%0 Journal Article %A Teimori, M %A Montasser Kouhsari, S %A Ghafarzadegan, R %A Hajiaghaee, R %T Study of Hypoglycemic Effect of Juglans regia Leaves and its Mechanism %J Journal of Medicinal Plants %V 9 %N 33 %U http://jmp.ir/article-1-514-en.html %R %D 2010 %K <, i>, Juglans regia<, /i>, Blood Glucose, insulin, glut-4, α-Glucosidase, %X Background: Hypoglycemic effect of Juglans regia leaves was reported in other previous researches. Objective: To investigate the mechanism of hypoglycemic action of Julans regia Leaves Methanolic Extract (JRLME), we designed the following study. Methods: Male wistar rats were classified in five groups, each of six, including alloxan-induced diabetic rats treated with JRLME at doses of 250 (D+JRLMEa), 500 mg/kg (D+JRLMEb), Acarbose at dose 20 mg/kg (D+Ac), vehicle alone (DC) and normal rats treated with vehicle alone (NC). The Postprandial blood glucose level was examined in a short time (after a single dose treatment) and a long time (after three weeks daily treatment) model. After three weeks of treatment, all groups’ animals were killed to dissect pancreatic and myocardium for RNA extraction and RT-PCR, to assess insulin and glut-4 genes expression, respectively. In addition, other normal rats were killed to extract intestinal brush border membrane for α-glucosidase solution preparation. The effect of JRLMEa on α-glucosidase activity was evaluated by an in vitro method, compared to Acarbose as a reference drug. Results: The plant extract had a significant hypoglycemic action in both short and long term models. Decrease in PBG level was the most at 8 hour after treatment and more in D+Ac (53%), D+JRLMEa (40%) and D+JRLMEb (29%) versus DC, respectively. There was also permanent PBG reduction in treated groups in comparison with the DC one during long term period. Conclusion: We did not find change in the insulin and glut-4 genes expression. On the other hand, the in vitro assay of α-glucosidase activity displayed inhibitory action of JRLME, like Acarbose, but less effectively. %> http://jmp.ir/article-1-514-en.pdf %P 57-65 %& 57 %! %9 Research %L A-10-218-2 %+ Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal plants, ACECR, Karaj %G eng %@ 2717-204X %[ 2010