BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
URL: http://jmp.ir/article-1-534-fa.html
1، B Habibi-Khaniani2 ، K Baghalian3 ، S Shahnazi4 ، H Rassouli4 ، H Naghdi Badi4 
2- Department of Agronomy and Plant Breeding, Islamic Azad University of Dezful
3- Department of Horticulture, Islamic Azad University- Karaj Branch
4- Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj
Background: Garlic is a valuable medicinal plant with variability in desirable morphological and physiological characteristics. The analysis of genetic diversity plays an important role in breeding programs. The RAPD technique could be very effective in detecting genetic variation in garlic.
Objective: The objective of the present work was to detect molecular polymorphism among Iranian garlic ecotypes by RAPD technique.
Methods: Random Amplified Polymorphic DNA (RAPD) marker utilized to assess the genetic diversity among twenty-two different ecotypes of the Iranian garlic. Genomic DNA was extracted from young leaves using Dellaporta method. RAPD amplification was performed using ten 10-mers arbitrary primers.
Results: Results indicated that five out of ten pair primers had no amplification. A total of 35 RAPD bands were produced, 31 of which (88.5%) were polymorphic. The similarity matrices and dendrogram were obtained using UPGMA algorithms.
دریافت: 1387/8/30 | پذیرش: 1387/11/30 | انتشار: 1388/3/31
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
