) were able to produce the hairy roots. Also, the highest dry weight of hairy root was obtained by A. rhizogenes strain 15834. The transformation of fenugreek using Agrobacterium rhizogenes to form hairy root cultures has the potential benefits of fast growth and rates of secondary metabolite production equal to or greater than that found for the intact plant. Background: Trigonella foenum-graecum L. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. Objective: In this study, hairy roots culture as a novel method for trigonelline production was evaluated. Methods: For optimizing the hairy roots culture of Trigonella foenum-graecum, three strains of Agrobacterium rhyzogenes (ATCC15834, MSU440 and K599) via two inoculations methods including scotch and vacuum pump were used to agro-infiltration. Two elicitors including methyl jasmonate (0, 25, 50, 100 and 200 µM) and chitosan (0, 50, 100, 150 and 200mglˉ') were added to liquid medium as abiotic and biotic elicitors in various concentrations, respectively. Results: The trigonelline content was increased via elicitation by methyl jasmonate and chitosan against control condition. The maximum trigonelline (36.7 and 37.3 mM/g D.W) were observed in 100 µM of methyl jasmonate and 150 mg/l of chitosan, respectively. Conclusion: All parts of the seedling (crown, stem and leaf) were able to produce the hairy roots. Also, the highest dry weight of hairy root was obtained by A. rhizogenes strain 15834. The transformation of fenugreek using Agrobacterium rhizogenes to form hairy root cultures has the potential benefits of fast growth and rates of secondary metabolite production equal to or greater than that found for the intact plant. ,Trigonella foenum-graecum L., Hairy Root Culture, Trigonelline, Chitosan, Methyl jasmonate Trigonella foenum-graecum L., Hairy Root Culture, Trigonelline, Chitosan, Methyl jasmonate 73 80 http://jmp.ir/browse.php?a_code=A-10-44-101&slc_lang=en&sid=1 A Qaderi اردشیر قادری ardeshir582008@gmail.com 100319475328460041479 100319475328460041479 No Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاددانشگاهی، کرج، ایران Z Akbari اکبری 100319475328460041480 100319475328460041480 No Science and Research Branch, Islamic Azad University, Department of Horticultural Tehran, Iran دانشگاه آزاد اسلامی واحد علوم تحقیقات S Kalateh-jari کلاته‌جاری 100319475328460041481 100319475328460041481 No Science and Research Branch, Islamic Azad University, Department of Horticultural Tehran, Iran دانشگاه آزاد اسلامی واحد علوم تحقیقات F Fatehi فؤاد فاتحی 100319475328460041482 100319475328460041482 No Payame Noor University (PNU), Tehran دانشگاه پیام نور تهران M Tolyat سیدمجید تولیت toliat@acecr.ac.ir 100319475328460041483 100319475328460041483 No Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاددانشگاهی، کرج، ایران M Jalali Moghadam جلالی مقدم 100319475328460041484 100319475328460041484 No Payame Noor University (PNU), P.O.BOX: 19395-3697, Tehran دانشگاه پیام نور تهران H Naghdi Badi حسنعلی نقدی‌بادی Naghdibadi@yahoo.com 100319475328460041485 100319475328460041485 Yes Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاددانشگاهی، کرج، ایران