%0 Journal Article %A Qavami, N %A Azizi, M %A Yazdian, F %A Qaderi, A %A Nemati, H %T Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method %J Journal of Medicinal Plants %V 17 %N 65 %U http://jmp.ir/article-1-2087-fa.html %R %D 2018 %K Papaver bracteatum, Lindl. Hairy root, Sonication-assisted, Agrobacterium-mediated transformation, Thebaine, %X Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids. Objective: To evaluate the hairy root culture as a novel method for thebaine production. Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT). Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers. Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers. %> http://jmp.ir/article-1-2087-fa.pdf %P 73-82 %& 73 %! %9 Research %L A-10-1164-3 %+ Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran %G eng %@ 2717-204X %[ 2018