Background: Candida albicans is a opportunistic fangi that causes disease in immunosupress patients and can involves different organs. Zingiber officinale Rosc. has strengthening effect of memory, decreases blood sugar and it has hypotension and antimicrobial effects. Objective: Our point in this research is effection of Zingiber officinale Rosc. essential oil against 25 fluconazole resistant vaginal isolates of candida albicans. Methods: There are many different methods for evaluating of drugs sensitiveness in fungi that in this research we used broth microdilution method. in this manner we extracted the essential oil of Zingiber officinale Rosc. by means of Clevenger apparatus and then this essential oil effected on Candida albicans isolates in microplate wells and results were studied. Results: the number of samples that were used in this research was twenty five. Three isolates didn't grow up to the dilution of 1/32nd, four isolates up to the dilution of 1/64th, eleven isolates up to the dilution of 1/128th and seven isolates up to the dilutron of 1/256th. Conclusion: in view of the fact that Zingiber officialie Rosc. essential oil was effective against all isolates of candida albicans, we can present an effective essential oil and we recommend antifungal activity of that in clinical studies.