TY - JOUR JF - jmpir JO - J. Med. Plants VL - 9 IS - 33 PY - 2010 Y1 - 2010/3/01 TI - Identification and Quantitative Determination of 4-Hydroxyisoleucine in Trigonella foenum-graecum L. from Iran TT - Identification and Quantitative Determination of 4-Hydroxyisoleucine in Trigonella foenum-graecum L. from Iran N2 - Background: The seeds of Trigonella foenum-graecum L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine. Objective: In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated. Methods: Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique. Results: The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran. Conclusion: According to the effects of 4-hydroxyisoleucine such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders. SP - 29 EP - 34 AU - Hajimehdipoor, H AU - Sadat-Ebrahimi, SE AU - Amanzadeh, Y AU - Izaddoost, M AU - Givi, E AD - Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran KW - < KW - i> KW - Trigonella foenum-graecum< KW - /i> KW - Amino acid KW - Diabetes KW - 4-hydroxyisoleucine UR - http://jmp.ir/article-1-505-fa.html ER -