year 22, Issue 85 (3-2023)                   J. Med. Plants 2023, 22(85): 25-44 | Back to browse issues page

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1- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran
2- Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran ,
Abstract:   (1126 Views)
Background: Scrophularia oblongifolia belongs to the Scrophulariaceae family, commonly known as the water figwort in Iran. It has been known as a traditional medicinal plant in Iran. Objective: This study was designed to investigate the phytochemicals and antimicrobial activity of ethyl acetate, methanol, MeOH: H2O (50:50 v/v), and n-hexane extract of Scrophularia oblongifolia aerial parts, growing in the Kangavar region in the Kermanshah province of Iran. Methods: Compounds of ethyl acetate extract were isolated by chromatographic techniques (HPLC-PDA-MS/MS-Q-TOF) and their structures were elucidated by spectral methods (1D and 2D NMR). The antifungal and antibacterial activities of some new compounds and ethyl acetate, methanol, and n-hexane extract were evaluated. Results: Two new polyphenol iridoid glycosides with phenylpropanoid moiety namely scrophuloside A5 and scrophuloside A6; two known phenylpropanoid glycosides namely scrophuloside A3 and scrophuloside A4, and two known iridoid glycosides namely aucubin and harpagoside were isolated from S. oblongifolia. Pure compounds showed high antifungal activities with MIC values ranging from 0.35 to 0.74 µg/ml against C. albicans and high antibacterial activities with MIC values ranging from 0.70 to 1.48 µg/ml against G. vajinalis. n-Hexan extract showed moderate antibacterial and antifungal activity, while methanol and methanol:H2O (50:50 v/v) extract showed no antimicrobial activities. Conclusion: Based on these findings, compounds isolated from S. oblongifolia have demonstrated potential antibacterial and antifungal activities.
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Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2022/10/21 | Accepted: 2023/02/21 | Published: 2023/03/1

1. Yadav R and Agarwala M. Phytochemical analysis of some medicinal plants. J. Phytology. 2011; 3(12): 10-14.
2. Arunkumar S and Muthuselvam M. Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. World J Agri. Sci. 2009; 5(5): 572-576.
3. Asgharian P, Heshmati Afshar F, Asnaashari S, Lotfipour F, Baradaran B, Zolali E, Nikkhah E and Delazar A. Evaluation of various biological activities of the aerial parts of Scrophularia frigida growing in Iran. Iranian J. Pharm. Res. 2017; 16(1): 277-289.
4. Tohidi B, Rahimmalek M and Arzani A. Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of Thymus species collected from different regions of Iran. Food Chem. 2017; 220: 153-161. [DOI:10.1016/j.foodchem.2016.09.203]
5. Stevenson PC, Simmonds MSJ, Sampson J, Houghton PJ and Grice P. Wound healing activity of acylated iridoid glycosides from Scrophularia nodosa. Phyto. Res. 2002. 16(1): 33-35. [DOI:10.1002/ptr.798]
6. Santos Galíndez J. de, Díaz Lanza A.M.a and Fernandez Matellano L. Biologically active substances from the genus Scrophularia. Pharm. Biol. 2002; 40(1): 45-59. [DOI:10.1076/phbi.]
7. Garcia MD, Ahumada MC and Saenz MT. Cytostatic activity of some phenolic acids of Scrophularia frutescens L. var. frutescens. Z Naturforsch C. 1998; 53(11-12): p. 1093-1095. [DOI:10.1515/znc-1998-11-1225]
8. Brodmann J, Emer D and Ayasse M. Pollinator attraction of the wasp‐flower Scrophularia umbrosa (Scrophulariaceae). Plant Biol. 2012; 14(3): 500-505. [DOI:10.1111/j.1438-8677.2011.00525.x]
9. Pasdaran A and Hamedi A. The genus Scrophularia: a source of iridoids and terpenoids with a diverse biological activity. Pharm. Biol. 2017; 55(1): 2211-2233. [DOI:10.1080/13880209.2017.1397178]
10. Atar F. Notes on The Genus Scrophularia L. (Scrophularlaceae) in Iran. The Iranian J. Bot. 2006. 12(2): 136-143.
11. Catlin BW. Gardnerella vaginalis: characteristics, clinical considerations, and controversies. Clin. Microbiol. Rev. 1992; 5(3): 213-237. [DOI:10.1128/CMR.5.3.213]
12. Han M-F, Zhang X, Zhang L-Q and. Li Y-M. Iridoid and phenylethanol glycosides from Scrophularia umbrosa with inhibitory activity on nitric oxide production. Phytochem. Lett 2018; 28: 37-41. [DOI:10.1016/j.phytol.2018.09.011]
13. Abdollahi M. and Soltaninejad K. A review on toxicity of plants in human. Med. Plants 2002; 1(3): 1-12.
14. Dina NE, Gherman AMR, Chiş V, Sârbu C, Wieser A, Bauer D and Haisch C. Characterization of clinically relevant fungi via SERS fingerprinting assisted by novel chemometric models. Anal. Chem. 2018; 90(4): 2484-2492. [DOI:10.1021/acs.analchem.7b03124]
15. Kerdar T, Moradkhani S and Dastan D. Phytochemical and biological studies of Scrophularia striata from Ilam. Jundishapur J. Nat. Pharm. Prod. 2018; 13(3): 62705-62712. [DOI:10.5812/jjnpp.62705]
16. Albuquerque BR, Prieto MA, Barreiro MF, Rodrigues A, Curran TP, Barros L and Ferreira ICFR. Catechin-based extract optimization obtained from Arbutus unedo L. fruits using maceration/microwave/ultrasound extraction techniques. Ind. Crops Prod. 2017; 95: 404-415. [DOI:10.1016/j.indcrop.2016.10.050]
17. Ghaderi L, Aliahmadi A, Nejad Ebrahimi S and Rafati H. Effective Inhibition and eradication of Pseudomonas aeruginosa biofilms by Satureja khuzistanica essential oil nanoemulsion. J. Drug. Deliv. Sci. Tec. 2021; 61: 102260. [DOI:10.1016/j.jddst.2020.102260]
18. Zeng X, Guo F and Ouyang D. A review of the pharmacology and toxicology of aucubin. Fitoterapia 2020; 140: 104443. [DOI:10.1016/j.fitote.2019.104443]
19. Dıaz AM, Abad MJ, Fernández L,. Silván AM, Santos JDe and Bermejo P. Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro anti-inflammatory activity. Life Sci. 2004; 74(20): 2515-2526. [DOI:10.1016/j.lfs.2003.10.008]
20. Georgiev MI, Ivanovska N, Alipieva K, Dimitrova P and Verpoorte R. Harpagoside: from Kalahari Desert to pharmacy shelf. Phytochem. 2013; 92: 8-15. [DOI:10.1016/j.phytochem.2013.04.009]
21. Berhe AB. Phytochemical Investigation And Antimicrobial Activity of Leaf Extracts of Stereospermum kunthianum. 2018; Haramaya University.
22. Mansouri M, Moallem SA, Asili J and Etemad L. Cytotoxic and apoptotic effects of Scrophularia umbrosa dumort extract on MCF-7 breast cancer and 3T3 cells. Rep. Biochem. Mol. Biol. 2019; 8(1): 79-84.
23. Damtoft S, Jensen SR, Jessen CU and Knudsen TB. Late stages in the biosynthesis of aucubin in Scrophularia. Phytochem. 1993; 33(5): 1089-1093. [DOI:10.1016/0031-9422(93)85028-P]

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