1- Department of Clinical Biochemistry, Faculty of Medicine, Qom University of Medical Sciences
2- Department of Phamacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences , shams@tums.ac.ir
3- Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences
4- Department of Phamacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences
Abstract: (6264 Views)
Background: There are some reports regarding antidiabetic activity of 4-hydroxyisoleucine, however its hypoglycemic property must be proven completely before presentating as a new drug. Preparing suitable amount of 4-hydroxyisoleucine is nesessary for supporting in vivo studies. Objective: The purpose of the present study was isolation of 4-hydroxyisoleucine, comparing 4-hydroxyisoleucine content in two samples of Fenugreek seeds collected form two distinct parts of Iran and finally the effect of germination and supplementation by isoleucine on 4-hydroxyisoleucine yield. Methods: 4-hydroxyisoleucine was successfully isolated passing seed amino acid extract through ion exchange and silica gel column and then crystallization on methanol. 4-hydroxyisoleucine content in various samples was assessed by GC. Results: Seeds from Northwestern of Iran had higher amount of 4-hydroxyisoleucine in comparison to Central Iran. Furthermore yields from germinating seeds were 2 folds higher than the dried seeds, and could be increased 6 fold by inclusion of isoleucine supplement in the irrigation water. Conclusion: simple procedures can be engaged for improving yields of 4-hydroxyisoleucine isolated from fenugreek up to six-fold by using of germinated seeds supplemented with isoleucine.
Type of Study:
Research |
Subject:
Pharmacognosy & Pharmaceutics Received: 2006/05/15 | Accepted: 2008/01/12 | Published: 2010/06/19